This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thomas, C. J.
Right arrow Articles by Possee, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thomas, C. J.
Right arrow Articles by Possee, R. D.

 Previous Article  |  Next Article 

Journal of Virology, December 1998, p. 10207-10212, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Localization of a Baculovirus-Induced Chitinase in the Insect Cell Endoplasmic Reticulum

Carole J. Thomas,1,2 Helen L. Brown,2 Chris R. Hawes,2 Bum Yong Lee,3 Mi-Kyung Min,3 Linda A. King,2 and Robert D. Possee1,*

NERC Institute of Virology and Environmental Microbiology, Oxford OX1 3SR,1 and School of Biological and Molecular Sciences, Oxford Brookes University, Oxford OX3 0BP,2 United Kingdom, and MOGAM Biotechnology Research Institute, Yongin-Kun, Kyonggi-Do 449-910, Korea3

Received 17 March 1998/Accepted 10 September 1998

Confocal immunofluorescence microscopy was used to demonstrate that the Autographa californica nucleopolyhedrovirus (AcMNPV) chitinase was localized within the endoplasmic reticulum (ER) of virus-infected insect cells. This was consistent with removal of the signal peptide from the chitinase and an ER localization motif (KDEL) at the carboxyl end of the protein. Chitinase release from cells, a prerequisite for liquefaction of virus-infected insect larvae, appears to be aided by synthesis of the p10 protein. Deletion of p10 from the AcMNPV genome delayed the appearance of chitinase activity in the medium of virus-infected cells by 24 h and also delayed liquefaction of virus-infected Trichoplusia ni larvae by the same period.

* Corresponding author. Mailing address: NERC Institute of Virology and Environmental Microbiology, Mansfield Rd., Oxford OX1 3SR, United Kingdom. Phone: 44 1865 281663. Fax: 44 1865 281696. E-mail: rpossee{at}worf.molbiol.ox.ac.uk.

Journal of Virology, December 1998, p. 10207-10212, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hodgson, J. J., Arif, B. M., Krell, P. J. (2009). Autographa californica multiple nucleopolyhedrovirus and Choristoneura fumiferana multiple nucleopolyhedrovirus v-cath genes are expressed as pre-proenzymes. J. Gen. Virol. 90: 995-1000 [Abstract] [Full Text]  
  • Hodgson, J. J., Arif, B. M., Krell, P. J. (2007). Reprogramming the chiA expression profile of Autographa californica multiple nucleopolyhedrovirus. J. Gen. Virol. 88: 2479-2487 [Abstract] [Full Text]  
  • Young, V. L., Simpson, R. M., Ward, V. K. (2005). Characterization of an exochitinase from Epiphyas postvittana nucleopolyhedrovirus (family Baculoviridae). J. Gen. Virol. 86: 3253-3261 [Abstract] [Full Text]  
  • Saville, G. P., Patmanidi, A. L., Possee, R. D., King, L. A. (2004). Deletion of the Autographa californica nucleopolyhedrovirus chitinase KDEL motif and in vitro and in vivo analysis of the modified virus. J. Gen. Virol. 85: 821-831 [Abstract] [Full Text]  
  • Hagiwara, K., Rao, S., Scott, S. W., Carner, G. R. (2002). Nucleotide sequences of segments 1, 3 and 4 of the genome of Bombyx mori cypovirus 1 encoding putative capsid proteins VP1, VP3 and VP4, respectively. J. Gen. Virol. 83: 1477-1482 [Abstract] [Full Text]  
  • Saville, G. P., Thomas, C. J., Possee, R. D., King, L. A. (2002). Partial redistribution of the Autographa californica nucleopolyhedrovirus chitinase in virus-infected cells accompanies mutation of the carboxy-terminal KDEL ER-retention motif. J. Gen. Virol. 83: 685-694 [Abstract] [Full Text]  
  • Ko, R., Okano, K., Maeda, S. (2000). Structural and Functional Analysis of the Xestia c-nigrum Granulovirus Matrix Metalloproteinase. J. Virol. 74: 11240-11246 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»