This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Iwata, Y.
Right arrow Articles by Shigeta, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Iwata, Y.
Right arrow Articles by Shigeta, S.

 Previous Article  |  Next Article 

Journal of Virology, December 1998, p. 10044-10049, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection and Sequence Analysis of Borna Disease Virus p24 RNA from Peripheral Blood Mononuclear Cells of Patients with Mood Disorders or Schizophrenia and of Blood Donors

Yasuhide Iwata,1,dagger Kazuo Takahashi,1,* Xie Peng,2,Dagger Koji Fukuda,2 Koei Ohno,1 Tsuguhiro Ogawa,1 Kenji Gonda,1 Norio Mori,2 Shin-ichi Niwa,3 and Shiro Shigeta1

Department of Microbiology1 and Department of Neuropsychiatry,3 School of Medicine, Fukushima Medical University, Fukushima-shi, Fukushima, 960-1295, and Department of Psychiatry and Neurology, Hamamatsu University School of Medicine, Hamamatsu-shi, Shizuoka, 431-3192,2 Japan

Received 27 April 1998/Accepted 24 August 1998

Borna disease virus (BDV) p24 RNA was detected in the peripheral blood mononuclear cells (PBMCs) of psychiatric patients and blood donors by nested reverse transcriptase PCR (RT-PCR). The prevalences of BDV p24 RNA in patients with mood disorders (4%) and schizophrenia (4%) were not significantly different from that in blood donors (2%). This finding was inconsistent with previous reports that showed either a high prevalence or absence of BDV p24 RNA in patients with psychiatric disorders. The differences in BDV p24 RNA prevalence in these studies may be due to differences in the criteria for positivity, the number of PBMCs used for RNA extraction, or the amount of RNA tested for nested RT-PCR or to laboratory contamination. Sequence analysis of BDV p24 RNA from the PBMCs of patients and blood donors showed a high nucleotide sequence conservation but definite nucleotide mutations compared with horse BDV p24 RNA sequences. In comparison with human BDV p24 RNA sequences previously reported from Japan and Germany, there were several positions with silent nucleotide mutations among these clones.

* Corresponding author. Mailing address: Department of Microbiology, School of Medicine, Fukushima Medical University, 1 Hikarigaoka, Fukushima-shi, Fukushima, 960-1295, Japan. Phone: 81-24-548-2111, ext. 2162. Fax: 81-24-548-5072. E-mail: k-tak{at}cc.fmu.ac.jp.

dagger Present address: Department of Psychiatry and Neurology, Hamamatsu University School of Medicine, Hamamatsu-shi, Shizuoka, 431-3192, Japan.

Dagger Present address: Department of Neurology and Psychiatry, First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, People's Republic of China.

Journal of Virology, December 1998, p. 10044-10049, Vol. 72, No. 12
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Chalmers, R.M., Thomas, D.Rh., Salmon, R.L. (2005). Borna disease virus and the evidence for human pathogenicity: a systematic review. QJM 98: 255-274 [Abstract] [Full Text]  
  • Bode, L., Ludwig, H. (2003). Borna Disease Virus Infection, a Human Mental-Health Risk. Clin. Microbiol. Rev. 16: 534-545 [Abstract] [Full Text]  
  • Fukuda, K., Takahashi, K., Iwata, Y., Mori, N., Gonda, K., Ogawa, T., Osonoe, K., Sato, M., Ogata, S.-i., Horimoto, T., Sawada, T., Tashiro, M., Yamaguchi, K., Niwa, S.-i., Shigeta, S. (2001). Immunological and PCR Analyses for Borna Disease Virus in Psychiatric Patients and Blood Donors in Japan. J. Clin. Microbiol. 39: 419-429 [Abstract] [Full Text]  
  • Staeheli, P., Sauder, C., Hausmann, J., Ehrensperger, F., Schwemmle, M. (2000). Epidemiology of Borna disease virus. J. Gen. Virol. 81: 2123-2135 [Full Text]  
  • Formella, S., Jehle, C., Sauder, C., Staeheli, P., Schwemmle, M. (2000). Sequence Variability of Borna Disease Virus: Resistance to Superinfection May Contribute to High Genome Stability in Persistently Infected Cells. J. Virol. 74: 7878-7883 [Abstract] [Full Text]  
  • Nowotny, N., Kolodziejek, J., Jehle, C. O., Suchy, A., Staeheli, P., Schwemmle, M. (2000). Isolation and Characterization of a New Subtype of Borna Disease Virus. J. Virol. 74: 5655-5658 [Abstract] [Full Text]  
  • Hausmann, J., Hallensleben, W., de la Torre, J. C., Pagenstecher, A., Zimmermann, C., Pircher, H., Staeheli, P. (1999). T cell ignorance in mice to Borna disease virus can be overcome by peripheral expression of the viral nucleoprotein. Proc. Natl. Acad. Sci. USA 96: 9769-9774 [Abstract] [Full Text]  
  • Planz, O., Rentzsch, C., Batra, A., Batra, A., Winkler, T., Büttner, M., Rziha, H.-J., Stitz, L. (1999). Pathogenesis of Borna Disease Virus: Granulocyte Fractions of Psychiatric Patients Harbor Infectious Virus in the Absence of Antiviral Antibodies. J. Virol. 73: 6251-6256 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»