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Journal of Virology, December 1998, p. 10180-10188, Vol. 72, No. 12
AIDS Pathogenesis Research Unit,
Received 29 May 1998/Accepted 20 August 1998
The induction of human immunodeficiency virus (HIV)-specific T-cell
responses is widely seen as critical to the development of effective
immunity to HIV type 1 (HIV-1). Plasmid DNA and recombinant fowlpox
virus (rFPV) vaccines are among the most promising safe HIV-1 vaccine
candidates. However, the immunity induced by either vaccine alone may
be insufficient to provide durable protection against HIV-1 infection.
We evaluated a consecutive immunization strategy involving priming with
DNA and boosting with rFPV vaccines encoding common HIV-1 antigens. In
mice, this approach induced greater HIV-1-specific immunity than either
vector alone and protected mice from challenge with a recombinant
vaccinia virus expressing HIV-1 antigens. In macaques, a dramatic
boosting effect on DNA vaccine-primed HIV-1-specific helper and
cytotoxic T-lymphocyte responses, but a decline in HIV-1 antibody
titers, was observed following rFPV immunization. The vaccine regimen
protected macaques from an intravenous HIV-1 challenge, with the
resistance most likely mediated by T-cell responses. These studies
suggest a safe strategy for the enhanced generation of T-cell-mediated
protective immunity to HIV-1.
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Enhanced T-Cell Immunogenicity and Protective Efficacy of a Human
Immunodeficiency Virus Type 1 Vaccine Regimen Consisting of
Consecutive Priming with DNA and Boosting with Recombinant
Fowlpox Virus
*
Corresponding author. Mailing address: Macfarlane
Burnet Centre for Medical Research, P. O. Box 254, Fairfield, Vic,
3078, Australia. Phone: 61392822175. Fax: 61394826152. E-mail:
kent{at}burnet.edu.au.
Journal of Virology, December 1998, p. 10180-10188, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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