This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nédellec, P.
Right arrow Articles by Brahic, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nédellec, P.
Right arrow Articles by Brahic, M.

 Previous Article  |  Next Article 

Journal of Virology, December 1998, p. 9553-9560, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Interaction of Theiler's Virus with Intermediate Filaments of Infected Cells

Patrick Nédellec,1,dagger Patrick Vicart,2 Christine Laurent-Winter,3 Cécile Martinat,1 Marie-Christine Prévost,4 and Michel Brahic1,*

Unité des Virus Lents (ERS 572 CNRS),1 Station Centrale de Microscopie Electronique,2 Laboratoire d'Electrophorèse Bidimensionelle,3 and Laboratoire de Microscopie Electronique,4 Département SIDA & Rétrovirus, Institut Pasteur, 75724 Paris Cedex 15, France

Received 6 March 1998/Accepted 19 August 1998

Theiler's murine encephalomyelitis virus is a neurotropic murine picornavirus which replicates permissively and causes a cytopathic effect in the BHK-21 cell line. We examined the interactions between the GDVII and DA strains of Theiler's virus and BHK-21 host cell proteins in a virus overlay assay. We observed binding of the virions to two proteins of approximately 60 kDa. These proteins were microsequenced and identified as desmin and vimentin, two main components of the intermediate filament network. The association between desmin or vimentin and virions was demonstrated by immunoprecipitation. Anti-desmin and anti-vimentin monoclonal antibodies precipitated GDVII or DA virions from extracts of infected BHK-21 cells. The intracellular distributions of virions and of the desmin and vimentin intermediate filaments of BHK-21 cells were investigated by two-color immunofluorescence confocal microscopy. Following infection, the intermediate filament network was rearranged into a shell-like structure which surrounded a viral inclusion. Finally, close contact between GDVII virus particles and 10-nm intermediate filaments was observed by electron microscopy.

* Corresponding author. Mailing address: Unité des Virus Lents, ERS 572 CNRS, Institut Pasteur, 75724 Paris Cedex 15, France. Phone: 33 1 45 68 87 70. Fax: 33 1 40 61 31 67. E-mail: mbrahic{at}pasteur.fr.

dagger Present address: Laboratoire de Neurobiologie Cellulaire et Moléculaire, EP 1591 CNRS, Faculté des Sciences Pharmaceutiques et Biologiques, 75006 Paris, France.

Journal of Virology, December 1998, p. 9553-9560, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Miller, M. S., Hertel, L. (2009). Onset of Human Cytomegalovirus Replication in Fibroblasts Requires the Presence of an Intact Vimentin Cytoskeleton. J. Virol. 83: 7015-7028 [Abstract] [Full Text]  
  • Armer, H., Moffat, K., Wileman, T., Belsham, G. J., Jackson, T., Duprex, W. P., Ryan, M., Monaghan, P. (2008). Foot-and-Mouth Disease Virus, but Not Bovine Enterovirus, Targets the Host Cell Cytoskeleton via the Nonstructural Protein 3Cpro. J. Virol. 82: 10556-10566 [Abstract] [Full Text]  
  • Kirilyuk, A., Tolstonog, G. V., Damert, A., Held, U., Hahn, S., Lower, R., Buschmann, C., Horn, A. V., Traub, P., Schumann, G. G. (2008). Functional endogenous LINE-1 retrotransposons are expressed and mobilized in rat chloroleukemia cells. Nucleic Acids Res 36: 648-665 [Abstract] [Full Text]  
  • Ramanathan, H. N., Chung, D.-H., Plane, S. J., Sztul, E., Chu, Y.-k., Guttieri, M. C., McDowell, M., Ali, G., Jonsson, C. B. (2007). Dynein-Dependent Transport of the Hantaan Virus Nucleocapsid Protein to the Endoplasmic Reticulum-Golgi Intermediate Compartment. J. Virol. 81: 8634-8647 [Abstract] [Full Text]  
  • Kim, J.-K., Fahad, A.-M., Shanmukhappa, K., Kapil, S. (2006). Defining the Cellular Target(s) of Porcine Reproductive and Respiratory Syndrome Virus Blocking Monoclonal Antibody 7G10. J. Virol. 80: 689-696 [Abstract] [Full Text]  
  • Stefanovic, S., Windsor, M., Nagata, K.-i., Inagaki, M., Wileman, T. (2005). Vimentin Rearrangement during African Swine Fever Virus Infection Involves Retrograde Transport along Microtubules and Phosphorylation of Vimentin by Calcium Calmodulin Kinase II. J. Virol. 79: 11766-11775 [Abstract] [Full Text]  
  • Ritzenthaler, C., Laporte, C., Gaire, F., Dunoyer, P., Schmitt, C., Duval, S., Piequet, A., Loudes, A. M., Rohfritsch, O., Stussi-Garaud, C., Pfeiffer, P. (2002). Grapevine Fanleaf Virus Replication Occurs on Endoplasmic Reticulum-Derived Membranes. J. Virol. 76: 8808-8819 [Abstract] [Full Text]  
  • Risco, C., Rodriguez, J. R., Lopez-Iglesias, C., Carrascosa, J. L., Esteban, M., Rodriguez, D. (2002). Endoplasmic Reticulum-Golgi Intermediate Compartment Membranes and Vimentin Filaments Participate in Vaccinia Virus Assembly. J. Virol. 76: 1839-1855 [Abstract] [Full Text]  
  • Heath, C. M., Windsor, M., Wileman, T. (2001). Aggresomes Resemble Sites Specialized for Virus Assembly. JCB 153: 449-456 [Abstract] [Full Text]  
  • Henzler, T., Harmache, A., Herrmann, H., Spring, H., Suzan, M., Audoly, G., Panek, T., Bosch, V. (2001). Fully functional, naturally occurring and C-terminally truncated variant human immunodeficiency virus (HIV) Vif does not bind to HIV Gag but influences intermediate filament structure. J. Gen. Virol. 82: 561-573 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»