This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bruni, R.
Right arrow Articles by Roizman, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bruni, R.
Right arrow Articles by Roizman, B.

 Previous Article  |  Next Article 

Journal of Virology, November 1998, p. 8525-8531, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Herpes Simplex Virus 1 Regulatory Protein ICP22 Interacts with a New Cell Cycle-Regulated Factor and Accumulates in a Cell Cycle-Dependent Fashion in Infected Cells

Renato Bruni and Bernard Roizman*

The Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, Chicago, Illinois 60637

Received 25 May 1998/Accepted 7 July 1998

The herpes simplex virus 1 infected cell protein 22 (ICP22), the product of the alpha 22 gene, is a nucleotidylylated and phosphorylated nuclear protein with properties of a transcriptional factor required for the expression of a subset of viral genes. Here, we report the following. (i) ICP22 interacts with a previously unknown cellular factor designated p78 in the yeast two-hybrid system. The p78 cDNA encodes a polypeptide with a distribution of leucines reminiscent of a leucine zipper. (ii) In uninfected and infected cells, antibody to p78 reacts with two major bands with an apparent Mr of 78,000 and two minor bands with apparent Mrs of 62,000 and 55,000. (ii) p78 also interacts with ICP22 in vitro. (iii) In uninfected cells, p78 was dispersed largely in the nucleoplasm in HeLa cells and in the nucleoplasm and cytoplasm in HEp-2 cells. After infection, p78 formed large dense bodies which did not colocalize with the viral regulatory protein ICP0. (iv) Accumulation of p78 was cell cycle dependent, being highest very early in S phase. (v) The accumulation of ICP22 in synchronized cells was highest in early S phase, in contrast to the accumulation of another protein, ICP27, which was relatively independent of the cell cycle. (vi) In the course of the cell cycle, ICP22 was transiently modified in an aberrant fashion, and this modification coincided with expression of p78. The results suggest that ICP22 interacts with and may be stabilized by cell cycle-dependent proteins.

* Corresponding author. Mailing address: The Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, 910 E. 58th St., Chicago, IL 60637. Phone: (773) 702-1898. Fax: (773) 702-1631. E-mail: bernard{at}cummings.uchicago.edu.

Journal of Virology, November 1998, p. 8525-8531, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Fraser, K. A., Rice, S. A. (2007). Herpes Simplex Virus Immediate-Early Protein ICP22 Triggers Loss of Serine 2-Phosphorylated RNA Polymerase II. J. Virol. 81: 5091-5101 [Abstract] [Full Text]  
  • Orlando, J. S., Balliet, J. W., Kushnir, A. S., Astor, T. L., Kosz-Vnenchak, M., Rice, S. A., Knipe, D. M., Schaffer, P. A. (2006). ICP22 Is Required for Wild-Type Composition and Infectivity of Herpes Simplex Virus Type 1 Virions. J. Virol. 80: 9381-9390 [Abstract] [Full Text]  
  • Cun, W., Hong, M., Liu, L.-D., Dong, C.-H., Luo, J., Li, Q.-H. (2006). Structural and functional characterization of herpes simplex virus 1 immediate-early protein infected-cell protein 22.. J Biochem 140: 67-73 [Abstract] [Full Text]  
  • Orlando, J. S., Astor, T. L., Rundle, S. A., Schaffer, P. A. (2006). The Products of the Herpes Simplex Virus Type 1 Immediate-Early US1/US1.5 Genes Downregulate Levels of S-Phase-Specific Cyclins and Facilitate Virus Replication in S-Phase Vero Cells.. J. Virol. 80: 4005-4016 [Abstract] [Full Text]  
  • Shimono, K., Shimono, Y., Shimokata, K., Ishiguro, N., Takahashi, M. (2005). Microspherule Protein 1, Mi-2{beta}, and RET Finger Protein Associate in the Nucleolus and Up-regulate Ribosomal Gene Transcription. J. Biol. Chem. 280: 39436-39447 [Abstract] [Full Text]  
  • Okumura, K., Zhao, M., DePinho, R. A., Furnari, F. B., Cavenee, W. K. (2005). From the Cover: Cellular transformation by the MSP58 oncogene is inhibited by its physical interaction with the PTEN tumor suppressor. Proc. Natl. Acad. Sci. USA 102: 2703-2706 [Abstract] [Full Text]  
  • Sun, A., Devi-Rao, G. V., Rice, M. K., Gary, L. W., Bloom, D. C., Sandri-Goldin, R. M., Ghazal, P., Wagner, E. K. (2004). Immediate-Early Expression of the Herpes Simplex Virus Type 1 ICP27 Transcript Is Not Critical for Efficient Replication In Vitro or In Vivo. J. Virol. 78: 10470-10478 [Abstract] [Full Text]  
  • Brandt, C. R., Kolb, A. W. (2003). Tyrosine 116 of the Herpes Simplex Virus Type 1 IE{alpha}22 Protein Is an Ocular Virulence Determinant and Potential Phosphorylation Site. IOVS 44: 4601-4607 [Abstract] [Full Text]  
  • Brandt, C. R., Kolb, A. W., Shah, D. D., Pumfery, A. M., Kintner, R. L., Jaehnig, E., Van Gompel, J. J. (2003). Multiple Determinants Contribute to the Virulence of HSV Ocular and CNS Infection and Identification of Serine 34 of the US1 Gene as an Ocular Disease Determinant. IOVS 44: 2657-2668 [Abstract] [Full Text]  
  • Moxley, M. J., Block, T. M., Liu, H.-C., Fraser, N. W., Perng, G.-C., Wechsler, S. L., Su, Y.-H. (2002). Herpes simplex virus type 1 infection prevents detachment of nerve growth factor-differentiated PC12 cells in culture. J. Gen. Virol. 83: 1591-1600 [Abstract] [Full Text]  
  • Winkler, M. T., Schang, L. S., Doster, A., Holt, T., Jones, C. (2000). Analysis of cyclins in trigeminal ganglia of calves infected with bovine herpesvirus-1. J. Gen. Virol. 81: 2993-2998 [Abstract] [Full Text]  
  • Derbigny, W. A., Kim, S. K., Caughman, G. B., O'Callaghan, D. J. (2000). The EICP22 Protein of Equine Herpesvirus 1 Physically Interacts with the Immediate-Early Protein and with Itself To Form Dimers and Higher-Order Complexes. J. Virol. 74: 1425-1435 [Abstract] [Full Text]  
  • Advani, S. J., Brandimarti, R., Weichselbaum, R. R., Roizman, B. (2000). The Disappearance of Cyclins A and B and the Increase in Activity of the G2/M-Phase Cellular Kinase cdc2 in Herpes Simplex Virus 1-Infected Cells Require Expression of the alpha 22/US1.5 and UL13 Viral Genes. J. Virol. 74: 8-15 [Abstract] [Full Text]  
  • Markovitz, N. S., Filatov, F., Roizman, B. (1999). The UL3 Protein of Herpes Simplex Virus 1 Is Translated Predominantly from the Second In-Frame Methionine Codon and Is Subject to at Least Two Posttranslational Modifications. J. Virol. 73: 8010-8018 [Abstract] [Full Text]  
  • Hobbs, W. E. II, DeLuca, N. A. (1999). Perturbation of Cell Cycle Progression and Cellular Gene Expression as a Function of Herpes Simplex Virus ICP0. J. Virol. 73: 8245-8255 [Abstract] [Full Text]  
  • Bruni, R., Fineschi, B., Ogle, W. O., Roizman, B. (1999). A Novel Cellular Protein, p60, Interacting with both Herpes Simplex Virus 1 Regulatory Proteins ICP22 and ICP0 Is Modified in a Cell-Type-Specific Manner and Is Recruited to the Nucleus after Infection. J. Virol. 73: 3810-3817 [Abstract] [Full Text]  
  • Ogle, W. O., Roizman, B. (1999). Functional Anatomy of Herpes Simplex Virus 1 Overlapping Genes Encoding Infected-Cell Protein 22 and US1.5 Protein. J. Virol. 73: 4305-4315 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»