This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Herrmann, C. H.
Right arrow Articles by Rice, A. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Herrmann, C. H.
Right arrow Articles by Rice, A. P.

 Previous Article  |  Next Article 

Journal of Virology, December 1998, p. 9881-9888, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Tat-Associated Kinase, TAK, Activity Is Regulated by Distinct Mechanisms in Peripheral Blood Lymphocytes and Promonocytic Cell Lines

Christine H. Herrmann,1,* Richard G. Carroll,2 Ping Wei,3,dagger Katherine A. Jones,3 and Andrew P. Rice1

Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 770301; H. M. Jackson Foundation, Military HIV Research Program, Bethesda, Maryland 208892; and Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 920373

Received 4 August 1998/Accepted 10 September 1998

TAK, a multisubunit cellular protein kinase that specifically associates with the human immunodeficiency virus Tat proteins and hyperphosphorylates the carboxyl-terminal domain of RNA polymerase II, is a cofactor for Tat and mediates its transactivation function. The catalytic subunit of TAK has been identified as cyclin-dependent kinase Cdk9, and its regulatory partner has been identified as cyclin T1; these proteins are also components of positive transcription elongation factor P-TEFb. TAK activity is up-regulated upon activation of peripheral blood lymphocytes and following macrophage differentiation of promonocytic cell lines. We have found that activation of peripheral blood lymphocytes results in increased mRNA and protein levels of both Cdk9 and cyclin T1. Cdk9 and cyclin T1 induction occurred in purified CD4+ primary T cells activated by a variety of stimuli. In contrast, phorbol ester-induced differentiation of promonocytic cell lines into macrophage-like cells produced a large induction of cyclin T1 protein expression from nearly undetectable levels, while Cdk9 protein levels remained at a constant high level. Measurements of cyclin T1 mRNA levels in a promonocytic cell line suggested that regulation of cyclin T1 occurs at a posttranscriptional level. These results suggest that cyclin T1 and TAK function may be required in differentiated monocytes and further show that TAK activity can be regulated by distinct mechanisms in different cell types.

* Corresponding author. Mailing address: Baylor College of Medicine, Division of Molecular Virology, One Baylor Plaza, Houston, TX 77030. Phone: (713) 798-6428. Fax: (713) 798-3490. E-mail: herrmann{at}bcm.tmc.edu.

dagger Present address: 15711 Mahogany Circle, Gaithersburg, MD 20878.

Journal of Virology, December 1998, p. 9881-9888, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ramakrishnan, R., Dow, E. C., Rice, A. P. (2009). Characterization of Cdk9 T-loop phosphorylation in resting and activated CD4+ T lymphocytes. J. Leukoc. Biol. 86: 1345-1350 [Abstract] [Full Text]  
  • Pearson, R., Kim, Y. K., Hokello, J., Lassen, K., Friedman, J., Tyagi, M., Karn, J. (2008). Epigenetic Silencing of Human Immunodeficiency Virus (HIV) Transcription by Formation of Restrictive Chromatin Structures at the Viral Long Terminal Repeat Drives the Progressive Entry of HIV into Latency. J. Virol. 82: 12291-12303 [Abstract] [Full Text]  
  • Jadlowsky, J. K., Nojima, M., Okamoto, T., Fujinaga, K. (2008). Dominant negative mutant cyclin T1 proteins that inhibit HIV transcription by forming a kinase inactive complex with Tat. J. Gen. Virol. 89: 2783-2787 [Abstract] [Full Text]  
  • Hou, T., Ray, S., Brasier, A. R. (2007). The Functional Role of an Interleukin 6-inducible CDK9{middle dot}STAT3 Complex in Human {gamma}-Fibrinogen Gene Expression. J. Biol. Chem. 282: 37091-37102 [Abstract] [Full Text]  
  • Fu, J., Yoon, H.-G., Qin, J., Wong, J. (2007). Regulation of P-TEFb Elongation Complex Activity by CDK9 Acetylation. Mol. Cell. Biol. 27: 4641-4651 [Abstract] [Full Text]  
  • Richter, S. N., Belanger, F., Zheng, P., Rana, T. M. (2006). Dynamics of nascent mRNA folding and RNA-protein interactions: an alternative TAR RNA structure is involved in the control of HIV-1 mRNA transcription. Nucleic Acids Res 34: 4278-4292 [Abstract] [Full Text]  
  • Iankova, I., Petersen, R. K., Annicotte, J.-S., Chavey, C., Hansen, J. B., Kratchmarova, I., Sarruf, D., Benkirane, M., Kristiansen, K., Fajas, L. (2006). Peroxisome Proliferator-Activated Receptor {gamma} Recruits the Positive Transcription Elongation Factor b Complex to Activate Transcription and Promote Adipogenesis. Mol. Endocrinol. 20: 1494-1505 [Abstract] [Full Text]  
  • Liou, L.-Y., Haaland, R. E., Herrmann, C. H., Rice, A. P. (2006). Cyclin T1 but not cyclin T2a is induced by a post-transcriptional mechanism in PAMP-activated monocyte-derived macrophages. J. Leukoc. Biol. 79: 388-396 [Abstract] [Full Text]  
  • Marshall, R. M., Salerno, D., Garriga, J., Grana, X. (2005). Cyclin T1 Expression Is Regulated by Multiple Signaling Pathways and Mechanisms during Activation of Human Peripheral Blood Lymphocytes. J. Immunol. 175: 6402-6411 [Abstract] [Full Text]  
  • Shan, B., Zhuo, Y., Chin, D., Morris, C. A., Morris, G. F., Lasky, J. A. (2005). Cyclin-dependent Kinase 9 Is Required for Tumor Necrosis Factor-{alpha}-stimulated Matrix Metalloproteinase-9 Expression in Human Lung Adenocarcinoma Cells. J. Biol. Chem. 280: 1103-1111 [Abstract] [Full Text]  
  • Lassen, K. G., Bailey, J. R., Siliciano, R. F. (2004). Analysis of Human Immunodeficiency Virus Type 1 Transcriptional Elongation in Resting CD4+ T Cells In Vivo. J. Virol. 78: 9105-9114 [Abstract] [Full Text]  
  • Siliciano, J. D., Siliciano, R. F. (2004). A long-term latent reservoir for HIV-1: discovery and clinical implications. J Antimicrob Chemother 54: 6-9 [Abstract] [Full Text]  
  • Han, Y., Lassen, K., Monie, D., Sedaghat, A. R., Shimoji, S., Liu, X., Pierson, T. C., Margolick, J. B., Siliciano, R. F., Siliciano, J. D. (2004). Resting CD4+ T Cells from Human Immunodeficiency Virus Type 1 (HIV-1)-Infected Individuals Carry Integrated HIV-1 Genomes within Actively Transcribed Host Genes. J. Virol. 78: 6122-6133 [Abstract] [Full Text]  
  • Rohr, O., Marban, C., Aunis, D., Schaeffer, E. (2003). Regulation of HIV-1 gene transcription: from lymphocytes to microglial cells. J. Leukoc. Biol. 74: 736-749 [Abstract] [Full Text]  
  • Lin, X., Irwin, D., Kanazawa, S., Huang, L., Romeo, J., Yen, T. S. B., Peterlin, B. M. (2003). Transcriptional Profiles of Latent Human Immunodeficiency Virus in Infected Individuals: Effects of Tat on the Host and Reservoir. J. Virol. 77: 8227-8236 [Abstract] [Full Text]  
  • Hermankova, M., Siliciano, J. D., Zhou, Y., Monie, D., Chadwick, K., Margolick, J. B., Quinn, T. C., Siliciano, R. F. (2003). Analysis of Human Immunodeficiency Virus Type 1 Gene Expression in Latently Infected Resting CD4+ T Lymphocytes In Vivo. J. Virol. 77: 7383-7392 [Abstract] [Full Text]  
  • Liou, L.-Y., Herrmann, C. H., Rice, A. P. (2002). Transient Induction of Cyclin T1 during Human Macrophage Differentiation Regulates Human Immunodeficiency Virus Type 1 Tat Transactivation Function. J. Virol. 76: 10579-10587 [Abstract] [Full Text]  
  • Fujinaga, K., Irwin, D., Geyer, M., Peterlin, B. M. (2002). Optimized Chimeras between Kinase-Inactive Mutant Cdk9 and Truncated Cyclin T1 Proteins Efficiently Inhibit Tat Transactivation and Human Immunodeficiency Virus Gene Expression. J. Virol. 76: 10873-10881 [Abstract] [Full Text]  
  • Ambrosino, C., Palmieri, C., Puca, A., Trimboli, F., Schiavone, M., Olimpico, F., Ruocco, M. R., di Leva, F., Toriello, M., Quinto, I., Venuta, S., Scala, G. (2002). Physical and Functional Interaction of HIV-1 Tat with E2F-4, a Transcriptional Regulator of Mammalian Cell Cycle. J. Biol. Chem. 277: 31448-31458 [Abstract] [Full Text]  
  • Cook, J. A., August, A., Henderson, A. J. (2002). Recruitment of Phosphatidylinositol 3-Kinase to CD28 Inhibits HIV Transcription by a Tat-Dependent Mechanism. J. Immunol. 169: 254-260 [Abstract] [Full Text]  
  • Martin-Serrano, J., Li, K., Bieniasz, P. D. (2002). Cyclin T1 Expression Is Mediated by a Complex and Constitutively Active Promoter and Does Not Limit Human Immunodeficiency Virus Type 1 Tat Function in Unstimulated Primary Lymphocytes. J. Virol. 76: 208-219 [Abstract] [Full Text]  
  • Kiernan, R. E., Emiliani, S., Nakayama, K., Castro, A., Labbe, J. C., Lorca, T., Nakayama, K.-i., Benkirane, M. (2001). Interaction between Cyclin T1 and SCFSKP2 Targets CDK9 for Ubiquitination and Degradation by the Proteasome. Mol. Cell. Biol. 21: 7956-7970 [Abstract] [Full Text]  
  • Ghose, R., Liou, L.-Y., Herrmann, C. H., Rice, A. P. (2001). Induction of TAK (Cyclin T1/P-TEFb) in Purified Resting CD4+ T Lymphocytes by Combination of Cytokines. J. Virol. 75: 11336-11343 [Abstract] [Full Text]  
  • Marcello, A., Cinelli, R. A. G., Ferrari, A., Signorelli, A., Tyagi, M., Pellegrini, V., Beltram, F., Giacca, M. (2001). Visualization of in Vivo Direct Interaction between HIV-1 TAT and Human Cyclin T1 in Specific Subcellular Compartments by Fluorescence Resonance Energy Transfer. J. Biol. Chem. 276: 39220-39225 [Abstract] [Full Text]  
  • West, M. J., Lowe, A. D., Karn, J. (2001). Activation of Human Immunodeficiency Virus Transcription in T Cells Revisited: NF-{kappa}B p65 Stimulates Transcriptional Elongation. J. Virol. 75: 8524-8537 [Abstract] [Full Text]  
  • Wang, D., de la Fuente, C., Deng, L., Wang, L., Zilberman, I., Eadie, C., Healey, M., Stein, D., Denny, T., Harrison, L. E., Meijer, L., Kashanchi, F. (2001). Inhibition of Human Immunodeficiency Virus Type 1 Transcription by Chemical Cyclin-Dependent Kinase Inhibitors. J. Virol. 75: 7266-7279 [Abstract] [Full Text]  
  • De Luca, A., Russo, P., Severino, A., Baldi, A., Battista, T., Cavallotti, I., De Luca, L., Baldi, F., Giordano, A., Paggi, M. G. (2001). Pattern of Expression of Cyclin T1 in Human Tissues. J. Histochem. Cytochem. 49: 685-692 [Abstract] [Full Text]  
  • Foskett, S. M., Ghose, R., Tang, D. N., Lewis, D. E., Rice, A. P. (2001). Antiapoptotic Function of Cdk9 (TAK/P-TEFb) in U937 Promonocytic Cells. J. Virol. 75: 1220-1228 [Abstract] [Full Text]  
  • Herrmann, C., Mancini, M. (2001). The Cdk9 and cyclin T subunits of TAK/P-TEFb localize to splicing factor-rich nuclear speckle regions. J. Cell Sci. 114: 1491-1503 [Abstract]  
  • Fong, Y. W., Zhou, Q. (2000). Relief of Two Built-In Autoinhibitory Mechanisms in P-TEFb Is Required for Assembly of a Multicomponent Transcription Elongation Complex at the Human Immunodeficiency Virus Type 1 Promoter. Mol. Cell. Biol. 20: 5897-5907 [Abstract] [Full Text]  
  • Barboric, M., Taube, R., Nekrep, N., Fujinaga, K., Peterlin, B. M. (2000). Binding of Tat to TAR and Recruitment of Positive Transcription Elongation Factor b Occur Independently in Bovine Immunodeficiency Virus. J. Virol. 74: 6039-6044 [Abstract] [Full Text]  
  • Bogerd, H. P., Wiegand, H. L., Bieniasz, P. D., Cullen, B. R. (2000). Functional Differences between Human and Bovine Immunodeficiency Virus Tat Transcription Factors. J. Virol. 74: 4666-4671 [Abstract] [Full Text]  
  • Price, D. H. (2000). P-TEFb, a Cyclin-Dependent Kinase Controlling Elongation by RNA Polymerase II. Mol. Cell. Biol. 20: 2629-2634 [Full Text]  
  • Kashanchi, F., Agbottah, E. T., Pise-Masison, C. A., Mahieux, R., Duvall, J., Kumar, A., Brady, J. N. (2000). Cell Cycle-Regulated Transcription by the Human Immunodeficiency Virus Type 1 Tat Transactivator. J. Virol. 74: 652-660 [Abstract] [Full Text]  
  • Fu, T.-J., Peng, J., Lee, G., Price, D. H., Flores, O. (1999). Cyclin K Functions as a CDK9 Regulatory Subunit and Participates in RNA Polymerase II Transcription. J. Biol. Chem. 274: 34527-34530 [Abstract] [Full Text]  
  • GARBER, M.E., WEI, P., JONES, K.A. (1998). HIV-1 Tat Interacts with Cyclin T1 to Direct the P-TEFb CTD Kinase Complex to TAR RNA. Cold Spring Harb Symp Quant Biol 63: 371-380 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»