This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fortin, J.-F.
Right arrow Articles by Tremblay, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fortin, J.-F.
Right arrow Articles by Tremblay, M. J.

 Previous Article  |  Next Article 

J Virol, March 1998, p. 2105-2112, Vol. 72, No. 3
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

T Cells Expressing Activated LFA-1 Are More Susceptible to Infection with Human Immunodeficiency Virus Type 1 Particles Bearing Host-Encoded ICAM-1

Jean-François Fortin, Réjean Cantin, and Michel J. Tremblay*

Centre de Recherche en Infectiologie, Centre Hospitalier Universitaire de Québec, Pavillon CHUL, and Département de Microbiologie, Faculté de Médecine, Université Laval, Ste-Foy, Québec, Canada G1V 4G2

Received 13 October 1997/Accepted 6 December 1997

The incorporation of host-derived proteins in nascent human immunodeficiency virus type 1 (HIV-1) particles is a well-established phenomenon. We recently demonstrated that the physical presence of host-encoded ICAM-1 glycoproteins on HIV-1 leads to a significant increase in virus infectivity in an ICAM-1/LFA-1-dependent fashion (J.-F. Fortin, R. Cantin, G. Lamontagne, and M. Tremblay, J. Virol. 71:3588-3596, 1997). We show here that conversion of LFA-1 to high affinity for ICAM-1 with the use of anti-LFA-1 antibodies (clones NKI-L16 and MEM83) markedly enhances the susceptibility of different target T-lymphoid cell lines, as well as of primary peripheral blood mononuclear cells, to infection by ICAM-1-bearing HIV-1 particles (6- to 95-fold). It is known that T-cell receptor (TCR) cross-linking induces a transient increase in LFA-1 affinity for ICAM-1. Treatment of peripheral blood mononuclear cells with anti-TCR antibodies (clone OKT3) resulted in a transient increase in susceptibility to infection by ICAM-1-positive virions that parallels the previously reported kinetics of the LFA-1/ICAM-1 adhesion mechanism. Our results led us to postulate that the strong interaction taking place between virally incorporated ICAM-1 and cell surface-activated LFA-1 markedly enhances the efficiency of virus binding and entry, thus favoring greater infection by ICAM-1-bearing HIV-1 particles. In view of the knowledge that primary HIV-1 isolates harbor host-derived ICAM-1 on their surfaces, these results provide new information about the role of host-derived ICAM-1 in the life cycle of HIV-1 and how it could positively modulate the dynamics of the viral infection, mainly in cellular compartments, such as the lymphoid tissues, where the level of cellular activation is high and where the probability of encountering a T cell expressing the activated LFA-1 form is also elevated.

* Corresponding author. Mailing address: Centre de Recherche en Infectiologie, RC709, Centre Hospitalier Universitaire de Québec, Pavillon CHUL, 2705 boul. Laurier, Ste-Foy, Québec, Canada G1V 4G2. Phone: (418) 654-2705. Fax: (418) 654-2715. E-mail: Michel.J.Tremblay{at}crchul.ulaval.ca.




This article has been cited by other articles:

  • Tardif, M. R., Gilbert, C., Thibault, S., Fortin, J.-F., Tremblay, M. J. (2009). LFA-1 Antagonists as Agents Limiting Human Immunodeficiency Virus Type 1 Infection and Transmission and Potentiating the Effect of the Fusion Inhibitor T-20. Antimicrob. Agents Chemother. 53: 4656-4666 [Abstract] [Full Text]  
  • Segura, M. M., Garnier, A., Di Falco, M. R., Whissell, G., Meneses-Acosta, A., Arcand, N., Kamen, A. (2008). Identification of Host Proteins Associated with Retroviral Vector Particles by Proteomic Analysis of Highly Purified Vector Preparations. J. Virol. 82: 1107-1117 [Abstract] [Full Text]  
  • Jolly, C., Mitar, I., Sattentau, Q. J. (2007). Adhesion Molecule Interactions Facilitate Human Immunodeficiency Virus Type 1-Induced Virological Synapse Formation between T Cells. J. Virol. 81: 13916-13921 [Abstract] [Full Text]  
  • Segura, E., Guerin, C., Hogg, N., Amigorena, S., Thery, C. (2007). CD8+ Dendritic Cells Use LFA-1 to Capture MHC-Peptide Complexes from Exosomes In Vivo. J. Immunol. 179: 1489-1496 [Abstract] [Full Text]  
  • Bobardt, M. D., Chatterji, U., Selvarajah, S., Van der Schueren, B., David, G., Kahn, B., Gallay, P. A. (2007). Cell-Free Human Immunodeficiency Virus Type 1 Transcytosis through Primary Genital Epithelial Cells. J. Virol. 81: 395-405 [Abstract] [Full Text]  
  • Kim, S.-j., Nair, A. M., Fernandez, S., Mathes, L., Lairmore, M. D. (2006). Enhancement of LFA-1-Mediated T Cell Adhesion by Human T Lymphotropic Virus Type 1 p12I1. J. Immunol. 176: 5463-5470 [Abstract] [Full Text]  
  • Kolegraff, K., Bostik, P., Ansari, A. A. (2006). Characterization and role of lentivirus-associated host proteins.. Exp. Biol. Med. 231: 252-263 [Abstract] [Full Text]  
  • Tardif, M. R., Tremblay, M. J. (2005). LFA-1 Is a Key Determinant for Preferential Infection of Memory CD4+ T Cells by Human Immunodeficiency Virus Type 1. J. Virol. 79: 13714-13724 [Abstract] [Full Text]  
  • Tardif, M. R., Tremblay, M. J. (2005). Regulation of LFA-1 Activity through Cytoskeleton Remodeling and Signaling Components Modulates the Efficiency of HIV Type-1 Entry in Activated CD4+ T Lymphocytes. J. Immunol. 175: 926-935 [Abstract] [Full Text]  
  • Cantin, R., Methot, S., Tremblay, M. J. (2005). Plunder and Stowaways: Incorporation of Cellular Proteins by Enveloped Viruses. J. Virol. 79: 6577-6587 [Full Text]  
  • Holm, G. H., Gabuzda, D. (2005). Distinct Mechanisms of CD4+ and CD8+ T-Cell Activation and Bystander Apoptosis Induced by Human Immunodeficiency Virus Type 1 Virions. J. Virol. 79: 6299-6311 [Abstract] [Full Text]  
  • Roy, J., Martin, G., Giguere, J.-F., Belanger, D., Petrin, M., Tremblay, M. J. (2005). HIV Type 1 Can Act as an APC upon Acquisition from the Host Cell of Peptide-Loaded HLA-DR and CD86 Molecules. J. Immunol. 174: 4779-4788 [Abstract] [Full Text]  
  • Beausejour, Y., Tremblay, M. J. (2004). Interaction between the Cytoplasmic Domain of ICAM-1 and Pr55Gag Leads to Acquisition of Host ICAM-1 by Human Immunodeficiency Virus Type 1. J. Virol. 78: 11916-11925 [Abstract] [Full Text]  
  • Giguere, J.-F., Tremblay, M. J. (2004). Statin Compounds Reduce Human Immunodeficiency Virus Type 1 Replication by Preventing the Interaction between Virion-Associated Host Intercellular Adhesion Molecule 1 and Its Natural Cell Surface Ligand LFA-1. J. Virol. 78: 12062-12065 [Abstract] [Full Text]  
  • Giguere, J.-F., Bounou, S., Paquette, J.-S., Madrenas, J., Tremblay, M. J. (2004). Insertion of Host-Derived Costimulatory Molecules CD80 (B7.1) and CD86 (B7.2) into Human Immunodeficiency Virus Type 1 Affects the Virus Life Cycle. J. Virol. 78: 6222-6232 [Abstract] [Full Text]  
  • Bobardt, M. D., Salmon, P., Wang, L., Esko, J. D., Gabuzda, D., Fiala, M., Trono, D., Van der Schueren, B., David, G., Gallay, P. A. (2004). Contribution of Proteoglycans to Human Immunodeficiency Virus Type 1 Brain Invasion. J. Virol. 78: 6567-6584 [Abstract] [Full Text]  
  • Tardif, M. R., Tremblay, M. J. (2003). Presence of Host ICAM-1 in Human Immunodeficiency Virus Type 1 Virions Increases Productive Infection of CD4+ T Lymphocytes by Favoring Cytosolic Delivery of Viral Material. J. Virol. 77: 12299-12309 [Abstract] [Full Text]  
  • Callahan, M. K., Popernack, P. M., Tsutsui, S., Truong, L., Schlegel, R. A., Henderson, A. J. (2003). Phosphatidylserine on HIV Envelope Is a Cofactor for Infection of Monocytic Cells. J. Immunol. 170: 4840-4845 [Abstract] [Full Text]  
  • Giguere, J.-F., Paquette, J.-S., Bounou, S., Cantin, R., Tremblay, M. J. (2002). New Insights into the Functionality of a Virion-Anchored Host Cell Membrane Protein: CD28 Versus HIV Type 1. J. Immunol. 169: 2762-2771 [Abstract] [Full Text]  
  • Bounou, S., Leclerc, J. E., Tremblay, M. J. (2002). Presence of Host ICAM-1 in Laboratory and Clinical Strains of Human Immunodeficiency Virus Type 1 Increases Virus Infectivity and CD4+-T-Cell Depletion in Human Lymphoid Tissue, a Major Site of Replication In Vivo. J. Virol. 76: 1004-1014 [Abstract] [Full Text]  
  • Johnson, D. C., Huber, M. T. (2002). Directed Egress of Animal Viruses Promotes Cell-to-Cell Spread. J. Virol. 76: 1-8 [Full Text]  
  • Cantin, R., Martin, G., Tremblay, M. J. (2001). A novel virus capture assay reveals a differential acquisition of host HLA-DR by clinical isolates of human immunodeficiency virus type 1 expanded in primary human cells depending on the nature of producing cells and the donor source. J. Gen. Virol. 82: 2979-2987 [Abstract] [Full Text]  
  • Hioe, C. E., Chien, P. C. Jr., Lu, C., Springer, T. A., Wang, X.-H., Bandres, J., Tuen, M. (2001). LFA-1 Expression on Target Cells Promotes Human Immunodeficiency Virus Type 1 Infection and Transmission. J. Virol. 75: 1077-1082 [Abstract] [Full Text]  
  • Olinger, G. G., Saifuddin, M., Spear, G. T. (2000). CD4-Negative Cells Bind Human Immunodeficiency Virus Type 1 and Efficiently Transfer Virus to T Cells. J. Virol. 74: 8550-8557 [Abstract] [Full Text]  
  • Mouland, A. J., Mercier, J., Luo, M., Bernier, L., DesGroseillers, L., Cohen, E. A. (2000). The Double-Stranded RNA-Binding Protein Staufen Is Incorporated in Human Immunodeficiency Virus Type 1: Evidence for a Role in Genomic RNA Encapsidation. J. Virol. 74: 5441-5451 [Abstract] [Full Text]  
  • Fujiwara, M., Tsunoda, R., Shigeta, S., Yokota, T., Baba, M. (1999). Human Follicular Dendritic Cells Remain Uninfected and Capture Human Immunodeficiency Virus Type 1 through CD54-CD11a Interaction. J. Virol. 73: 3603-3607 [Abstract] [Full Text]  
  • Lee, B., Sharron, M., Blanpain, C., Doranz, B. J., Vakili, J., Setoh, P., Berg, E., Liu, G., Guy, H. R., Durell, S. R., Parmentier, M., Chang, C. N., Price, K., Tsang, M., Doms, R. W. (1999). Epitope Mapping of CCR5 Reveals Multiple Conformational States and Distinct but Overlapping Structures Involved in Chemokine and Coreceptor Function. J. Biol. Chem. 274: 9617-9626 [Abstract] [Full Text]  
  • Paquette, J.-S., Fortin, J.-F., Blanchard, L., Tremblay, M. J. (1998). Level of ICAM-1 Surface Expression on Virus Producer Cells Influences both the Amount of Virion-Bound Host ICAM-1 and Human Immunodeficiency Virus Type 1 Infectivity. J. Virol. 72: 9329-9336 [Abstract] [Full Text]  
  • Barbeau, B., Fortin, J.-F., Genois, N., Tremblay, M. J. (1998). Modulation of Human Immunodeficiency Virus Type 1-Induced Syncytium Formation by the Conformational State of LFA-1 Determined by a New Luciferase-Based Syncytium Quantitative Assay. J. Virol. 72: 7125-7136 [Abstract] [Full Text]  
  • Bounou, S., Dumais, N., Tremblay, M. J. (2001). Attachment of Human Immunodeficiency Virus-1 (HIV-1) Particles Bearing Host-encoded B7-2 Proteins Leads to Nuclear Factor-kappa B- and Nuclear Factor of Activated T Cells-dependent Activation of HIV-1 Long Terminal Repeat Transcription. J. Biol. Chem. 276: 6359-6369 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»