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J Virol, February 1998, p. 1623-1626, Vol. 72, No. 2
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Essential Roles of NF-kappa B and C/EBP in the Regulation of Intercellular Adhesion Molecule-1 after Respiratory Syncytial Virus Infection of Human Respiratory Epithelial Cell Cultures

B. A. Chini,1 M. A. Fiedler,2 L. Milligan,2 T. Hopkins,2 and J. M. Stark2,*

Division of Pulmonary Medicine, Children's Hospital, Pittsburgh, Pennsylvania,1 and Division of Pulmonary Medicine, Children's Hospital Medical Center, Cincinnati, Ohio2

Received 22 August 1997/Accepted 16 October 1997

To determine the molecular mechanism(s) of respiratory syncytial virus (RSV)-induced intercellular adhesion molecule-1 (ICAM-1) upregulation in respiratory epithelial cells (REC; A549 cell cultures), we investigated the roles of the transcription factors NF-kappa B and C/EBP. Increases in ICAM-1 message required de novo mRNA synthesis. ICAM-1 promoter constructs (luciferase reporter gene) transfected into A549 monolayers demonstrated promoter activation following RSV infection. Activation was abolished by site-specific mutation of the NF-kappa B (-228) or C/EBP (-239) sites. These data support the critical role of the activation of NF-kappa B and C/EBP in RSV-induced ICAM-1 expression by REC.

* Corresponding author. Mailing address: Division of Pulmonary Medicine, Children's Hospital Medical Center, 3333 Burnet Ave. OSB5, Cincinnati, OH 45229-3039. Phone: (513) 636-6771. Fax: (513) 636-4615. E-mail: starj0{at}chmcc.org.




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