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J Virol, February 1998, p. 1138-1145, Vol. 72, No. 2
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Regulation of the Human Proliferating Cell Nuclear Antigen Promoter by the Adenovirus E1A-Associated Protein p107

Benjamin H. Lee,1,2 Mingsong Liu,1,dagger and Michael B. Mathews1,3,*

Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724-22081; Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook, Stony Brook, New York 117902; and Department of Biochemistry and Molecular Biology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 071033

Received 23 June 1997/Accepted 5 November 1997

The adenovirus E1A 243R oncoprotein is capable of transactivating the expression of the human proliferating cell nuclear antigen (PCNA) promoter. Mutational analysis of the E1A 243R protein suggested that both its p300/CBP- and p107-binding regions are required for optimal induction of the PCNA promoter (C. Kannabiran, G. F. Morris, C. Labrie, and M. B. Mathews, J. Virol. 67:425-437, 1993). We show that overexpression of p107 antagonizes the induction of PCNA by E1A 243R in transient expression assays. This inhibition is largely independent of p107's ability to interact with E1A 243R, because p107 mutants unable to bind to E1A 243R retain the ability to repress the E1A-activated PCNA promoter. Electrophoretic mobility shift assays with the PCNA promoter detected the presence of p107 in one of the major DNA-protein complexes, EH1, formed with HeLa cell nuclear extracts. Promoter mutations that disrupt the formation of complex EH1 abrogated p107's ability to reverse E1A 243R-induced PCNA expression. The same mutations characterize a sequence important for the binding of transcription factor RFX1 (C. Labrie, G. F. Morris, and M. B. Mathews, Nucleic Acids Res. 23:3732-3741, 1995), implying that p107 antagonizes E1A 243R-induced PCNA expression through this RFX1-binding site. Our data are suggestive of a novel cooperative mechanism for transactivation of PCNA expression, in which E1A 243R relieves transcriptional repression exerted by p107 on the promoter.

* Corresponding author. Present address: Department of Biochemistry and Molecular Biology, New Jersey Medical School, UMDNJ---Newark, 185 S. Orange Ave., Newark, NJ 07103-2714. Phone: (973) 972-4411. Fax: (973) 972-5594. E-mail: mathews{at}umdnj.edu.

dagger Present address: Cardiovascular Research Institute, University of California---San Francisco, San Francisco, CA 94143-0130.




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