Previous Article | Next Article ![]()
Journal of Virology, December 1998, p. 10148-10156, Vol. 72, No. 12
Division of Infectious Diseases, Thomas
Jefferson University, Philadelphia, Pennsylvania
19107,1 and
Paul Ehrlich Institut,
63225 Langen, Germany2
Received 20 April 1998/Accepted 10 September 1998
The successful application of human gene therapy protocols on a
broad clinical basis will depend on the availability of in vivo
cell-type-specific gene delivery systems. We have developed retroviral
vector particles, derived from spleen necrosis virus (SNV), that
display the antigen binding site of an antibody on the viral surface.
Using retroviral vectors derived from SNV that displayed single-chain
antibodies (scAs) directed against a carcinoembryonic antigen-cross-reacting cell surface protein, we have shown that an
efficient, cell-type-specific gene delivery can be obtained. In this
study, we tested whether other scAs displayed on SNV vector particles
can also lead to cell-type-specific gene delivery. We displayed the
following scAs on the retroviral surface: one directed against the
human cell surface antigen Her2neu, which belongs to the epidermal
growth factor receptor family; one directed against the stem
cell-specific antigen CD34; and one directed against the transferrin
receptor, which is expressed on liver cells and various other tissues.
We show that retroviral vectors displaying these scAs are competent for
infection in human cells which express the antigen recognized by the
scA. Infectivity was cell type specific, and titers above
105 CFU per ml of tissue culture supernatant medium were
obtained. The density of the antigen on the target cell surface does
not influence virus titers in vitro. Our data indicate that the SNV vector system is well suited for the development of a large variety of
cell-type-specific targeting vectors.
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Cell-Type-Specific Gene Transfer into Human Cells
with Retroviral Vectors That Display Single-Chain Antibodies

*
Corresponding author. Mailing address: Division of
Infectious Diseases, Thomas Jefferson University, Jefferson Alumni
Hall, 1020 Locust St., Suite 329, Philadelphia, PA 19107. Phone: (215) 503-3117. Fax: (215) 923-1956. E-mail:
rpomvicl{at}jeflin.tju.edu.
Present address: New York Blood Center, Department of
Immunochemistry, New York, NY 10021.
This article has been cited by other articles:
| J. Bacteriol. | Mol. Cell. Biol. | Microbiol. Mol. Biol. Rev. |
|---|
| Clin. Vaccine Immunol. | ALL ASM JOURNALS |
|---|