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Journal of Virology, November 1998, p. 8472-8476, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Canine Distemper Virus DNA Vaccination Induces Humoral and Cellular Immunity and Protects against a Lethal Intracerebral Challenge

Nathalie Sixt, Alicia Cardoso, Agnès Vallier, Joël Fayolle, Robin Buckland, and T. Fabian Wild*

Unité INSERM 404, Immunity and Vaccination, Bâtiment Ex-Institut Pasteur de Lyon, 69365 Lyon Cedex 07, France

Received 11 May 1998/Accepted 6 August 1998

We have studied the immune responses to the two glycoproteins of the Morbillivirus canine distemper virus (CDV) after DNA vaccination of BALB/c mice. The plasmids coding for both CDV hemagglutinin (H) and fusion protein (F) induce high levels of antibodies which persist for more than 6 months. Intramuscular inoculation of the CDV DNA induces a predominantly immunoglobulin G2a (IgG2a) response (Th1 response), whereas gene gun immunization with CDV H evokes exclusively an IgG1 response (Th2 response). In contrast, the CDV F gene elicited a mixed, IgG1 and IgG2a response. Mice vaccinated (by gene gun) with either the CDV H or F DNA showed a class I-restricted cytotoxic lymphocyte response. Immunized mice challenged intracerebrally with a lethal dose of a neurovirulent strain of CDV were protected. However, approximately 30% of the mice vaccinated with the CDV F DNA became obese in the first 2 months following the challenge. This was not correlated with the serum antibody levels.


* Corresponding author. Mailing address: Unité INSERM 404, Immunity and Vaccination, Bâtiment Ex-Institut Pasteur de Lyon, Avenue Tony Garnier, 69365 Lyon Cedex 07, France. Phone: 33 4 72 72 25 53. Fax: 33 4 72 72 25 67. E-mail: wild{at}lyon151.inserm.fr.


Journal of Virology, November 1998, p. 8472-8476, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.



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Copyright © 1998 by the American Society for Microbiology. All rights reserved.