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J Virol, February 1998, p. 959-964, Vol. 72, No. 2
MedImmune, Inc., Gaithersburg, Maryland
20878,1 and
Department of Medicine,
University of Rochester School of Medicine and Dentistry, Rochester,
New York 146422
Received 24 July 1997/Accepted 3 November 1997
Human papillomavirus type 16 (HPV-16) is strongly associated with
the development of cervical cancer. Studies of model systems with
animal papillomaviruses have demonstrated the importance of
neutralizing antibodies in preventing papillomavirus-associated disease. The assessment of neutralizing antibody responses against HPV-16, previously hampered by the lack of a viral source, was enabled
by the recent propagation of an HPV-16 stock in xenografted severe
combined immunodeficiency (SCID) mice. HPV-16 infection of an
immortalized human keratinocyte cell line was demonstrated by detection
of an HPV-16-specific spliced mRNA amplified by reverse transcriptase
PCR. Infection was blocked by preincubation of the virus with antiserum
generated against HPV-16 virus-like particles (VLPs) composed of the
major capsid protein, L1. To examine potential cross-neutralizing
activity among the different genital HPV types, rabbit antisera to L1
VLPs corresponding to HPV-6, -11, -18, -31, -33, -35, -39, and -45 were
assayed for the ability to block the HPV-16 infection of cultured
cells. Antiserum raised against HPV-33 L1 VLPs was the only
heterologous antiserum which inhibited HPV-16 infection. Thus, a
neutralization assay for HPV-16 may help to characterize the components
required to compose a broadly efficacious genital HPV vaccine.
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
In Vitro Infection and Type-Restricted
Antibody-Mediated Neutralization of Authentic Human Papillomavirus
Type 16
*
Corresponding author. Mailing address: 35 W. Watkins
Mill Rd., Gaithersburg, MD 20878. Phone: (301) 417-0770. Fax: (301)
527-4200. E-mail: whitew{at}medimmune.com.
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