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Journal of Virology, December 1998, p. 10265-10269, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Alphavirus Replicase Protein NSP1 Induces Filopodia and Rearrangement of Actin Filaments

Pirjo Laakkonen, Petri Auvinen, Pekka Kujala, and Leevi Kääriäinen*

Institute of Biotechnology, Viikki Biocenter, University of Helsinki, Helsinki, Finland

Received 18 May 1998/Accepted 25 August 1998

Expression of the NSP1 protein of Semliki Forest virus and Sindbis virus in cultured cells induced filopodia-like extensions containing NSP1 but not F actin. The actin stress fibers disappeared, whereas vimentin, keratin, and tubulin networks remained intact. The effects of NSP1 were dependent on its palmitoylation but not on its enzymatic activities and were also observed in virus-infected cells.

* Corresponding author. Mailing address: Institute of Biotechnology, P.O. Box 56, Viikinkaari 9, 00014 University of Helsinki, Helsinki, Finland. Phone: 358-9-70859400. Fax: 358-9-70859560. E-mail: leevi.kaariainen{at}helsinki.fi.

Journal of Virology, December 1998, p. 10265-10269, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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