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Journal of Virology, October 1998, p. 8358-8361, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Two Novel Adenovirus Vector Systems Permitting Regulated Protein Expression in Gene Transfer Experiments

Magnus Molin,1 Maria C. Shoshan,2 Karin Öhman-Forslund,1,dagger Stig Linder,2 and Göran Akusjärvi1,*

Department of Medical Biochemistry and Microbiology, BMC, Uppsala University, S-751 23 Uppsala,1 and Radiumhemmet's Research Laboratory, Cancer Center Karolinska, Karolinska Institute and Hospital, S-171 76 Stockholm,2 Sweden

Received 4 May 1998/Accepted 15 July 1998

Two new adenovirus vector systems based on the tetracycline-regulated Tet-ON- (Gossen, M., et al., Science 268:1766-1769, 1995) and the RU 486-regulated progesterone antagonist (Wang, Y., et al., Proc. Natl. Acad. Sci. USA 91:8180-8184, 1994)-induced gene expression systems are described. We show that both systems permit a tight control of chloramphenicol acetyltransferase reporter gene expression in a variety of cell types, with induction levels of approximately 1,800-fold (Tet-ON system) and 600-fold (RU 486-regulated system), respectively. A significant advantage of our vector systems is that reporter protein expression can be adjusted over a wide range by varying the amount of inducer. The Tet-ON system is also shown to permit an efficient control of reporter gene expression in mice.

* Corresponding author. Mailing address: Department of Medical Biochemistry and Microbiology, BMC, Box 582, Uppsala University, S-751 23 Uppsala, Sweden. Phone: 46-18-471 4164. Fax: 46-18-50 98 76. E-mail: goran.akusjarvi{at}imim.uu.se.

dagger Present address: Department of Virology, The National Veterinary Institute, BMC, S-751 23 Uppsala, Sweden.

Journal of Virology, October 1998, p. 8358-8361, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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