This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Qin, S.
Right arrow Articles by Lazarowitz, S. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qin, S.
Right arrow Articles by Lazarowitz, S. G.

 Previous Article  |  Next Article 

Journal of Virology, November 1998, p. 9247-9256, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Bipartite Geminivirus Coat Protein Aids BR1 Function in Viral Movement by Affecting the Accumulation of Viral Single-Stranded DNA

Shenwei Qin,dagger Brian M. Ward,Dagger and Sondra G. Lazarowitz*

Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801

Received 16 December 1996/Accepted 18 July 1998

The movement of bipartite geminiviruses such as squash leaf curl virus (SqLCV) requires the cooperative interaction of two essential virus-encoded movement proteins, BR1 and BL1. While the viral coat protein AR1 is not essential for systemic infection, genetic studies demonstrate that its presence masks the defective phenotype of certain BR1 missense mutants, thus suggesting that coat protein does interact with the viral movement pathway. To further examine the mechanism of this interaction, we have constructed alanine-scanning mutants of AR1 and studied them for the ability to mask the infectivity defects of appropriate BR1 mutants, for the ability to target to the nucleus and to bind viral single-stranded DNA (ssDNA) and multimerize, and for effects on the accumulation of replicated viral ssDNA. We identified a specific region of AR1 required for masking of appropriate BR1 mutants and showed that this same region of AR1 was also important for ssDNA binding and the accumulation of viral replicated ssDNA. This region of AR1 also overlapped that involved in multimerization of the coat protein. We also found that the accumulation in protoplasts of single-stranded forms of a recombinant plasmid that included the SqLCV replication origin but was too large to be encapsidated was dependent on the presence of AR1 but did not appear to require encapsidation. These findings extend our model for SqLCV movement, demonstrating that coat protein affects viral movement through its ability to induce the accumulation of replicated viral ssDNA genomes. They further suggested that encapsidation was not required for the AR1-dependent accumulation of viral ssDNA.

* Corresponding author. Present address: Department of Plant Pathology, Cornell University, Ithaca, NY 14853. Phone: (607) 255-7830. Fax: (607) 255-4471. E-mail: sgl5{at}cornell.edu.

dagger Present address: Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032.

Dagger Present address: Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.

Journal of Virology, November 1998, p. 9247-9256, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Idris, A. M., Mills-Lujan, K., Martin, K., Brown, J. K. (2008). Melon Chlorotic Leaf Curl Virus: Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus. J. Virol. 82: 1959-1967 [Abstract] [Full Text]  
  • Timmusk, S., Fossum, C., Berg, M. (2006). Porcine circovirus type 2 replicase binds the capsid protein and an intermediate filament-like protein.. J. Gen. Virol. 87: 3215-3223 [Abstract] [Full Text]  
  • Heath, L., Williamson, A.-L., Rybicki, E. P. (2006). The Capsid Protein of Beak and Feather Disease Virus Binds to the Viral DNA and Is Responsible for Transporting the Replication-Associated Protein into the Nucleus. J. Virol. 80: 7219-7225 [Abstract] [Full Text]  
  • Carvalho, M. F., Turgeon, R., Lazarowitz, S. G. (2006). The Geminivirus Nuclear Shuttle Protein NSP Inhibits the Activity of AtNSI, a Vascular-Expressed Arabidopsis Acetyltransferase Regulated with the Sink-to-Source Transition. Plant Physiol. 140: 1317-1330 [Abstract] [Full Text]  
  • Carvalho, M. F., Lazarowitz, S. G. (2004). Interaction of the Movement Protein NSP and the Arabidopsis Acetyltransferase AtNSI Is Necessary for Cabbage Leaf Curl Geminivirus Infection and Pathogenicity. J. Virol. 78: 11161-11171 [Abstract] [Full Text]  
  • Hehnle, S., Wege, C., Jeske, H. (2004). Interaction of DNA with the Movement Proteins of Geminiviruses Revisited. J. Virol. 78: 7698-7706 [Abstract] [Full Text]  
  • Raghavan, V., Malik, P. S., Choudhury, N. R., Mukherjee, S. K. (2004). The DNA-A Component of a Plant Geminivirus (Indian Mung Bean Yellow Mosaic Virus) Replicates in Budding Yeast Cells. J. Virol. 78: 2405-2413 [Abstract] [Full Text]  
  • Hefferon, K. L., Dugdale, B. (2003). Independent expression of Rep and RepA and their roles in regulating bean yellow dwarf virus replication. J. Gen. Virol. 84: 3465-3472 [Abstract] [Full Text]  
  • McGarry, R. C., Barron, Y. D., Carvalho, M. F., Hill, J. E., Gold, D., Cheung, E., Kraus, W. L., Lazarowitz, S. G. (2003). A Novel Arabidopsis Acetyltransferase Interacts with the Geminivirus Movement Protein NSP. Plant Cell 15: 1605-1618 [Abstract] [Full Text]  
  • Liu, H., Boulton, M. I., Oparka, K. J., Davies, J. W. (2001). Interaction of the movement and coat proteins of Maize streak virus: implications for the transport of viral DNA. J. Gen. Virol. 82: 35-44 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»