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Journal of Virology, October 1998, p. 8396-8402, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Specific and Independent Recognition of U3 and U5 att Sites by Human Immunodeficiency Virus Type 1 Integrase In Vivo

Takao Masuda,1,2,* Marcelo J. Kuroda,1,dagger and Shinji Harada1

Department of Biodefense and Medical Virology, Kumamoto University School of Medicine, Kumamoto,1 and Department of Immunotherapeutics, Medical Research Division, Tokyo Medical and Dental University, Tokyo,2 Japan

Received 13 May 1998/Accepted 10 July 1998

The retroviral attachment (att) sites at viral DNA ends are cis-acting regions essential for proviral integration. To investigate the sequence features of att important for human immunodeficiency virus type 1 (HIV-1) integration in vivo, we generated a series of 25 att mutants of HIV-1 by mutagenesis of the U3, U5, or both boundaries of att. Our results indicated that the terminal 11 or 12 bp of viral DNA are sufficient for specific recognition by HIV-1 integrase (IN) and suggested that IN might recognize each att site independently in vivo.

* Corresponding author. Mailing address: Department of Immunotherapeutics, Medical Research Division, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-Ku, Tokyo 113-8510, Japan. Phone: 81-3-5803-5799. Fax: 81-3-5803-0235. E-mail: tmasu.impt{at}med.tmd.ac.jp.

dagger Present address: Division of Viral Pathogenesis, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215.

Journal of Virology, October 1998, p. 8396-8402, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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