This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Takimoto, T.
Right arrow Articles by Portner, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takimoto, T.
Right arrow Articles by Portner, A.

 Previous Article  |  Next Article 

Journal of Virology, December 1998, p. 9747-9754, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cytoplasmic Domain of Sendai Virus HN Protein Contains a Specific Sequence Required for Its Incorporation into Virions

Toru Takimoto,1 Tatiana Bousse,1 Elizabeth C. Coronel,1 Ruth Ann Scroggs,1 and Allen Portner1,2,*

Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105,1 and Department of Pathology, The Health Science Center, University of Tennessee, Memphis, Tennessee 381632

Received 29 May 1998/Accepted 31 August 1998

In the assembly of paramyxoviruses, interactions between viral proteins are presumed to be specific. The focus of this study is to elucidate the protein-protein interactions during the final stage of viral assembly that result in the incorporation of the viral envelope proteins into virions. To this end, we examined the specificity of HN incorporation into progeny virions by transiently transfecting HN cDNA genes into Sendai virus (SV)-infected cells. SV HN expressed from cDNA was efficiently incorporated into progeny Sendai virions, whereas Newcastle disease virus (NDV) HN was not. This observation supports the theory of a selective mechanism for HN incorporation. To identify the region on HN responsible for the selective incorporation, we constructed chimeric SV and NDV HN cDNAs and evaluated the incorporation of expressed proteins into progeny virions. Chimera HN that contained the SV cytoplasmic domain fused to the transmembrane and external domains of the NDV HN was incorporated to SV particles, indicating that amino acids in the cytoplasmic domain are responsible for the observed specificity. Additional experiments using the chimeric HNs showed that 14 N-terminal amino acids are sufficient for the specificity. Further analysis identified five consecutive amino acids (residues 10 to 14) that were required for the specific incorporation of HN into SV. These residues are conserved among all strains of SV as well as those of its counterpart, human parainfluenza virus type 1. These results suggest that this region near the N terminus of HN interacts with another viral protein(s) to lead to the specific incorporation of HN into progeny virions.

* Corresponding author. Mailing address: Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, 332 N. Lauderdale, Memphis, TN 38105. Phone: (901) 495-3400. Fax: (901) 523-2622. E-mail: allen.portner{at}stjude.org.

Journal of Virology, December 1998, p. 9747-9754, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kim, S.-H., Yan, Y., Samal, S. K. (2009). Role of the Cytoplasmic Tail Amino Acid Sequences of Newcastle Disease Virus Hemagglutinin-Neuraminidase Protein in Virion Incorporation, Cell Fusion, and Pathogenicity. J. Virol. 83: 10250-10255 [Abstract] [Full Text]  
  • Watanabe, M., Mishin, V. P., Brown, S. A., Russell, C. J., Boyd, K., Babu, Y. S., Taylor, G., Xiong, X., Yan, X., Portner, A., Alymova, I. V. (2009). Effect of Hemagglutinin-Neuraminidase Inhibitors BCX 2798 and BCX 2855 on Growth and Pathogenicity of Sendai/Human Parainfluenza Type 3 Chimera Virus in Mice. Antimicrob. Agents Chemother. 53: 3942-3951 [Abstract] [Full Text]  
  • Runkler, N., Dietzel, E., Moll, M., Klenk, H.-D., Maisner, A. (2008). Glycoprotein targeting signals influence the distribution of measles virus envelope proteins and virus spread in lymphocytes. J. Gen. Virol. 89: 687-696 [Abstract] [Full Text]  
  • Shi, X., Kohl, A., Li, P., Elliott, R. M. (2007). Role of the Cytoplasmic Tail Domains of Bunyamwera Orthobunyavirus Glycoproteins Gn and Gc in Virus Assembly and Morphogenesis. J. Virol. 81: 10151-10160 [Abstract] [Full Text]  
  • Tahara, M., Takeda, M., Yanagi, Y. (2007). Altered Interaction of the Matrix Protein with the Cytoplasmic Tail of Hemagglutinin Modulates Measles Virus Growth by Affecting Virus Assembly and Cell-Cell Fusion. J. Virol. 81: 6827-6836 [Abstract] [Full Text]  
  • Mittler, E., Kolesnikova, L., Strecker, T., Garten, W., Becker, S. (2007). Role of the Transmembrane Domain of Marburg Virus Surface Protein GP in Assembly of the Viral Envelope. J. Virol. 81: 3942-3948 [Abstract] [Full Text]  
  • Oomens, A. G. P., Bevis, K. P., Wertz, G. W. (2006). The Cytoplasmic Tail of the Human Respiratory Syncytial Virus F Protein Plays Critical Roles in Cellular Localization of the F Protein and Infectious Progeny Production. J. Virol. 80: 10465-10477 [Abstract] [Full Text]  
  • Zimmer, G., Bossow, S., Kolesnikova, L., Hinz, M., Neubert, W. J., Herrler, G. (2005). A Chimeric Respiratory Syncytial Virus Fusion Protein Functionally Replaces the F and HN Glycoproteins in Recombinant Sendai Virus. J. Virol. 79: 10467-10477 [Abstract] [Full Text]  
  • Sakaguchi, T., Kato, A., Sugahara, F., Shimazu, Y., Inoue, M., Kiyotani, K., Nagai, Y., Yoshida, T. (2005). AIP1/Alix Is a Binding Partner of Sendai Virus C Protein and Facilitates Virus Budding. J. Virol. 79: 8933-8941 [Abstract] [Full Text]  
  • Ghildyal, R., Li, D., Peroulis, I., Shields, B., Bardin, P. G., Teng, M. N., Collins, P. L., Meanger, J., Mills, J. (2005). Interaction between the respiratory syncytial virus G glycoprotein cytoplasmic domain and the matrix protein. J. Gen. Virol. 86: 1879-1884 [Abstract] [Full Text]  
  • Takimoto, T., Hurwitz, J. L., Coleclough, C., Prouser, C., Krishnamurthy, S., Zhan, X., Boyd, K., Scroggs, R. A., Brown, B., Nagai, Y., Portner, A., Slobod, K. S. (2004). Recombinant Sendai Virus Expressing the G Glycoprotein of Respiratory Syncytial Virus (RSV) Elicits Immune Protection against RSV. J. Virol. 78: 6043-6047 [Abstract] [Full Text]  
  • Oomens, A. G. P., Wertz, G. W. (2004). The Baculovirus GP64 Protein Mediates Highly Stable Infectivity of a Human Respiratory Syncytial Virus Lacking Its Homologous Transmembrane Glycoproteins. J. Virol. 78: 124-135 [Abstract] [Full Text]  
  • Oomens, A. G. P., Megaw, A. G., Wertz, G. W. (2003). Infectivity of a Human Respiratory Syncytial Virus Lacking the SH, G, and F Proteins Is Efficiently Mediated by the Vesicular Stomatitis Virus G Protein. J. Virol. 77: 3785-3798 [Abstract] [Full Text]  
  • Takimoto, T., Taylor, G. L., Connaris, H. C., Crennell, S. J., Portner, A. (2002). Role of the Hemagglutinin-Neuraminidase Protein in the Mechanism of Paramyxovirus-Cell Membrane Fusion. J. Virol. 76: 13028-13033 [Abstract] [Full Text]  
  • Waning, D. L., Schmitt, A. P., Leser, G. P., Lamb, R. A. (2002). Roles for the Cytoplasmic Tails of the Fusion and Hemagglutinin-Neuraminidase Proteins in Budding of the Paramyxovirus Simian Virus 5. J. Virol. 76: 9284-9297 [Abstract] [Full Text]  
  • Takimoto, T., Murti, K. G., Bousse, T., Scroggs, R. A., Portner, A. (2001). Role of Matrix and Fusion Proteins in Budding of Sendai Virus. J. Virol. 75: 11384-11391 [Abstract] [Full Text]  
  • Suzuki, T., Portner, A., Scroggs, R. A., Uchikawa, M., Koyama, N., Matsuo, K., Suzuki, Y., Takimoto, T. (2001). Receptor Specificities of Human Respiroviruses. J. Virol. 75: 4604-4613 [Abstract] [Full Text]  
  • Coronel, E. C., Takimoto, T., Murti, K. G., Varich, N., Portner, A. (2001). Nucleocapsid Incorporation into Parainfluenza Virus Is Regulated by Specific Interaction with Matrix Protein. J. Virol. 75: 1117-1123 [Abstract] [Full Text]  
  • Tao, T., Skiadopoulos, M. H., Davoodi, F., Riggs, J. M., Collins, P. L., Murphy, B. R. (2000). Replacement of the Ectodomains of the Hemagglutinin-Neuraminidase and Fusion Glycoproteins of Recombinant Parainfluenza Virus Type 3 (PIV3) with Their Counterparts from PIV2 Yields Attenuated PIV2 Vaccine Candidates. J. Virol. 74: 6448-6458 [Abstract] [Full Text]  
  • Schmitt, A. P., He, B., Lamb, R. A. (1999). Involvement of the Cytoplasmic Domain of the Hemagglutinin-Neuraminidase Protein in Assembly of the Paramyxovirus Simian Virus 5. J. Virol. 73: 8703-8712 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»