J Virol, February 1998, p. 1252-1261, Vol. 72, No. 2
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
The Wistar Institute1
and
Division of Neurosurgery,
Received 8 August 1997/Accepted 15 October 1997
The detailed mechanism which governs the choice between herpes
simplex virus (HSV) latency and reactivation remains to be elucidated.
It is probable that altered expression of cellular factors in sensory
neurons leads to induction of HSV gene expression resulting in
reactivation. As an approach to identify novel cellular genes which are
activated or repressed by stimuli that reactivate HSV from latency and
hence may play a role in viral reactivation, RNA from explanted
trigeminal ganglia (TG) was analyzed by differential display reverse
transcription-PCR (DDRT-PCR). Nearly 50 cDNAs whose mRNA level was
modified by the stress of explantation were isolated and sequenced. We
present a listing of a spectrum of altered RNAs, including both known
and unknown sequences. Five of those differentially displayed
transcripts were identified as interferon-related murine TIS7 mRNA.
These results were confirmed in both infected and uninfected ganglia by
quantitative RNase protection assay and immunostaining. Alpha and beta
interferons and interferon regulatory factor-1 (IRF-1) were also
induced by explantation. In addition, we have identified sequences that
correspond to IRF-1 consensus binding sites in both HSV type 1 origins
of replication. Our findings suggest that physiological pathways that
include these cellular factors may be involved in modulating HSV
reactivation.
*
Corresponding author. Mailing address for Jeffry J. Leary: Department of Molecular Virology & Host Defense, SmithKline
Beecham Pharmaceuticals, 1250 S. Collegeville Rd., P.O. Box 5089, Collegeville, PA 19426-0989. Phone: (610) 917-6558. Fax: (610)
917-4170. E-mail: leary{at}sbphrd.com. Mailing address for Shelley L. Berger: The Wistar Institute, 3601 Spruce St., Philadelphia, PA
19104-4268. Phone: (215) 898-3922. Fax: (215) 898-0663. E-mail:
berger{at}wista.wistar.upenn.edu.
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