This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dull, T.
Right arrow Articles by Naldini, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dull, T.
Right arrow Articles by Naldini, L.

Next Article 

Journal of Virology, November 1998, p. 8463-8471, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Third-Generation Lentivirus Vector with a Conditional Packaging System

Tom Dull,1 Romain Zufferey,2 Michael Kelly,1 R. J. Mandel,1 Minh Nguyen,1 Didier Trono,2 and Luigi Naldini1,*

Cell Genesys, Foster City, California,1 and Department of Genetics and Microbiology, University of Geneva Medical School, Geneva, Switzerland2

Received 1 June 1998/Accepted 21 July 1998

Vectors derived from human immunodeficiency virus (HIV) are highly efficient vehicles for in vivo gene delivery. However, their biosafety is of major concern. Here we exploit the complexity of the HIV genome to provide lentivirus vectors with novel biosafety features. In addition to the structural genes, HIV contains two regulatory genes, tat and rev, that are essential for HIV replication, and four accessory genes that encode critical virulence factors. We previously reported that the HIV type 1 accessory open reading frames are dispensable for efficient gene transduction by a lentivirus vector. We now demonstrate that the requirement for the tat gene can be offset by placing constitutive promoters upstream of the vector transcript. Vectors generated from constructs containing such a chimeric long terminal repeat (LTR) transduced neurons in vivo at very high efficiency, whether or not they were produced in the presence of Tat. When the rev gene was also deleted from the packaging construct, expression of gag and pol was strictly dependent on Rev complementation in trans. By the combined use of a separate nonoverlapping Rev expression plasmid and a 5' LTR chimeric transfer construct, we achieved optimal yields of vector of high transducing efficiency (up to 107 transducing units [TU]/ml and 104 TU/ng of p24). This third-generation lentivirus vector uses only a fractional set of HIV genes: gag, pol, and rev. Moreover, the HIV-derived constructs, and any recombinant between them, are contingent on upstream elements and trans complementation for expression and thus are nonfunctional outside of the vector producer cells. This split-genome, conditional packaging system is based on existing viral sequences and acts as a built-in device against the generation of productive recombinants. While the actual biosafety of the vector will ultimately be proven in vivo, the improved design presented here should facilitate testing of lentivirus vectors.

* Corresponding author. Mailing address: Cell Genesys, 342 Lakeside Dr., Foster City, CA 94404. Phone: (650) 425-4474. Fax: (650) 358-8636. E-mail: luigin{at}cellgenesys.com.

Journal of Virology, November 1998, p. 8463-8471, Vol. 72, No. 11
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Delous, M., Hellman, N. E., Gaude, H.-M., Silbermann, F., Le Bivic, A., Salomon, R., Antignac, C., Saunier, S. (2009). Nephrocystin-1 and nephrocystin-4 are required for epithelial morphogenesis and associate with PALS1/PATJ and Par6. Hum Mol Genet 18: 4711-4723 [Abstract] [Full Text]  
  • Brohm, C., Steinmann, E., Friesland, M., Lorenz, I. C., Patel, A., Penin, F., Bartenschlager, R., Pietschmann, T. (2009). Characterization of Determinants Important for Hepatitis C Virus p7 Function in Morphogenesis by Using trans-Complementation. J. Virol. 83: 11682-11693 [Abstract] [Full Text]  
  • Lee, H.-M., Yuk, J.-M., Shin, D.-M., Yang, C.-S., Kim, K.-K., Choi, D.-K., Liang, Z.-L., Kim, J.-M., Jeon, B. H., Kim, C. D., Lee, J.-H., Jo, E.-K. (2009). Apurinic/Apyrimidinic Endonuclease 1 Is a Key Modulator of Keratinocyte Inflammatory Responses. J. Immunol. 183: 6839-6848 [Abstract] [Full Text]  
  • Ganjam, G. K., Dimova, E. Y., Unterman, T. G., Kietzmann, T. (2009). FoxO1 and HNF-4 Are Involved in Regulation of Hepatic Glucokinase Gene Expression by Resveratrol. J. Biol. Chem. 284: 30783-30797 [Abstract] [Full Text]  
  • Trotard, M., Lepere-Douard, C., Regeard, M., Piquet-Pellorce, C., Lavillette, D., Cosset, F.-L., Gripon, P., Le Seyec, J. (2009). Kinases required in hepatitis C virus entry and replication highlighted by small interference RNA screening. FASEB J. 23: 3780-3789 [Abstract] [Full Text]  
  • Attwooll, C. L., Akpinar, M., Petrini, J. H. J. (2009). The Mre11 Complex and the Response to Dysfunctional Telomeres. Mol. Cell. Biol. 29: 5540-5551 [Abstract] [Full Text]  
  • Smith, K. D., Fu, M. A., Brown, E. J. (2009). Tim-Tipin dysfunction creates an indispensible reliance on the ATR-Chk1 pathway for continued DNA synthesis. JCB 187: 15-23 [Abstract] [Full Text]  
  • Unger, C., Gao, S., Cohen, M., Jaconi, M., Bergstrom, R., Holm, F., Galan, A., Sanchez, E., Irion, O., Dubuisson, J. B., Giry-Laterriere, M., Salmon, P., Simon, C., Hovatta, O., Feki, A. (2009). Immortalized human skin fibroblast feeder cells support growth and maintenance of both human embryonic and induced pluripotent stem cells. Hum Reprod 24: 2567-2581 [Abstract] [Full Text]  
  • Sugiyama, R., Habu, Y., Ohnari, A., Miyano-Kurosaki, N., Takaku, H. (2009). RNA Interference Targeted to the Conserved Dimerization Initiation Site (DIS) of HIV-1 Restricts Virus Escape Mutation. J Biochem 146: 481-489 [Abstract] [Full Text]  
  • Zinke, M., Kendl, S., Singethan, K., Fehrholz, M., Reuter, D., Rennick, L., Herold, M. J., Schneider-Schaulies, J. (2009). Clearance of Measles Virus from Persistently Infected Cells by Short Hairpin RNA. J. Virol. 83: 9423-9431 [Abstract] [Full Text]  
  • Karanikolas, B. D.W., Figueiredo, M. L., Wu, L. (2009). Polycomb Group Protein Enhancer of Zeste 2 Is an Oncogene That Promotes the Neoplastic Transformation of a Benign Prostatic Epithelial Cell Line. Mol Cancer Res 7: 1456-1465 [Abstract] [Full Text]  
  • Rossello, R. A., Wang, Z., Kizana, E., Krebsbach, P. H., Kohn, D. H. (2009). From the Cover: Connexin 43 as a signaling platform for increasing the volume and spatial distribution of regenerated tissue. Proc. Natl. Acad. Sci. USA 106: 13219-13224 [Abstract] [Full Text]  
  • Lu, J., Verma, S. C., Murakami, M., Cai, Q., Kumar, P., Xiao, B., Robertson, E. S. (2009). Latency-Associated Nuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus (KSHV) Upregulates Survivin Expression in KSHV-Associated B-Lymphoma Cells and Contributes to Their Proliferation. J. Virol. 83: 7129-7141 [Abstract] [Full Text]  
  • Hosking, B., Francois, M., Wilhelm, D., Orsenigo, F., Caprini, A., Svingen, T., Tutt, D., Davidson, T., Browne, C., Dejana, E., Koopman, P. (2009). Sox7 and Sox17 are strain-specific modifiers of the lymphangiogenic defects caused by Sox18 dysfunction in mice. Development 136: 2385-2391 [Abstract] [Full Text]  
  • Colombetti, S., Levy, F., Chapatte, L. (2009). IL-7 adjuvant treatment enhances long-term tumor antigen-specific CD8+ T-cell responses after immunization with recombinant lentivector. Blood 113: 6629-6637 [Abstract] [Full Text]  
  • Holopainen, T., Huang, H., Chen, C., Kim, K. E., Zhang, L., Zhou, F., Han, W., Li, C., Yu, J., Wu, J., Koh, G. Y., Alitalo, K., He, Y. (2009). Angiopoietin-1 Overexpression Modulates Vascular Endothelium to Facilitate Tumor Cell Dissemination and Metastasis Establishment. Cancer Res. 69: 4656-4664 [Abstract] [Full Text]  
  • Green, L. A., Liu, Y., He, J. J. (2009). Inhibition of HIV-1 Infection and Replication by Enhancing Viral Incorporation of Innate Anti-HIV-1 Protein A3G: A NON-PATHOGENIC Nef MUTANT-BASED ANTI-HIV STRATEGY. J. Biol. Chem. 284: 13363-13372 [Abstract] [Full Text]  
  • van den Biggelaar, M., Bouwens, E. A.M., Kootstra, N. A., Hebbel, R. P., Voorberg, J., Mertens, K. (2009). Storage and regulated secretion of factor VIII in blood outgrowth endothelial cells. haematol 94: 670-678 [Abstract] [Full Text]  
  • van Balkom, B. W.M., Boone, M., Hendriks, G., Kamsteeg, E.-J., Robben, J. H., Stronks, H. C., van der Voorde, A., van Herp, F., van der Sluijs, P., Deen, P. M.T. (2009). LIP5 Interacts with Aquaporin 2 and Facilitates Its Lysosomal Degradation. J. Am. Soc. Nephrol. 20: 990-1001 [Abstract] [Full Text]  
  • Sadagopan, S., Sharma-Walia, N., Veettil, M. V., Bottero, V., Levine, R., Vart, R. J., Chandran, B. (2009). Kaposi's Sarcoma-Associated Herpesvirus Upregulates Angiogenin during Infection of Human Dermal Microvascular Endothelial Cells, Which Induces 45S rRNA Synthesis, Antiapoptosis, Cell Proliferation, Migration, and Angiogenesis. J. Virol. 83: 3342-3364 [Abstract] [Full Text]  
  • Urtishak, K. A., Smith, K. D., Chanoux, R. A., Greenberg, R. A., Johnson, F. B., Brown, E. J. (2009). Timeless Maintains Genomic Stability and Suppresses Sister Chromatid Exchange during Unperturbed DNA Replication. J. Biol. Chem. 284: 8777-8785 [Abstract] [Full Text]  
  • Her, Y. R., Chung, I. K. (2009). Ubiquitin Ligase RLIM Modulates Telomere Length Homeostasis through a Proteolysis of TRF1. J. Biol. Chem. 284: 8557-8566 [Abstract] [Full Text]  
  • Li, B., VanRoey, M., Wang, C., Chen, T.-h. T., Korman, A., Jooss, K. (2009). Anti-Programmed Death-1 Synergizes with Granulocyte Macrophage Colony-Stimulating Factor-Secreting Tumor Cell Immunotherapy Providing Therapeutic Benefit to Mice with Established Tumors. Clin. Cancer Res. 15: 1623-1634 [Abstract] [Full Text]  
  • Chanoux, R. A., Yin, B., Urtishak, K. A., Asare, A., Bassing, C. H., Brown, E. J. (2009). ATR and H2AX Cooperate in Maintaining Genome Stability under Replication Stress. J. Biol. Chem. 284: 5994-6003 [Abstract] [Full Text]  
  • Zhou, S., Kurt-Jones, E. A., Cerny, A. M., Chan, M., Bronson, R. T., Finberg, R. W. (2009). MyD88 Intrinsically Regulates CD4 T-Cell Responses. J. Virol. 83: 1625-1634 [Abstract] [Full Text]  
  • Knuesel, M. T., Meyer, K. D., Donner, A. J., Espinosa, J. M., Taatjes, D. J. (2009). The Human CDK8 Subcomplex Is a Histone Kinase That Requires Med12 for Activity and Can Function Independently of Mediator. Mol. Cell. Biol. 29: 650-661 [Abstract] [Full Text]  
  • Baril, M., Racine, M.-E., Penin, F., Lamarre, D. (2009). MAVS Dimer Is a Crucial Signaling Component of Innate Immunity and the Target of Hepatitis C Virus NS3/4A Protease. J. Virol. 83: 1299-1311 [Abstract] [Full Text]  
  • Chien, A. J., Moore, E. C., Lonsdorf, A. S., Kulikauskas, R. M., Rothberg, B. G., Berger, A. J., Major, M. B., Hwang, S. T., Rimm, D. L., Moon, R. T. (2009). Activated Wnt/ss-catenin signaling in melanoma is associated with decreased proliferation in patient tumors and a murine melanoma model. Proc. Natl. Acad. Sci. USA 106: 1193-1198 [Abstract] [Full Text]  
  • Adelman, C. A., De, S., Petrini, J. H. J. (2009). Rad50 Is Dispensable for the Maintenance and Viability of Postmitotic Tissues. Mol. Cell. Biol. 29: 483-492 [Abstract] [Full Text]  
  • Bulat, N., Waeber, G., Widmann, C. (2009). LDLs stimulate p38 MAPKs and wound healing through SR-BI independently of Ras and PI3 kinase. J. Lipid Res. 50: 81-89 [Abstract] [Full Text]  
  • Pearson, R., Kim, Y. K., Hokello, J., Lassen, K., Friedman, J., Tyagi, M., Karn, J. (2008). Epigenetic Silencing of Human Immunodeficiency Virus (HIV) Transcription by Formation of Restrictive Chromatin Structures at the Viral Long Terminal Repeat Drives the Progressive Entry of HIV into Latency. J. Virol. 82: 12291-12303 [Abstract] [Full Text]  
  • Rio, P., Meza, N. W., Gonzalez-Murillo, A., Navarro, S., Alvarez, L., Surralles, J., Castella, M., Guenechea, G., Segovia, J. C., Hanenberg, H., Bueren, J. A. (2008). In vivo proliferation advantage of genetically corrected hematopoietic stem cells in a mouse model of Fanconi anemia FA-D1. Blood 112: 4853-4861 [Abstract] [Full Text]  
  • Richardson, M. W., Carroll, R. G., Stremlau, M., Korokhov, N., Humeau, L. M., Silvestri, G., Sodroski, J., Riley, J. L. (2008). Mode of Transmission Affects the Sensitivity of Human Immunodeficiency Virus Type 1 to Restriction by Rhesus TRIM5{alpha}. J. Virol. 82: 11117-11128 [Abstract] [Full Text]  
  • Liebner, S., Corada, M., Bangsow, T., Babbage, J., Taddei, A., Czupalla, C. J., Reis, M., Felici, A., Wolburg, H., Fruttiger, M., Taketo, M. M., von Melchner, H., Plate, K. H., Gerhardt, H., Dejana, E. (2008). Wnt/{beta}-catenin signaling controls development of the blood-brain barrier. JCB 183: 409-417 [Abstract] [Full Text]  
  • Zhou, L. L., Zhao, Y., Ringrose, A., DeGeer, D., Kennah, E., Lin, A. E.-J., Sheng, G., Li, X.-J., Turhan, A., Jiang, X. (2008). AHI-1 interacts with BCR-ABL and modulates BCR-ABL transforming activity and imatinib response of CML stem/progenitor cells. JEM 205: 2657-2671 [Abstract] [Full Text]  
  • Kim, S.-h., Davalos, A. R., Heo, S.-J., Rodier, F., Zou, Y., Beausejour, C., Kaminker, P., Yannone, S. M., Campisi, J. (2008). Telomere dysfunction and cell survival: roles for distinct TIN2-containing complexes. JCB 181: 447-460 [Abstract] [Full Text]  
  • Jin, Q., Marsh, J., Cornetta, K., Alkhatib, G. (2008). Resistance to human immunodeficiency virus type 1 (HIV-1) generated by lentivirus vector-mediated delivery of the CCR5{Delta}32 gene despite detectable expression of the HIV-1 co-receptors. J. Gen. Virol. 89: 2611-2621 [Abstract] [Full Text]  
  • Park, F., Sweeney, W. E., Jia, G., Roman, R. J., Avner, E. D. (2008). 20-HETE Mediates Proliferation of Renal Epithelial Cells in Polycystic Kidney Disease. J. Am. Soc. Nephrol. 19: 1929-1939 [Abstract] [Full Text]  
  • Cantaluppi, V., Biancone, L., Romanazzi, G. M., Figliolini, F., Beltramo, S., Galimi, F., Camboni, M. G., Deriu, E., Conaldi, P., Bottelli, A., Orlandi, V., Herrera, M. B., Pacitti, A., Segoloni, G. P., Camussi, G. (2008). Macrophage Stimulating Protein May Promote Tubular Regeneration after Acute Injury. J. Am. Soc. Nephrol. 19: 1904-1918 [Abstract] [Full Text]  
  • McCully, R. R., Pomerantz, J. L. (2008). The Protein Kinase C-Responsive Inhibitory Domain of CARD11 Functions in NF-{kappa}B Activation To Regulate the Association of Multiple Signaling Cofactors That Differentially Depend on Bcl10 and MALT1 for Association. Mol. Cell. Biol. 28: 5668-5686 [Abstract] [Full Text]  
  • Vogiatzi, T., Xilouri, M., Vekrellis, K., Stefanis, L. (2008). Wild Type {alpha}-Synuclein Is Degraded by Chaperone-mediated Autophagy and Macroautophagy in Neuronal Cells. J. Biol. Chem. 283: 23542-23556 [Abstract] [Full Text]  
  • Desy, O., Carignan, D., Caruso, M., de Campos-Lima, P. O. (2008). Immunosuppressive Effect of Isopropanol: Down-Regulation of Cytokine Production Results from the Alteration of Discrete Transcriptional Pathways in Activated Lymphocytes. J. Immunol. 181: 2348-2355 [Abstract] [Full Text]  
  • Orso, F., Penna, E., Cimino, D., Astanina, E., Maione, F., Valdembri, D., Giraudo, E., Serini, G., Sismondi, P., De Bortoli, M., Taverna, D. (2008). AP-2{alpha} and AP-2{gamma} regulate tumor progression via specific genetic programs. FASEB J. 22: 2702-2714 [Abstract] [Full Text]  
  • Niapour, M., Yu, Y., Berger, S. A. (2008). Regulation of Calpain Activity by c-Myc through Calpastatin and Promotion of Transformation in c-Myc-negative Cells by Calpastatin Suppression. J. Biol. Chem. 283: 21371-21381 [Abstract] [Full Text]  
  • Steinmann, E., Brohm, C., Kallis, S., Bartenschlager, R., Pietschmann, T. (2008). Efficient trans-Encapsidation of Hepatitis C Virus RNAs into Infectious Virus-Like Particles. J. Virol. 82: 7034-7046 [Abstract] [Full Text]  
  • Miklossy, G., Tozser, J., Kadas, J., Ishima, R., Louis, J. M., Bagossi, P. (2008). Novel macromolecular inhibitors of human immunodeficiency virus-1 protease. Protein Eng Des Sel 21: 453-461 [Abstract] [Full Text]  
  • Trobridge, G. D., Beard, B. C., Gooch, C., Wohlfahrt, M., Olsen, P., Fletcher, J., Malik, P., Kiem, H.-P. (2008). Efficient transduction of pigtailed macaque hematopoietic repopulating cells with HIV-based lentiviral vectors. Blood 111: 5537-5543 [Abstract] [Full Text]  
  • Sebestyen, Z., Schooten, E., Sals, T., Zaldivar, I., San Jose, E., Alarcon, B., Bobisse, S., Rosato, A., Szollosi, J., Gratama, J. W., Willemsen, R. A., Debets, R. (2008). Human TCR That Incorporate CD3{zeta} Induce Highly Preferred Pairing between TCR{alpha} and {beta} Chains following Gene Transfer. J. Immunol. 180: 7736-7746 [Abstract] [Full Text]  
  • Li, B., VanRoey, M., Triebel, F., Jooss, K. (2008). Lymphocyte Activation Gene-3 Fusion Protein Increases the Potency of a Granulocyte Macrophage Colony-Stimulating Factor-Secreting Tumor Cell Immunotherapy. Clin. Cancer Res. 14: 3545-3554 [Abstract] [Full Text]  
  • El-Amouri, S. S., Zhu, H., Yu, J., Marr, R., Verma, I. M., Kindy, M. S. (2008). Neprilysin: An Enzyme Candidate to Slow the Progression of Alzheimer's Disease. Am. J. Pathol. 172: 1342-1354 [Abstract] [Full Text]  
  • Schuler, A., Schwieger, M., Engelmann, A., Weber, K., Horn, S., Muller, U., Arnold, M. A., Olson, E. N., Stocking, C. (2008). The MADS transcription factor Mef2c is a pivotal modulator of myeloid cell fate. Blood 111: 4532-4541 [Abstract] [Full Text]  
  • Kim, C., Wilcox-Adelman, S., Sano, Y., Tang, W.-J., Collier, R. J., Park, J. M. (2008). From the Cover: Antiinflammatory cAMP signaling and cell migration genes co-opted by the anthrax bacillus. Proc. Natl. Acad. Sci. USA 105: 6150-6155 [Abstract] [Full Text]  
  • Abdel-Azim, H., Zhu, Y., Hollis, R., Wang, X., Ge, S., Hao, Q.-L., Smbatyan, G., Kohn, D. B., Rosol, M., Crooks, G. M. (2008). Expansion of multipotent and lymphoid-committed human progenitors through intracellular dimerization of Mpl. Blood 111: 4064-4074 [Abstract] [Full Text]  
  • Lasiene, J., Shupe, L., Perlmutter, S., Horner, P. (2008). No Evidence for Chronic Demyelination in Spared Axons after Spinal Cord Injury in a Mouse. J. Neurosci. 28: 3887-3896 [Abstract] [Full Text]  
  • Terrovitis, J., Stuber, M., Youssef, A., Preece, S., Leppo, M., Kizana, E., Schar, M., Gerstenblith, G., Weiss, R. G., Marban, E., Abraham, M. R. (2008). Magnetic Resonance Imaging Overestimates Ferumoxide-Labeled Stem Cell Survival After Transplantation in the Heart. Circulation 117: 1555-1562 [Abstract] [Full Text]  
  • Yu, Y., Niapour, M., Zhang, Y., Berger, S. A. (2008). Mitochondrial regulation by c-Myc and hypoxia-inducible factor-1{alpha} controls sensitivity to econazole. Molecular Cancer Therapeutics 7: 483-491 [Abstract] [Full Text]  
  • Seiden-Long, I., Navab, R., Shih, W., Li, M., Chow, J., Zhu, C. Q., Radulovich, N., Saucier, C., Tsao, M.-S. (2008). Gab1 but not Grb2 mediates tumor progression in Met overexpressing colorectal cancer cells. Carcinogenesis 29: 647-655 [Abstract] [Full Text]  
  • Nilakantan, V., Maenpaa, C., Jia, G., Roman, R. J., Park, F. (2008). 20-HETE-mediated cytotoxicity and apoptosis in ischemic kidney epithelial cells. Am. J. Physiol. Renal Physiol. 294: F562-F570 [Abstract] [Full Text]  
  • Liu, Y., Lagowski, J., Sundholm, A., Sundberg, A., Kulesz-Martin, M. (2007). Microtubule Disruption and Tumor Suppression by Mitogen-Activated Protein Kinase Phosphatase 4. Cancer Res. 67: 10711-10719 [Abstract] [Full Text]  
  • Uo, T., Kinoshita, Y., Morrison, R. S. (2007). Apoptotic Actions of p53 Require Transcriptional Activation of PUMA and Do Not Involve a Direct Mitochondrial/Cytoplasmic Site of Action in Postnatal Cortical Neurons. J. Neurosci. 27: 12198-12210 [Abstract] [Full Text]  
  • Sekar, R. B., Kizana, E., Smith, R. R., Barth, A. S., Zhang, Y., Marban, E., Tung, L. (2007). Lentiviral vector-mediated expression of GFP or Kir2.1 alters the electrophysiology of neonatal rat ventricular myocytes without inducing cytotoxicity. Am. J. Physiol. Heart Circ. Physiol. 293: H2757-H2770 [Abstract] [Full Text]  
  • Vladar, E. K., Stearns, T. (2007). Molecular characterization of centriole assembly in ciliated epithelial cells. JCB 178: 31-42 [Abstract] [Full Text]  
  • Brugmann, S. A., Goodnough, L. H., Gregorieff, A., Leucht, P., ten Berge, D., Fuerer, C., Clevers, H., Nusse, R., Helms, J. A. (2007). Wnt signaling mediates regional specification in the vertebrate face. Development 134: 3283-3295 [Abstract] [Full Text]  
  • Cattoglio, C., Facchini, G., Sartori, D., Antonelli, A., Miccio, A., Cassani, B., Schmidt, M., von Kalle, C., Howe, S., Thrasher, A. J., Aiuti, A., Ferrari, G., Recchia, A., Mavilio, F. (2007). Hot spots of retroviral integration in human CD34+ hematopoietic cells. Blood 110: 1770-1778 [Abstract] [Full Text]  
  • Gierman, H. J., Indemans, M. H.G., Koster, J., Goetze, S., Seppen, J., Geerts, D., van Driel, R., Versteeg, R. (2007). Domain-wide regulation of gene expression in the human genome. Genome Res 17: 1286-1295 [Abstract] [Full Text]  
  • Barbaro, V., Testa, A., Di Iorio, E., Mavilio, F., Pellegrini, G., De Luca, M. (2007). C/EBP{delta} regulates cell cycle and self-renewal of human limbal stem cells. JCB 177: 1037-1049 [Abstract] [Full Text]  
  • Liao, W., Nguyen, M. T. A., Yoshizaki, T., Favelyukis, S., Patsouris, D., Imamura, T., Verma, I. M., Olefsky, J. M. (2007). Suppression of PPAR-{gamma} attenuates insulin-stimulated glucose uptake by affecting both GLUT1 and GLUT4 in 3T3-L1 adipocytes. Am. J. Physiol. Endocrinol. Metab. 293: E219-E227 [Abstract] [Full Text]  
  • Smyth, C. M, Ginn, S. L, Deakin, C. T, Logan, G. J, Alexander, I. E (2007). Limiting {gamma}c expression differentially affects signaling via the interleukin (IL)-7 and IL-15 receptors. Blood 110: 91-98 [Abstract] [Full Text]  
  • Sanyal, M., Tung, J. W., Karsunky, H., Zeng, H., Selleri, L., Weissman, I. L., Herzenberg, L. A., Cleary, M. L. (2007). B-cell development fails in the absence of the Pbx1 proto-oncogene. Blood 109: 4191-4199 [Abstract] [Full Text]  
  • Schaller, T., Appel, N., Koutsoudakis, G., Kallis, S., Lohmann, V., Pietschmann, T., Bartenschlager, R. (2007). Analysis of Hepatitis C Virus Superinfection Exclusion by Using Novel Fluorochrome Gene-Tagged Viral Genomes. J. Virol. 81: 4591-4603 [Abstract] [Full Text]  
  • Chang, H.-W., Chu, T.-S., Huang, H.-Y., Chueh, S.-C., Wu, V.-C., Chen, Y.-M., Hsieh, B.-S., Wu, K.-D. (2007). Down-Regulation of D2 Dopamine Receptor and Increased Protein Kinase C{micro} Phosphorylation in Aldosterone-Producing Adenoma Play Roles in Aldosterone Overproduction. J. Clin. Endocrinol. Metab. 92: 1863-1870 [Abstract] [Full Text]  
  • Li, T.-H., Zhao, H., Peng, Y., Beliakoff, J., Brooks, J. D., Sun, Z. (2007). A promoting role of androgen receptor in androgen-sensitive and -insensitive prostate cancer cells. Nucleic Acids Res 0: gkm198v1-10 [Abstract] [Full Text]  
  • Garcia-Rudaz, C., Luna, F., Tapia, V., Kerr, B., Colgin, L., Galimi, F., Dissen, G. A., Rawlings, N. D., Ojeda, S. R. (2007). Fxna, a novel gene differentially expressed in the rat ovary at the time of folliculogenesis, is required for normal ovarian histogenesis. Development 134: 945-957 [Abstract] [Full Text]  
  • Fries, B., Heukeshoven, J., Hauber, I., Gruttner, C., Stocking, C., Kehlenbach, R. H., Hauber, J., Chemnitz, J. (2007). Analysis of Nucleocytoplasmic Trafficking of the HuR Ligand APRIL and Its Influence on CD83 Expression. J. Biol. Chem. 282: 4504-4515 [Abstract] [Full Text]  
  • Balabanov, S., Gontarewicz, A., Ziegler, P., Hartmann, U., Kammer, W., Copland, M., Brassat, U., Priemer, M., Hauber, I., Wilhelm, T., Schwarz, G., Kanz, L., Bokemeyer, C., Hauber, J., Holyoake, T. L., Nordheim, A., Brummendorf, T. H. (2007). Hypusination of eukaryotic initiation factor 5A (eIF5A): a novel therapeutic target in BCR-ABL-positive leukemias identified by a proteomics approach. Blood 109: 1701-1711 [Abstract] [Full Text]  
  • Clement, S., Stouffs, M., Bettiol, E., Kampf, S., Krause, K.-H., Chaponnier, C., Jaconi, M. (2007). Expression and function of {alpha}-smooth muscle actin during embryonic-stem-cell-derived cardiomyocyte differentiation. J. Cell Sci. 120: 229-238 [Abstract] [Full Text]  
  • Akazawa, T., Ebihara, T., Okuno, M., Okuda, Y., Shingai, M., Tsujimura, K., Takahashi, T., Ikawa, M., Okabe, M., Inoue, N., Okamoto-Tanaka, M., Ishizaki, H., Miyoshi, J., Matsumoto, M., Seya, T. (2007). Antitumor NK activation induced by the Toll-like receptor 3-TICAM-1 (TRIF) pathway in myeloid dendritic cells. Proc. Natl. Acad. Sci. USA 104: 252-257 [Abstract] [Full Text]  
  • Li, Q., Wang, X., Wu, X., Rui, Y., Liu, W., Wang, J., Wang, X., Liou, Y.-C., Ye, Z., Lin, S.-C. (2007). Daxx Cooperates with the Axin/HIPK2/p53 Complex to Induce Cell Death. Cancer Res. 67: 66-74 [Abstract] [Full Text]  
  • Thyssen, G., Li, T.-H., Lehmann, L., Zhuo, M., Sharma, M., Sun, Z. (2006). LZTS2 Is a Novel {beta}-Catenin-Interacting Protein and Regulates the Nuclear Export of {beta}-Catenin. Mol. Cell. Biol. 26: 8857-8867 [Abstract] [Full Text]  
  • Colmone, A., Li, S., Wang, C.-R. (2006). Activating Transcription Factor/cAMP Response Element Binding Protein Family Member Regulated Transcription of CD1A. J. Immunol. 177: 7024-7032 [Abstract] [Full Text]  
  • Mantovani, J., Holic, N., Martinez, K., Danos, O., Perea, J. (2006). A high throughput method for genome-wide analysis of retroviral integration. Nucleic Acids Res 34: e134-e134 [Abstract] [Full Text]  
  • Langelier, C., von Schwedler, U. K., Fisher, R. D., De Domenico, I., White, P. L., Hill, C. P., Kaplan, J., Ward, D., Sundquist, W. I. (2006). Human ESCRT-II Complex and Its Role in Human Immunodeficiency Virus Type 1 Release. J. Virol. 80: 9465-9480 [Abstract] [Full Text]  
  • Borello, U., Berarducci, B., Murphy, P., Bajard, L., Buffa, V., Piccolo, S., Buckingham, M., Cossu, G. (2006). The Wnt/{beta}-catenin pathway regulates Gli-mediated Myf5 expression during somitogenesis. Development 133: 3723-3732 [Abstract] [Full Text]  
  • Shin, K.-J., Wall, E. A., Zavzavadjian, J. R., Santat, L. A., Liu, J., Hwang, J.-I., Rebres, R., Roach, T., Seaman, W., Simon, M. I., Fraser, I. D. C. (2006). A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Proc. Natl. Acad. Sci. USA 103: 13759-13764 [Abstract] [Full Text]  
  • Groot, F., Sanders, R. W., ter Brake, O., Nazmi, K., Veerman, E. C. I., Bolscher, J. G. M., Berkhout, B. (2006). Histatin 5-derived Peptide with improved fungicidal properties enhances human immunodeficiency virus type 1 replication by promoting viral entry.. J. Virol. 80: 9236-9243 [Abstract] [Full Text]  
  • Silvertown, J. D., Walia, J. S., Summerlee, A. J., Medin, J. A. (2006). Functional Expression of Mouse Relaxin and Mouse Relaxin-3 in the Lung from an Ebola Virus Glycoprotein-Pseudotyped Lentivirus via Tracheal Delivery. Endocrinology 147: 3797-3808 [Abstract] [Full Text]  
  • Tatano, Y., Takahashi, T., Tsuji, D., Takeuchi, N., Tsuta, K., Takada, G., Ohsawa, M., Sakuraba, H., Itoh, K. (2006). Significant Decrease in Tropoelastin Gene Expression in Fibroblasts from a Japanese Costello Syndrome Patient with Impaired Elastogenesis and Enhanced Proliferation. J Biochem 140: 193-200 [Abstract] [Full Text]  
  • Clemenceau, B., Congy-Jolivet, N., Gallot, G., Vivien, R., Gaschet, J., Thibault, G., Vie, H. (2006). Antibody-dependent cellular cytotoxicity (ADCC) is mediated by genetically modified antigen-specific human T lymphocytes. Blood 107: 4669-4677 [Abstract] [Full Text]  
  • Koutsoudakis, G., Kaul, A., Steinmann, E., Kallis, S., Lohmann, V., Pietschmann, T., Bartenschlager, R. (2006). Characterization of the early steps of hepatitis C virus infection by using luciferase reporter viruses.. J. Virol. 80: 5308-5320 [Abstract] [Full Text]  
  • Kim, B., Koo, H., Yang, S., Bang, S., Jung, Y., Kim, Y., Kim, J., Park, J., Moon, R. T., Song, K., Lee, I. (2006). TC1(C8orf4) Correlates with Wnt/{beta}-Catenin Target Genes and Aggressive Biological Behavior in Gastric Cancer.. Clin. Cancer Res. 12: 3541-3548 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»