This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Blaney, J. E.
Right arrow Articles by Tevethia, S. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blaney, J. E., Jr.
Right arrow Articles by Tevethia, S. S.

 Previous Article  |  Next Article 

Journal of Virology, December 1998, p. 9567-9574, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Immunization with a Single Major Histocompatibility Complex Class I-Restricted Cytotoxic T-Lymphocyte Recognition Epitope of Herpes Simplex Virus Type 2 Confers Protective Immunity

Joseph E. Blaney Jr.,1 Eri Nobusawa,2,dagger Michael A. Brehm,1 Robert H. Bonneau,1 Lawrence M. Mylin,1 Tong-Ming Fu,1,Dagger Yoshihiro Kawaoka,2 and Satvir S. Tevethia1,*

Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033,1 and Department of Pathobiological Sciences, University of Wisconsin, Madison, Wisconsin 537062

Received 27 May 1998/Accepted 25 August 1998

We have evaluated the potential of conferring protective immunity to herpes simplex virus type 2 (HSV-2) by selectively inducing an HSV-specific CD8+ cytotoxic T-lymphocyte (CTL) response directed against a single major histocompatibility complex class I-restricted CTL recognition epitope. We generated a recombinant vaccinia virus (rVV-ES-gB498-505) which expresses the H-2Kb-restricted, HSV-1/2-cross-reactive CTL recognition epitope, HSV glycoprotein B residues 498 to 505 (SSIEFARL) (gB498-505), fused to the adenovirus type 5 E3/19K endoplasmic reticulum insertion sequence (ES). Mucosal immunization of C57BL/6 mice with this recombinant vaccinia virus induced both a primary CTL response in the draining lymph nodes and a splenic memory CTL response directed against HSV gB498-505. To determine the ability of the gB498-505-specific memory CTL response to provide protection from HSV infection, immunized mice were challenged with a lethal dose of HSV-2 strain 186 by the intranasal (i.n.) route. Development of the gB498-505-specific CTL response conferred resistance in 60 to 75% of mice challenged with a lethal dose of HSV-2 and significantly reduced the levels of infectious virus in the brains and trigeminal ganglia of challenged mice. Finally, i.n. immunization of C57BL/6 mice with either a recombinant influenza virus or a recombinant vaccinia virus expressing HSV gB498-505 without the ES was also demonstrated to induce an HSV-specific CTL response and provide protection from HSV infection. This finding confirms that the induction of an HSV-specific CTL response directed against a single epitope is sufficient for conferring protective immunity to HSV. Our findings support the role of CD8+ T cells in the control of HSV infection of the central nervous system and suggest the potential importance of eliciting HSV-specific mucosal CD8+ CTL in HSV vaccine design.

* Corresponding author. Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, 500 University Dr., Hershey, PA 17033. Phone: (717) 531-8872. Fax: (717) 531-6522. E-mail: sst1{at}psu.edu.

dagger Present address: Department of Virology, Nagoya City University Medical School, Mizuho-ku, Mizuho-cho, Nagoya, Japan.

Dagger Present address: Department of Virus and Cell Biology, Merck Research Laboratories, West Point, PA 19486.

Journal of Virology, December 1998, p. 9567-9574, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Muller, W. J., Dong, L., Vilalta, A., Byrd, B., Wilhelm, K. M., McClurkan, C. L., Margalith, M., Liu, C., Kaslow, D., Sidney, J., Sette, A., Koelle, D. M. (2009). Herpes simplex virus type 2 tegument proteins contain subdominant T-cell epitopes detectable in BALB/c mice after DNA immunization and infection. J. Gen. Virol. 90: 1153-1163 [Abstract] [Full Text]  
  • Zhang, X., Castelli, F. A., Zhu, X., Wu, M., Maillere, B., BenMohamed, L. (2008). Gender-Dependent HLA-DR-Restricted Epitopes Identified from Herpes Simplex Virus Type 1 Glycoprotein D. CVI 15: 1436-1449 [Abstract] [Full Text]  
  • de Witte, M. A., Bendle, G. M., van den Boom, M. D., Coccoris, M., Schell, T. D., Tevethia, S. S., van Tinteren, H., Mesman, E. M., Song, J.-Y., Schumacher, T. N. M. (2008). TCR Gene Therapy of Spontaneous Prostate Carcinoma Requires In Vivo T Cell Activation. J. Immunol. 181: 2563-2571 [Abstract] [Full Text]  
  • Koelle, D. M., Magaret, A., McClurkan, C. L., Remington, M. L., Warren, T., Teofilovici, F., Wald, A. (2008). Phase I Dose-Escalation Study of a Monovalent Heat Shock Protein 70-Herpes Simplex Virus Type 2 (HSV-2) Peptide-Based Vaccine Designed To Prime or Boost CD8 T-Cell Responses in HSV-Naive and HSV-2-Infected Subjects. CVI 15: 773-782 [Abstract] [Full Text]  
  • Lang, A., Brien, J. D., Messaoudi, I., Nikolich-Zugich, J. (2008). Age-Related Dysregulation of CD8+ T Cell Memory Specific for a Persistent Virus Is Independent of Viral Replication. J. Immunol. 180: 4848-4857 [Abstract] [Full Text]  
  • Kupresanin, F., Chow, J., Mount, A., Smith, C. M., Stevenson, P. G., Belz, G. T. (2007). Dendritic Cells Present Lytic Antigens and Maintain Function throughout Persistent {gamma}-Herpesvirus Infection. J. Immunol. 179: 7506-7513 [Abstract] [Full Text]  
  • Belz, G. T., Zhang, L., Lay, M. D. H., Kupresanin, F., Davenport, M. P. (2007). Killer T cells regulate antigen presentation for early expansion of memory, but not naive, CD8+ T cell. Proc. Natl. Acad. Sci. USA 104: 6341-6346 [Abstract] [Full Text]  
  • Zhang, X., Issagholian, A., Berg, E. A., Fishman, J. B., Nesburn, A. B., BenMohamed, L. (2005). Th-Cytotoxic T-Lymphocyte Chimeric Epitopes Extended by N{varepsilon}-Palmitoyl Lysines Induce Herpes Simplex Virus Type 1-Specific Effector CD8+ Tc1 Responses and Protect against Ocular Infection. J. Virol. 79: 15289-15301 [Abstract] [Full Text]  
  • van Lint, A. L., Kleinert, L., Clarke, S. R. M., Stock, A., Heath, W. R., Carbone, F. R. (2005). Latent Infection with Herpes Simplex Virus Is Associated with Ongoing CD8+ T-Cell Stimulation by Parenchymal Cells within Sensory Ganglia. J. Virol. 79: 14843-14851 [Abstract] [Full Text]  
  • Svensson, A., Kaim, J., Mallard, C., Olsson, A., Brodin, E., Hokfelt, T., Eriksson, K. (2005). Neurokinin 1 Receptor Signaling Affects the Local Innate Immune Defense against Genital Herpes Virus Infection. J. Immunol. 175: 6802-6811 [Abstract] [Full Text]  
  • Gherardi, M. M., Esteban, M. (2005). Recombinant poxviruses as mucosal vaccine vectors. J. Gen. Virol. 86: 2925-2936 [Abstract] [Full Text]  
  • Li, Z.-N., Mueller, S. N., Ye, L., Bu, Z., Yang, C., Ahmed, R., Steinhauer, D. A. (2005). Chimeric Influenza Virus Hemagglutinin Proteins Containing Large Domains of the Bacillus anthracis Protective Antigen: Protein Characterization, Incorporation into Infectious Influenza Viruses, and Antigenicity. J. Virol. 79: 10003-10012 [Abstract] [Full Text]  
  • Svensson, A., Nordstrom, I., Sun, J.-B., Eriksson, K. (2005). Protective Immunity to Genital Herpes Simpex Virus Type 2 Infection Is Mediated by T-bet. J. Immunol. 174: 6266-6273 [Abstract] [Full Text]  
  • Belz, G. T., Smith, C. M., Kleinert, L., Reading, P., Brooks, A., Shortman, K., Carbone, F. R., Heath, W. R. (2004). Distinct migrating and nonmigrating dendritic cell populations are involved in MHC class I-restricted antigen presentation after lung infection with virus. Proc. Natl. Acad. Sci. USA 101: 8670-8675 [Abstract] [Full Text]  
  • Schell, T. D. (2004). In Vivo Expansion of the Residual Tumor Antigen-Specific CD8+ T Lymphocytes That Survive Negative Selection in Simian Virus 40 T-Antigen-Transgenic Mice. J. Virol. 78: 1751-1762 [Abstract] [Full Text]  
  • Belz, G. T., Smith, C. M., Eichner, D., Shortman, K., Karupiah, G., Carbone, F. R., Heath, W. R. (2004). Cutting Edge: Conventional CD8{alpha}+ Dendritic Cells Are Generally Involved in Priming CTL Immunity to Viruses. J. Immunol. 172: 1996-2000 [Abstract] [Full Text]  
  • Suvas, S., Kumaraguru, U., Pack, C. D., Lee, S., Rouse, B. T. (2003). CD4+CD25+ T Cells Regulate Virus-specific Primary and Memory CD8+ T Cell Responses. JEM 198: 889-901 [Abstract] [Full Text]  
  • BenMohamed, L., Bertrand, G., McNamara, C. D., Gras-Masse, H., Hammer, J., Wechsler, S. L., Nesburn, A. B. (2003). Identification of Novel Immunodominant CD4+ Th1-Type T-Cell Peptide Epitopes from Herpes Simplex Virus Glycoprotein D That Confer Protective Immunity. J. Virol. 77: 9463-9473 [Abstract] [Full Text]  
  • Mueller, S. N., Jones, C. M., Chen, W., Kawaoka, Y., Castrucci, M. R., Heath, W. R., Carbone, F. R. (2003). The Early Expression of Glycoprotein B from Herpes Simplex Virus Can Be Detected by Antigen-Specific CD8+ T Cells. J. Virol. 77: 2445-2451 [Abstract] [Full Text]  
  • Koelle, D. M., Corey, L. (2003). Recent Progress in Herpes Simplex Virus Immunobiology and Vaccine Research. Clin. Microbiol. Rev. 16: 96-113 [Abstract] [Full Text]  
  • Gierynska, M., Kumaraguru, U., Eo, S.-K., Lee, S., Krieg, A., Rouse, B. T. (2002). Induction of CD8 T-Cell-Specific Systemic and Mucosal Immunity against Herpes Simplex Virus with CpG-Peptide Complexes. J. Virol. 76: 6568-6576 [Abstract] [Full Text]  
  • Jiang, S., Borthwick, N. J., Morrison, P., Gao, G. F., Steward, M. W. (2002). Virus-specific CTL responses induced by an H-2Kd-restricted, motif-negative 15-mer peptide from the fusion protein of respiratory syncytial virus. J. Gen. Virol. 83: 429-438 [Abstract] [Full Text]  
  • Wee, E. G.-T., Patel, S., McMichael, A. J., Hanke, T. (2002). A DNA/MVA-based candidate human immunodeficiency virus vaccine for Kenya induces multi-specific T cell responses in rhesus macaques. J. Gen. Virol. 83: 75-80 [Abstract] [Full Text]  
  • Kumaraguru, U., Gierynska, M., Norman, S., Bruce, B. D., Rouse, B. T. (2002). Immunization with Chaperone-Peptide Complex Induces Low-Avidity Cytotoxic T Lymphocytes Providing Transient Protection against Herpes Simplex Virus Infection. J. Virol. 76: 136-141 [Abstract] [Full Text]  
  • Koelle, D. M., Chen, H. B., Gavin, M. A., Wald, A., Kwok, W. W., Corey, L. (2001). CD8 CTL from Genital Herpes Simplex Lesions: Recognition of Viral Tegument and Immediate Early Proteins and Lysis of Infected Cutaneous Cells. J. Immunol. 166: 4049-4058 [Abstract] [Full Text]  
  • Mylin, L. M., Schell, T. D., Roberts, D., Epler, M., Boesteanu, A., Collins, E. J., Frelinger, J. A., Joyce, S., Tevethia, S. S. (2000). Quantitation of CD8+ T-Lymphocyte Responses to Multiple Epitopes from Simian Virus 40 (SV40) Large T Antigen in C57BL/6 Mice Immunized with SV40, SV40 T-Antigen-Transformed Cells, or Vaccinia Virus Recombinants Expressing Full-Length T Antigen or Epitope Minigenes. J. Virol. 74: 6922-6934 [Abstract] [Full Text]  
  • Kumaraguru, U., Rouse, B. T. (2000). Application of the Intracellular Gamma Interferon Assay To Recalculate the Potency of CD8+ T-Cell Responses to Herpes Simplex Virus. J. Virol. 74: 5709-5711 [Abstract] [Full Text]  
  • Schell, T. D., Knowles, B. B., Tevethia, S. S. (2000). Sequential Loss of Cytotoxic T Lymphocyte Responses to Simian Virus 40 Large T Antigen Epitopes in T Antigen Transgenic Mice Developing Osteosarcomas. Cancer Res. 60: 3002-3012 [Abstract] [Full Text]  
  • Schell, T. D., Mylin, L. M., Georgoff, I., Teresky, A. K., Levine, A. J., Tevethia, S. S. (1999). Cytotoxic T-Lymphocyte Epitope Immunodominance in the Control of Choroid Plexus Tumors in Simian Virus 40 Large T Antigen Transgenic Mice. J. Virol. 73: 5981-5993 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»