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Journal of Virology, November 1998, p. 9323-9328, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Epstein-Barr Virus Small RNA (EBER) Genes: Differential Regulation during Lytic Viral Replication

Norbert Greifenegger,1 Michael Jäger,1 Leoni A. Kunz-Schughart,2 Hans Wolf,1 and Fritz Schwarzmann1,*

Institut für Medizinische Mikrobiologie und Hygiene1 and Institut für Pathologie,2 Universität Regensburg, Regensburg, Germany

Received 1 May 1998/Accepted 11 August 1998

In every latently Epstein-Barr virus-infected cell the viral genes EBER-1 and EBER-2 are transcribed by polymerase III. In lytically infected cells in vivo the EBER genes could not be detected. However, in cell culture downregulation could not be confirmed, and hence the relevance of this shutdown to the replication of the virus was not clear. We assayed the transcriptional activity of the EBER genes by nuclear run-on assays with enriched lytically infected cells and demonstrated that EBER-1 and EBER-2 are differentially downregulated on the transcriptional level during the switch to lytic viral replication. This downregulation was an early event during the lytic replication of the virus.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Franz-Josef-Strauss-Allee 11, D-93053 Regensburg, Germany. Phone: 49 941 9446452. Fax: 49 941 944 6402. E-mail: Fritz.Schwarzmann{at}klinik.uni-regensburg.de.

Journal of Virology, November 1998, p. 9323-9328, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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