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Journal of Virology, November 1998, p. 9278-9285, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Transcriptional Organization of the Avian Adenovirus CELO

Vincent Payet, Claire Arnauld, Jean-Paul Picault, André Jestin, and Patrick Langlois*

Unité de Biologie Moleculaire, Centre National d'Etudes Vétérinaires et Alimentaires, 22440 Ploufragan, France

Received 2 April 1998/Accepted 10 July 1998

A detailed map of the transcriptional organization of the CELO virus genome was produced. Recent computer analysis of CELO virus has indicated the presence of 38 putative open reading frames (ORFs). This study, based on analysis of the transcriptional products of CELO in vitro, confirmed the presence of RNAs for 26 of these 38 ORFs. All of the results were obtained by cDNA isolation or specific reverse transcriptase PCR. Observation of ORF transcription kinetics postinfection revealed the existence of early and late expression, with the earliest starting at 2 h postinfection. The 5' untranslated regions of some RNAs were also studied, and this revealed the existence of a bipartite leader sequence, comparable in structure to the tripartite leader of mastadenovirus. The leader most probably involved in transcriptional activity was observed in most of the structural protein genes of the CELO virus genome. This suggests some homology in transcriptional organization between the avian adenovirus CELO and known mastadenoviruses such as human adenovirus.

* Corresponding author. Mailing address: Centre National d'Etudes Vétérinaires et Alimentaires, Unité de Biologie Moleculaire, BP53, 22440 Ploufragan, France. Phone: 33-02-96-76-01-16. Fax: 33-02-96-76-01-23. E-mail: p.langlois{at}ploufragan.cneva.fr.

Journal of Virology, November 1998, p. 9278-9285, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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