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J Virol, March 1998, p. 1910-1917, Vol. 72, No. 3
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Temporal Gene Regulation of the Channel Catfish Virus (Ictalurid Herpesvirus 1)dagger

Suming HuangDagger and Larry A. Hanson*

College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi 39762

Received 9 June 1997/Accepted 24 November 1997

To identify promoter regions that impart differential temporal regulation of channel catfish virus (CCV) genes, the transcriptional kinetics of an immediate-early gene and prospective early and late genes were characterized. A cDNA clone, designated IE3C, representing a third immediate-early transcript was identified. The 5' end of the IE3C transcript was mapped to nucleotides 15,368 and 131,043 in the terminal repeat regions of the CCV genome. The full length of the transcript represented by the IE3C clone is 1,412 bp, and it most likely codes for the protein specified by open reading frame (ORF) 12. The putative product of ORF12 contains a consensus RING finger metal binding motif (C3HC4 structure). Temporal expression studies, in conjunction with protein synthesis and DNA replication inhibition, demonstrated that the IE3C transcript belongs to an immediate-early kinetic class, the ORF5 transcript is a member of the early kinetic class, and ORF39 and ORF46 are true late-kinetic-class genes. Additionally, we demonstrated that ORF38 transcription overlaps ORF39 and the products presumably share the same poly(A) signal. The 5' ends of the transcripts encoding ORF38, ORF39, and ORF46 were mapped to nucleotides 44,862, 45,254, and 59,644, respectively, and potential transcriptional control elements were located.

* Corresponding author. Mailing address: College of Veterinary Medicine, Mississippi State University, P.O. Box 9825, Mississippi State, MS 39762. Phone: (601) 325-1202. Fax: (601) 325-1031. E-mail: hanson{at}cvm.msstate.edu.

dagger Mississippi Agricultural and Forestry Experiment Station publication J-9180.

Dagger Present address: Department of Medicine/Hematology, Vanderbilt University Medical Center, Nashville, TN 37232-2279.




This article has been cited by other articles:

  • Kunec, D., Hanson, L. A., van Haren, S., Nieuwenhuizen, I. F., Burgess, S. C. (2008). An Overlapping Bacterial Artificial Chromosome System That Generates Vectorless Progeny for Channel Catfish Herpesvirus. J. Virol. 82: 3872-3881 [Abstract] [Full Text]  
  • Stingley, R. L., Gray, W. L. (2000). Transcriptional regulation of the channel catfish virus genome direct repeat region. J. Gen. Virol. 81: 2005-2010 [Abstract] [Full Text]  


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