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Journal of Virology, November 1998, p. 9092-9100, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Studies of the Neutralizing Activity and Avidity of
Anti-Human Immunodeficiency Virus Type 1 Env Antibody Elicited by
DNA Priming and Protein Boosting
J. F. L.
Richmond,1
S.
Lu,2
J. C.
Santoro,1
J.
Weng,1
Shiu-Lok
Hu,3
D. C.
Montefiori,4 and
H. L.
Robinson1,*
Department of
Pathology1 and
Department of Infectious
Diseases,2 University of Massachusetts School of
Medicine, Worcester, Massachusetts;
Washington Regional Primate
Research Center, University of Washington, Seattle,
Washington3; and
Department of Surgery
and Center for AIDS Research, Duke University Medical Center,
Durham, North Carolina4
Received 11 May 1998/Accepted 10 August 1998
DNA vaccination is an effective means of eliciting strong antibody
responses to a number of viral antigens. However, DNA immunization alone has not generated persistent, high-titer antibody and
neutralizing antibody responses to human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env). We have previously reported that DNA-primed anti-Env antibody responses can be augmented by boosting with Env-expressing recombinant vaccinia viruses. We report here that
recombinant Env protein provides a more effective boost of DNA-initiated antibody responses. In rabbits primed with Env-expressing plasmids, protein boosting increased titer, persistence, neutralizing activity, and avidity of anti-Env responses. While titers increased rapidly after boosting, avidity and neutralizing activity matured more
slowly over a 6-month period following protein boosting. DNA priming
and protein immunization with HIV-1 HXB-2 Env elicited neutralizing
antibody for T cell line-adapted, but not primary isolate, viruses. The
most effective neutralizing antibody responses were observed after
priming with plasmids which expressed noninfectious virus-like
particles. In contrast to immunizations with HIV-1 Env, DNA
immunizations with the influenza virus hemagglutinin glycoprotein did
not require a protein boost to achieve high-titer antibody with good
avidity and persistence.
*
Corresponding author. Present address: Division of
Microbiology and Immunology, Yerkes Primate Research Center, 954 Gatewood NE, Atlanta, GA 30329. Phone: (404) 727-7217. Fax: (404)
727-7768. E-mail: hrobins{at}rmy.emory.edu.
Journal of Virology, November 1998, p. 9092-9100, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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