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Journal of Virology, October 1998, p. 8349-8353, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Monitoring Retroviral RNA Dimerization In Vivo via Hammerhead Ribozyme Cleavage

Bijay K. Pal,1,2 Lisa Scherer,1 Laurie Zelby,1 Edouard Bertrand,1,3 and John J. Rossi1,*

Department of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, California 910101; Biological Sciences Department, California State Polytechnic University, Pomona, California 917682; and Institut de Genetique Moleculaire de Montpellier---CNRS, Montpellier, France3

Received 13 March 1998/Accepted 17 June 1998

We have used a strategy for colocalization of Psi (Psi )-tethered ribozymes and targets to demonstrate that Psi  sequences are capable of specific interaction in the cytoplasm of both packaging and nonpackaging cells. These results indicate that current in vitro dimerization models may have in vivo counterparts. The methodology used may be applied to further genetic analyses on Psi  domain interactions in vivo.

* Corresponding author. Mailing address: Department of Molecular Biology, Beckman Research Institute of the City of Hope, 1450 E. Duarte Rd., Duarte, CA 91010-3011. Phone: (626) 301-8360. Fax: (626) 301-8271. E-mail: jrossi{at}.coh.org.

Journal of Virology, October 1998, p. 8349-8353, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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