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J Virol, March 1998, p. 2010-2021, Vol. 72, No. 3
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Genome Sequence of Herpes Simplex Virus Type 2

Aidan Dolan,* Fiona E. Jamieson, Charles Cunningham, Barbara C. Barnett,dagger and Duncan J. McGeoch

MRC Virology Unit, Institute of Virology, Glasgow G11 5JR, United Kingdom

Received 7 October 1997/Accepted 21 November 1997

The genomic DNA sequence of herpes simplex virus type 2 (HSV-2) strain HG52 was determined as 154,746 bp with a G+C content of 70.4%. A total of 74 genes encoding distinct proteins was identified; three of these were each present in two copies, within major repeat elements of the genome. The HSV-2 gene set corresponds closely with that of HSV-1, and the HSV-2 sequence prompted several local revisions to the published HSV-1 sequence (D. J. McGeoch, M. A. Dalrymple, A. J. Davison, A. Dolan, M. C. Frame, D. McNab, L. J. Perry, J. E. Scott, and P. Taylor, J. Gen. Virol. 69:1531-1574, 1988). No compelling evidence for the existence of any additional protein-coding genes in HSV-2 was identified.

* Corresponding author. Mailing address: MRC Virology Unit, Institute of Virology, Church St., Glasgow G11 5JR, United Kingdom. Phone: 44 141-330-4633. Fax: 44 141-337-2236. E-mail: a.dolan{at}vir.gla.ac.uk.

dagger Present address: Leukaemia and Cancer Research Fund, Yorkhill NHS Trust, Glasgow G3 8SJ, United Kingdom.




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