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J Virol, February 1998, p. 1504-1515, Vol. 72, No. 2
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
mRNA Instability Elements in the Human
Papillomavirus Type 16 L2 Coding Region
Marcus
Sokolowski,
Wei
Tan,
Marianne
Jellne, and
Stefan
Schwartz*
Microbiology and Tumorbiology Center,
Karolinska Institute, 171 77 Stockholm, Sweden
Received 14 August 1997/Accepted 3 November 1997
Human papillomavirus capsid proteins L1 and L2 are detected only in
terminally differentiated cells, indicating that expression of the L1
and L2 genes is blocked in dividing cells. The results presented here
establish that the human papillomavirus type 16 L2 coding region
contains cis-acting inhibitory sequences. When placed
downstream of a reporter gene, the human papillomavirus type 16 L2
sequence reduced both mRNA and protein levels in an orientation-dependent manner. Deletion analysis revealed that the L2
sequence contains two cis-acting inhibitory RNA regions. We
identified an inhibitory region in the 5'-most 845 nucleotides of L2
that acted by reducing cytoplasmic mRNA stability and a second, weaker
inhibitory region in the 3' end of L2. In contrast, human
papillomavirus type 1 L1 and L2 genes did not encode strong inhibitory
sequences. This result is consistent with observations of high virus
production in human papillomavirus type 1-infected tissue, whereas only
low levels of human papillomavirus type 16 virions are detectable in
infected epithelium. The presence of inhibitory sequences in the L1 and
L2 mRNAs may aid the virus in avoiding the host immunosurveillance and
in establishing persistent infections.
*
Corresponding author. Present address: Department of
Medical Immunology and Microbiology, Biomedical Center, Uppsala
University, P.O. Box 582, 751 23 Uppsala, Sweden. Phone: 4618 471 4928. Fax: 4618 509 876. E-mail: Stefan.Schwartz{at}imim.uu.se.

Present address: Department of Medical Immunology and Microbiology,
BMC, Uppsala University, 751 23 Uppsala, Sweden.

Present address: The John P. Robarts Research Institute, London,
Ontario N6A 5K8, Canada.
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