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J Virol, February 1998, p. 1662-1665, Vol. 72, No. 2
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Determinant for Central Nervous System Persistence Localized in the Capsid of Theiler's Murine Encephalomyelitis Virus by Using Recombinant Viruses

Cecilia Adami,1,2,dagger Arthur E. Pritchard,3 Todd Knauf,1,2 Ming Luo,4 and Howard L. Lipton1,2,*

Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University,1 and Division of Neurology, Evanston Hospital,2 Evanston, Illinois; Center for Macromolecular Crystallography, University of Alabama at Birmingham, Birmingham, Alabama 35294-00054; and Department of Biochemistry, University of Colorado Health Sciences Center, Denver, Colorado 802623

Received 18 June 1997/Accepted 20 October 1997

The demyelinating process in Theiler's murine encephalomyelitis virus (TMEV) infection in mice requires virus persistence in the central nervous system. Using recombinant TMEV assembled between the virulent GDVII and less virulent BeAn virus cDNAs, we now provide additional evidence supporting the localization of a persistence determinant to the leader P1 (capsid) sequences. Further, recombinant viruses in which BeAn sequences progressively replaced those of GDVII within the capsid starting at the leader NH2 terminus suggest that a conformational determinant requiring homologous sequences in both the VP2 puff and VP1 loop regions, which are in close contact on the virion surface, might underlie persistence.

* Corresponding author. Mailing address: Division of Neurology, Evanston Hospital, 2650 Ridge Ave., Evanston, IL 60201. Phone: (847) 570-2168. Fax: (847) 570-1568. E-mail: hllipton{at}merle.acns.nwu.edu.

dagger Present address: Department of Experimental Medicine and Biochemical Sciences, Section of Microbiology, University of Perugia Medical School, Perugia, Italy.




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