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J Virol, February 1998, p. 1704-1708, Vol. 72, No. 2
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Coadministration of DNA Encoding Interleukin-6 and Hemagglutinin Confers Protection from Influenza Virus Challenge in Mice

Diane L. Larsen,¹ Naomi Dybdahl-Sissoko,¹ Martha W. McGregor,¹ Robert Drape,² Veronica Neumann,² William F. Swain,² D. Paul Lunn,³ and Christopher W. Olsen^1,*

Department of Pathobiological Sciences¹ and Department of Medical Sciences, School of Veterinary Medicine, University of WisconsinMadison, Madison, Wisconsin 53706,³ and PowderJect Vaccines, Inc., Madison, Wisconsin 53711²

Received 21 July 1997/Accepted 21 October 1997

This study was conducted to investigate whether Accell gene gun coadministration of DNA encoding human interleukin-6 (IL-6) would enhance protective immune responses in mice to an equine influenza A virus hemagglutinin (HA) DNA vaccine. Mice that received HA DNA alone exhibited accelerated clearance of homologous challenge virus but were not protected from infection. In contrast, mice that received both HA and IL-6 DNA had no detectable virus in their lungs after challenge. These results strongly support the use of IL-6 as a cytokine adjuvant in DNA vaccination.

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^* Corresponding author. Mailing address: Department of Pathobiological Sciences, School of Veterinary Medicine, University of WisconsinMadison, 2015 Linden Dr. West, Madison, WI 53706. Phone: (608) 265-8681. Fax: (608) 263-0438. E-mail: olsenc{at}svm.vetmed.wisc.edu.

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