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Journal of Virology, December 1998, p. 10260-10264, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Construction and Characterization of Hexon-Chimeric Adenoviruses: Specification of Adenovirus Serotype

Jason G. D. Gall,1,dagger Ronald G. Crystal,2 and Erik Falck-Pedersen1,*

Department of Microbiology, W. R. Hearst Research Foundation,1 and Department of Medicine,2 Cornell University Medical College, New York, New York 10021

Received 22 June 1998/Accepted 9 September 1998

This study has used the strategy of gene replacement to characterize the contribution of the adenovirus (Ad) capsid protein hexon to serotype definition. By replacing the Ad type 5 (Ad5) hexon gene with sequences from Ad2, we have changed the type specificity of the chimeric virus. The type-determining epitopes are primarily associated with loop 1 of hexon and, to a much lesser degree, with loop 2. In spite of the serotype distinctiveness of the chimeric hexon viruses, epitope similarity between the vectors resulted in a low level of cross-reactive neutralizing antibody, which in combination with activated cellular and innate arms of the immune system is sufficient to suppress gene transduction following readministration in vivo.

* Corresponding author. Mailing address: Department of Microbiology, Box 62, Cornell University Medical College, 1300 York Ave., New York, NY 10021. Phone: (212) 746-6514. Fax: (212) 746-8587. E-mail: efalckp{at}mail.med.cornell.edu.

dagger Present address: Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, CA 92037.

Journal of Virology, December 1998, p. 10260-10264, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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