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Journal of Virology, November 1998, p. 9348-9352, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Attachment and Growth of Human Rotaviruses RV-3 and S12/85 in Caco-2 Cells Depend on VP4

Carl D. Kirkwood,1,dagger Ruth F. Bishop,1 and Barbara S. Coulson2,*

Department of Gastroenterology and Clinical Nutrition, Royal Children's Hospital,1 and Department of Microbiology and Immunology, The University of Melbourne,2 Parkville 3052, Victoria, Australia

Received 4 May 1998/Accepted 24 July 1998

Studies with human neonatal rotaviruses RV-3 and S12/85 and their reassortants showed that VP4 is a determinant of rotavirus attachment to and growth in Caco-2 cells. The binding of these viruses to MA104 and Caco-2 cells correlated with their growth ability. Virus sensitivity to trypsin and the VP4 fusion region may be implicated in these processes.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, The University of Melbourne, Royal Parade, Parkville 3052, Victoria, Australia. Phone: 61 3 9344 8823. Fax: 61 3 9347 1540. E-mail: b.coulson{at}microbiology.unimelb.edu.au.

dagger Present address: Viral Gastroenteritis Section, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333.

Journal of Virology, November 1998, p. 9348-9352, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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