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Journal of Virology, November 1998, p. 8669-8675, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Interactions of Soluble Recombinant Integrin alpha vbeta 5 with Human Adenoviruses

Patricia Mathias,1 Michael Galleno,2 and Glen R. Nemerow1,*

The Scripps Research Institute, La Jolla,1 and Invitrogen, Carlsbad,2 California

Received 11 June 1998/Accepted 7 August 1998

alpha v integrins have been identified as coreceptors for adenovirus (Ad) internalization; however, direct interactions of these molecules with Ad have not been demonstrated. We report here the expression of soluble integrin alpha vbeta 5, which retains the ability to recognize the Ad penton base as well as vitronectin, an Arg Gly Asp (RGD)-containing extracellular matrix protein. Soluble integrin alpha vbeta 5 reacted with seven different Ad serotypes (subgroups A to E) in solid-phase binding assays. The soluble integrin exhibited different levels of binding to each Ad serotype; however, binding to multiple Ad types required the presence of divalent metal cations and was inhibited by a synthetic RGD peptide, indicating that RGD and cation-binding sequences regulate Ad interactions with alpha vbeta 5. Incubation of Ad particles with soluble alpha vbeta 5 integrin also inhibited subsequent Ad internalization into epithelial cells as well as virus attachment to monocytic cells. These findings suggest that soluble alpha v integrins or antagonists of these coreceptors could be used to limit infection by multiple Ad types. The generation of soluble alpha v integrins should also permit further detailed kinetic and structural analysis of Ad interactions with its coreceptors.

* Corresponding author. Mailing address: Department of Immunology, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037. Phone: (619) 784-8472. Fax: (619) 784-8472. E-mail: gnemerow{at}scripps.edu.

Journal of Virology, November 1998, p. 8669-8675, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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