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Journal of Virology, December 1998, p. 10260-10264, Vol. 72, No. 12
Department of Microbiology,
Received 22 June 1998/Accepted 9 September 1998
This study has used the strategy of gene replacement to
characterize the contribution of the adenovirus (Ad) capsid protein hexon to serotype definition. By replacing the Ad type 5 (Ad5) hexon
gene with sequences from Ad2, we have changed the type specificity of
the chimeric virus. The type-determining epitopes are primarily associated with loop 1 of hexon and, to a much lesser degree, with loop
2. In spite of the serotype distinctiveness of the chimeric hexon
viruses, epitope similarity between the vectors resulted in a low level
of cross-reactive neutralizing antibody, which in combination with
activated cellular and innate arms of the immune system is sufficient
to suppress gene transduction following readministration in vivo.
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Construction and Characterization of Hexon-Chimeric
Adenoviruses: Specification of Adenovirus Serotype

*
Corresponding author. Mailing address: Department of
Microbiology, Box 62, Cornell University Medical College, 1300 York
Ave., New York, NY 10021. Phone: (212) 746-6514. Fax: (212) 746-8587. E-mail: efalckp{at}mail.med.cornell.edu.
Present address: Laboratory of Genetics, The Salk Institute for
Biological Studies, La Jolla, CA 92037.
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