This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamashita, T.
Right arrow Articles by Yamazaki, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamashita, T.
Right arrow Articles by Yamazaki, S.

 Previous Article  |  Next Article 

Journal of Virology, October 1998, p. 8408-8412, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Complete Nucleotide Sequence and Genetic Organization of Aichi Virus, a Distinct Member of the Picornaviridae Associated with Acute Gastroenteritis in Humans

Teruo Yamashita,1,* Kenji Sakae,1 Hideaki Tsuzuki,1 Yasumoto Suzuki,1 Naohisa Ishikawa,1 Naokazu Takeda,2 Tatsuo Miyamura,2 and Shudo Yamazaki2

Department of Virology, Aichi Prefectural Institute of Public Health, Nagoya, Aichi 462-8576,1 and Department of Virology II, National Institute of Infectious Diseases, Tokyo 162-8640,2 Japan

Received 13 March 1998/Accepted 8 July 1998

The complete nucleotide sequence of a novel enteric virus, Aichi virus, associated with nonbacterial acute gastroenteritis in humans was determined. The Aichi virus genome proved to be a single-stranded positive-sense RNA molecule with 8,251 bases excluding a poly(A) tail; it contains a large open reading frame with 7,302 nucleotides that encodes a potential polyprotein precursor of 2,433 amino acids. The genome contains a 5' nontranslated region (NTR) with 712 bases and a 3' NTR with 240 bases followed by a poly(A) tail. The structure of the genome, VPg-5' NTR-leader protein-structural proteins-nonstructural proteins-3' NTR-poly(A), was found to be typical of a picornavirus. The VP0-VP3 and VP3-VP1 cleavage sites were determined to be Q-H and Q-T, respectively, by N-terminal amino acid sequence analyses using purified virion proteins. Possible cleavage sites, Q-G, Q-A, and Q-S, which cleave P2 and P3 polyproteins were found to be similar to those of picornaviruses. A dendrogram based on 3Dpol proteins indicated that Aichi virus is genetically distinct from the known six genera of picornaviruses including entero-, rhino-, cardio-, aphtho-, and hepatovirus and echovirus 22. Considering this together with other properties of the virus (T. Yamashita, S. Kobayashi, K. Sakae, S. Nakata, S. Chiba, Y. Ishihara, and S. Isomura, J. Infect. Dis. 164:954-957, 1991), we propose that Aichi virus be regarded as a new genus of the family Picornaviridae.

* Corresponding author. Mailing address: Department of Virology, Aichi Prefectural Institute of Public Health, 7-6, Nagare, Tsujimachi, Kita-ku, Nagoya, Aichi 462-8576, Japan. Phone: 81-52-911-3111. Fax: 81-52-913-3641. E-mail: tyamashita{at}hi-ho.ne.jp.

Journal of Virology, October 1998, p. 8408-8412, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Li, L., Victoria, J., Kapoor, A., Blinkova, O., Wang, C., Babrzadeh, F., Mason, C. J., Pandey, P., Triki, H., Bahri, O., Oderinde, B. S., Baba, M. M., Bukbuk, D. N., Besser, J. M., Bartkus, J. M., Delwart, E. L. (2009). A Novel Picornavirus Associated with Gastroenteritis. J. Virol. 83: 12002-12006 [Abstract] [Full Text]  
  • Sdiri-Loulizi, K., Hassine, M., Gharbi-Khelifi, H., Sakly, N., Chouchane, S., Guediche, M. N., Pothier, P., Aouni, M., Ambert-Balay, K. (2009). Detection and Genomic Characterization of Aichi Viruses in Stool Samples from Children in Monastir, Tunisia. J. Clin. Microbiol. 47: 2275-2278 [Abstract] [Full Text]  
  • Svraka, S., van der Veer, B., Duizer, E., Dekkers, J., Koopmans, M., Vennema, H. (2009). Novel Approach for Detection of Enteric Viruses To Enable Syndrome Surveillance of Acute Viral Gastroenteritis. J. Clin. Microbiol. 47: 1674-1679 [Abstract] [Full Text]  
  • Sasaki, J., Taniguchi, K. (2008). Aichi Virus 2A Protein Is Involved in Viral RNA Replication. J. Virol. 82: 9765-9769 [Abstract] [Full Text]  
  • Nagashima, S., Sasaki, J., Taniguchi, K. (2008). Interaction between Polypeptide 3ABC and the 5'-Terminal Structural Elements of the Genome of Aichi Virus: Implication for Negative-Strand RNA Synthesis. J. Virol. 82: 6161-6171 [Abstract] [Full Text]  
  • Ambert-Balay, K., Lorrot, M., Bon, F., Giraudon, H., Kaplon, J., Wolfer, M., Lebon, P., Gendrel, D., Pothier, P. (2008). Prevalence and Genetic Diversity of Aichi Virus Strains in Stool Samples from Community and Hospitalized Patients. J. Clin. Microbiol. 46: 1252-1258 [Abstract] [Full Text]  
  • Kapoor, A., Victoria, J., Simmonds, P., Wang, C., Shafer, R. W., Nims, R., Nielsen, O., Delwart, E. (2008). A Highly Divergent Picornavirus in a Marine Mammal. J. Virol. 82: 311-320 [Abstract] [Full Text]  
  • Pham, N. T. K., Khamrin, P., Nguyen, T. A., Kanti, D. S., Phan, T. G., Okitsu, S., Ushijima, H. (2007). Isolation and Molecular Characterization of Aichi Viruses from Fecal Specimens Collected in Japan, Bangladesh, Thailand, and Vietnam. J. Clin. Microbiol. 45: 2287-2288 [Abstract] [Full Text]  
  • Svraka, S., Duizer, E., Vennema, H., de Bruin, E., van der Veer, B., Dorresteijn, B., Koopmans, M. (2007). Etiological Role of Viruses in Outbreaks of Acute Gastroenteritis in The Netherlands from 1994 through 2005. J. Clin. Microbiol. 45: 1389-1394 [Abstract] [Full Text]  
  • Kim, M.-C., Kwon, Y.-K., Joh, S.-J., Lindberg, A. M., Kwon, J.-H., Kim, J.-H., Kim, S.-J. (2006). Molecular analysis of duck hepatitis virus type 1 reveals a novel lineage close to the genus Parechovirus in the family Picornaviridae.. J. Gen. Virol. 87: 3307-3316 [Abstract] [Full Text]  
  • Nagashima, S., Sasaki, J., Taniguchi, K. (2005). The 5'-Terminal Region of the Aichi Virus Genome Encodes cis-Acting Replication Elements Required for Positive- and Negative-Strand RNA Synthesis. J. Virol. 79: 6918-6931 [Abstract] [Full Text]  
  • Jokela, P., Joki-Korpela, P., Maaronen, M., Glumoff, V., Hyypia, T. (2005). Detection of Human Picornaviruses by Multiplex Reverse Transcription-PCR and Liquid Hybridization. J. Clin. Microbiol. 43: 1239-1245 [Abstract] [Full Text]  
  • Yamashita, T., Ito, M., Kabashima, Y., Tsuzuki, H., Fujiura, A., Sakae, K. (2003). Isolation and characterization of a new species of kobuvirus associated with cattle. J. Gen. Virol. 84: 3069-3077 [Abstract] [Full Text]  
  • Sasaki, J., Nagashima, S., Taniguchi, K. (2003). Aichi Virus Leader Protein Is Involved in Viral RNA Replication and Encapsidation. J. Virol. 77: 10799-10807 [Abstract] [Full Text]  
  • Johansson, E. S., Niklasson, B., Tesh, R. B., Shafren, D. R., Travassos da Rosa, A. P. A., Lindberg, A. M. (2003). Molecular characterization of M1146, an American isolate of Ljungan virus (LV) reveals the presence of a new LV genotype. J. Gen. Virol. 84: 837-844 [Abstract] [Full Text]  
  • Sasaki, J., Taniguchi, K. (2003). The 5'-End Sequence of the Genome of Aichi Virus, a Picornavirus, Contains an Element Critical for Viral RNA Encapsidation. J. Virol. 77: 3542-3548 [Abstract] [Full Text]  
  • Johansson, S., Niklasson, B., Maizel, J., Gorbalenya, A. E., Lindberg, A. M. (2002). Molecular Analysis of Three Ljungan Virus Isolates Reveals a New, Close-to-Root Lineage of the Picornaviridae with a Cluster of Two Unrelated 2A Proteins. J. Virol. 76: 8920-8930 [Abstract] [Full Text]  
  • Mari, J., Poulos, B. T., Lightner, D. V., Bonami, J.-R. (2002). Shrimp Taura syndrome virus: genomic characterization and similarity with members of the genus Cricket paralysis-like viruses. J. Gen. Virol. 83: 915-926 [Abstract] [Full Text]  
  • Oberste, M. S., Maher, K., Pallansch, M. A. (2002). Molecular Phylogeny and Proposed Classification of the Simian Picornaviruses. J. Virol. 76: 1244-1251 [Abstract] [Full Text]  
  • Yamashita, T., Ito, M., Tsuzuki, H., Sakae, K. (2001). Identification of Aichi Virus Infection by Measurement of Immunoglobulin Responses in an Enzyme-Linked Immunosorbent Assay. J. Clin. Microbiol. 39: 4178-4180 [Abstract] [Full Text]  
  • Sasaki, J., Kusuhara, Y., Maeno, Y., Kobayashi, N., Yamashita, T., Sakae, K., Takeda, N., Taniguchi, K. (2001). Construction of an Infectious cDNA Clone of Aichi Virus (a New Member of the Family Picornaviridae) and Mutational Analysis of a Stem-Loop Structure at the 5' End of the Genome. J. Virol. 75: 8021-8030 [Abstract] [Full Text]  
  • Oberste, M. S., Schnurr, D., Maher, K., al-Busaidy, S., Pallansch, M. A. (2001). Molecular identification of new picornaviruses and characterization of a proposed enterovirus 73 serotype. J. Gen. Virol. 82: 409-416 [Abstract] [Full Text]  
  • Kaku, Y., Sarai, A., Murakami, Y. (2001). Genetic reclassification of porcine enteroviruses. J. Gen. Virol. 82: 417-424 [Abstract] [Full Text]  
  • Imada, T., Yamaguchi, S., Mase, M., Tsukamoto, K., Kubo, M., Morooka, A. (2000). Avian Nephritis Virus (ANV) as a New Member of the Family Astroviridae and Construction of Infectious ANV cDNA. J. Virol. 74: 8487-8493 [Abstract] [Full Text]  
  • Yamashita, T., Sugiyama, M., Tsuzuki, H., Sakae, K., Suzuki, Y., Miyazaki, Y. (2000). Application of a Reverse Transcription-PCR for Identification and Differentiation of Aichi Virus, a New Member of the Picornavirus Family Associated with Gastroenteritis in Humans. J. Clin. Microbiol. 38: 2955-2961 [Abstract] [Full Text]  
  • Hughes, P. J., Stanway, G. (2000). The 2A proteins of three diverse picornaviruses are related to each other and to the H-rev107 family of proteins involved in the control of cell proliferation. J. Gen. Virol. 81: 201-207 [Abstract] [Full Text]  
  • Doherty, M., Todd, D., McFerran, N., Hoey, E. M. (1999). Sequence analysis of a porcine enterovirus serotype 1 isolate: relationships with other picornaviruses. J. Gen. Virol. 80: 1929-1941 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»