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Journal of Virology, December 1998, p. 10242-10245, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Retrovirus Packaging Cells Expressing the Mus dunni Endogenous Virus Envelope Facilitate Transduction of CHO and Primary Hematopoietic Cells

Greg Wolgamot,1,2,3 John E. J. Rasko,1 and A. Dusty Miller1,2,*

Fred Hutchinson Cancer Research Center, Seattle, Washington 98109,1 and Department of Pathology2 and Medical Scientist Training Program,3 University of Washington, Seattle, Washington 98195

Received 6 July 1998/Accepted 7 September 1998

Mus dunni endogenous virus (MDEV) infects a wide variety of cell types from many different species. To take advantage of this broad host range, we have constructed packaging cells (PD223) that produce virions bearing the MDEV envelope. PD223 cells are able to package Moloney murine leukemia virus-based vectors at a titer of 4 × 105 infectious units per ml in the absence of contaminating replication-competent retrovirus. Vectors packaged by PD223 cells are able to transduce CHO cells, which are resistant to transduction by many retroviruses, at >= 25-fold higher efficiency than vectors having other pseudotypes. A vector packaged by PD223 was found to be among the most efficient for transducing primary baboon and human CD34+ cells.

* Corresponding author. Mailing address: Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. North, Seattle, WA 98109-1024. Phone: (206) 667-2890. Fax: (206) 667-6523. E-mail: dmiller{at}fhcrc.org.

Journal of Virology, December 1998, p. 10242-10245, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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  • Rasko, J. E. J., Battini, J.-L., Gottschalk, R. J., Mazo, I., Miller, A. D. (1999). The RD114/simian type D retrovirus receptor is a neutral amino acid transporter. Proc. Natl. Acad. Sci. USA 96: 2129-2134 [Abstract] [Full Text]  


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