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J Virol, February 1998, p. 1688-1690, Vol. 72, No. 2
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Protective Immune Response to Foot-and-Mouth Disease Virus with VP1 Expressed in Transgenic Plants

C. Carrillo,¹ A. Wigdorovitz,¹ J. C. Oliveros,² P. I. Zamorano,¹ A. M. Sadir,¹ N. Gómez,³ J. Salinas,² J. M. Escribano,³ and M. V. Borca^1,*

Instituto de Virología, C.I.C.V., INTA-Castelar, Buenos Aires, Argentina,¹ and Departamento de Investigaciones Forestales, CIT-INIA, 28040 Madrid,² and Centro de Investigación en Sanidad Animal, CISA-INIA, Valdeolmos, 28130 Madrid,³ Spain

Received 30 July 1997/Accepted 30 October 1997

It has been reported recently that genes encoding antigens of bacterial and viral pathogens can be expressed in plants in a form in which they retain native immunogenic properties. The structural protein VP1 of foot-and-mouth disease virus (FMDV), which has frequently been shown to contain critical epitopes, has been expressed in different vectors and shown to induce virus-neutralizing antibodies and protection in experimental and natural hosts. Here we report the production of transformed plants (Arabidopsis thaliana) expressing VP1. Mice immunized with leaf plant extracts elicited specific antibody responses to synthetic peptides representing amino acid residues 135 to 160 of VP1, to VP1 itself, and to intact FMDV particles. Additionally, all of the immunized mice were protected against challenge with virulent FMDV. To our knowledge, this is the first study showing protection against a viral disease by immunization with an antigen expressed in a transgenic plant.

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^* Corresponding author. Mailing address: Instituto de Virologia, C.I.C.V., INTA-Castelar, CC77 Moron, 1708 Pcia. Buenos Aires, Argentina. Phone: 54 1 621 1676. Fax: 54 1 621 1743.

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