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Journal of Virology, October 1998, p. 8430-8436, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Optimal Induction of Hepatitis C Virus Envelope-Specific Immunity by Bicistronic Plasmid DNA Inoculation with the Granulocyte-Macrophage Colony-Stimulating Factor Gene

Seung Woo Lee, Jae Ho Cho, and Young Chul Sung*

Department of Life Science, Center for Biofunctional Molecules, School of Environmental Engineering, Pohang University of Science and Technology, Hyoja Dong, Pohang, 790-784 Korea

Received 30 April 1998/Accepted 1 July 1998

In this study, we have constructed various DNA vaccine vectors that carried hepatitis C virus (HCV) envelope genes without and with the granulocyte-macrophage colony-stimulating factor (GM-CSF) gene in several different ways. In Buffalo rats that received plasmids carrying the HCV envelope genes, which encode envelope proteins E1 and E2, both antibody and lymphoproliferative responses against these proteins were induced. These responses were greatly enhanced by the codelivery of the GM-CSF gene. In particular, inoculation with a bicistronic plasmid that independently expressed the GM-CSF gene and the envelope genes in the same construct generated the highest antibody titers and significantly increased lymphoproliferative responses against these proteins. Moreover, strong antibody responses to homologous and heterologous hypervariable region 1 peptides were elicited in the immunized rats.

* Corresponding author. Mailing address: Department of Life Science, Pohang University of Science and Technology, San 31, Hyoja Dong, Pohang, 790-784 Korea. Phone: 82-562-279-2294. Fax: 82-562-279-5544. E-mail: ycsung{at}vision.postech.ac.kr.

Journal of Virology, October 1998, p. 8430-8436, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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