This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Urban, S.
Right arrow Articles by Schaller, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Urban, S.
Right arrow Articles by Schaller, H.

 Previous Article  |  Next Article 

Journal of Virology, October 1998, p. 8089-8097, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Avian Hepatitis B Virus Infection Is Initiated by the Interaction of a Distinct Pre-S Subdomain with the Cellular Receptor gp180

Stephan Urban, Klaus M. Breiner, Frank Fehler,dagger Ursula Klingmüller,Dagger and Heinz Schaller*

Zentrum für Molekulare Biologie, Universität Heidelberg, 69120 Heidelberg, Germany

Received 10 April 1998/Accepted 23 June 1998

Functionally relevant hepadnavirus-cell surface interactions were investigated with the duck hepatitis B virus (DHBV) animal model by using an in vitro infection competition assay. Recombinant DHBV pre-S polypeptides, produced in Escherichia coli, were shown to inhibit DHBV infection in a dose-dependent manner, indicating that monomeric pre-S chains were capable of interfering with virus-receptor interaction. Particle-associated pre-S was, however, 30-fold more active, suggesting that cooperative interactions enhance particle binding. An 85-amino-acid pre-S sequence, spanning about half of the DHBV pre-S chain, was characterized by deletion analysis as essential for maximal inhibition. Pre-S polypeptides from heron hepatitis B virus (HHBV) competed DHBV infection equally well despite a 50% difference in amino acid sequence and a much-reduced infectivity of HHBV for duck hepatocytes. These observations are taken to indicate (i) that the functionality of the DHBV pre-S subdomain, which interacts with the cellular receptor, is determined predominantly by a defined three-dimensional structure rather than by primary sequence elements; (ii) that cellular uptake of hepadnaviruses is a multistep process involving more than a single cellular receptor component; and (iii) that gp180, a cellular receptor candidate unable to discriminate between DHBV and HHBV, is a common component of the cellular receptor complex for avian hepadnaviruses.

* Corresponding author. Mailing address: ZMBH, University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany. Phone: 49 6221 546885. Fax: 49 6221 545893. E-mail: hshd{at}zmbh.uni-heidelberg.de.

dagger Present address: TAD Pharmazeutische Werke GmbH, 27472 Cuxhaven, Germany.

Dagger Present address: Max-Planck-Institut für Immunbiologie, 79108 Freiburg, Germany.

Journal of Virology, October 1998, p. 8089-8097, Vol. 72, No. 10
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Maenz, C., Chang, S.-F., Iwanski, A., Bruns, M. (2007). Entry of Duck Hepatitis B Virus into Primary Duck Liver and Kidney Cells after Discovery of a Fusogenic Region within the Large Surface Protein. J. Virol. 81: 5014-5023 [Abstract] [Full Text]  
  • Stoeckl, L., Funk, A., Kopitzki, A., Brandenburg, B., Oess, S., Will, H., Sirma, H., Hildt, E. (2006). Identification of a structural motif crucial for infectivity of hepatitis B viruses. Proc. Natl. Acad. Sci. USA 103: 6730-6734 [Abstract] [Full Text]  
  • Chojnacki, J., Anderson, D. A., Grgacic, E. V. L. (2005). A Hydrophobic Domain in the Large Envelope Protein Is Essential for Fusion of Duck Hepatitis B Virus at the Late Endosome. J. Virol. 79: 14945-14955 [Abstract] [Full Text]  
  • Barrera, A., Guerra, B., Notvall, L., Lanford, R. E. (2005). Mapping of the Hepatitis B Virus Pre-S1 Domain Involved in Receptor Recognition. J. Virol. 79: 9786-9798 [Abstract] [Full Text]  
  • Grgacic, E. V. L., Anderson, D. A. (2005). St, a Truncated Envelope Protein Derived from the S Protein of Duck Hepatitis B Virus, Acts as a Chaperone for the Folding of the Large Envelope Protein. J. Virol. 79: 5346-5352 [Abstract] [Full Text]  
  • Lin, L., Prassolov, A., Funk, A., Quinn, L., Hohenberg, H., Frolich, K., Newbold, J., Ludwig, A., Will, H., Sirma, H., Steinbach, F. (2005). Evidence from nature: interspecies spread of heron hepatitis B viruses. J. Gen. Virol. 86: 1335-1342 [Abstract] [Full Text]  
  • Guo, H., Mason, W. S., Aldrich, C. E., Saputelli, J. R., Miller, D. S., Jilbert, A. R., Newbold, J. E. (2005). Identification and Characterization of Avihepadnaviruses Isolated from Exotic Anseriformes Maintained in Captivity. J. Virol. 79: 2729-2742 [Abstract] [Full Text]  
  • Funk, A., Mhamdi, M., Lin, L., Will, H., Sirma, H. (2004). Itinerary of Hepatitis B Viruses: Delineation of Restriction Points Critical for Infectious Entry. J. Virol. 78: 8289-8300 [Abstract] [Full Text]  
  • Schildgen, O., Roggendorf, M., Lu, M. (2004). Identification of a glycosylation site in the woodchuck hepatitis virus preS2 protein and its role in protein trafficking. J. Gen. Virol. 85: 787-793 [Abstract] [Full Text]  
  • Urban, S., Gripon, P. (2002). Inhibition of Duck Hepatitis B Virus Infection by a Myristoylated Pre-S Peptide of the Large Viral Surface Protein. J. Virol. 76: 1986-1990 [Abstract] [Full Text]  
  • Sung, V. M.-H., Lai, M. M. C. (2002). Murine Retroviral Pseudotype Virus Containing Hepatitis B Virus Large and Small Surface Antigens Confers Specific Tropism for Primary Human Hepatocytes: a Potential Liver-Specific Targeting System. J. Virol. 76: 912-917 [Abstract] [Full Text]  
  • Breiner, K. M., Urban, S., Glass, B., Schaller, H. (2001). Envelope Protein-Mediated Down-Regulation of Hepatitis B Virus Receptor in Infected Hepatocytes. J. Virol. 75: 143-150 [Abstract] [Full Text]  
  • Schneider-Schaulies, J. (2000). Cellular receptors for viruses: links to tropism and pathogenesis. J. Gen. Virol. 81: 1413-1429 [Full Text]  
  • Grgacic, E. V. L., Schaller, H. (2000). A Metastable Form of the Large Envelope Protein of Duck Hepatitis B Virus: Low-pH Release Results in a Transition to a Hydrophobic, Potentially Fusogenic Conformation. J. Virol. 74: 5116-5122 [Abstract] [Full Text]  
  • Seeger, C., Mason, W. S. (2000). Hepatitis B Virus Biology. Microbiol. Mol. Biol. Rev. 64: 51-68 [Abstract] [Full Text]  
  • Breiner, K. M., Schaller, H. (2000). Cellular Receptor Traffic Is Essential for Productive Duck Hepatitis B Virus Infection. J. Virol. 74: 2203-2209 [Abstract] [Full Text]  
  • Ryu, C. J., Cho, D.-Y., Gripon, P., Kim, H. S., Guguen-Guillouzo, C., Hong, H. J. (2000). An 80-Kilodalton Protein That Binds to the Pre-S1 Domain of Hepatitis B Virus. J. Virol. 74: 110-116 [Abstract] [Full Text]  
  • Ganem, D. (1999). An advance in liver-specific gene delivery. Proc. Natl. Acad. Sci. USA 96: 11696-11697 [Full Text]  
  • Tong, S., Li, J., Wands, J. R. (1999). Carboxypeptidase D Is an Avian Hepatitis B Virus Receptor. J. Virol. 73: 8696-8702 [Abstract] [Full Text]  
  • Sunyach, C., Rollier, C., Robaczewska, M., Borel, C., Barraud, L., Kay, A., Trépo, C., Will, H., Cova, L. (1999). Residues Critical for Duck Hepatitis B Virus Neutralization Are Involved in Host Cell Interaction. J. Virol. 73: 2569-2575 [Abstract] [Full Text]  
  • Urban, S., Kruse, C., Multhaup, G. (1999). A Soluble Form of the Avian Hepatitis B Virus Receptor. BIOCHEMICAL CHARACTERIZATION AND FUNCTIONAL ANALYSIS OF THE RECEPTOR LIGAND COMPLEX. J. Biol. Chem. 274: 5707-5715 [Abstract] [Full Text]  
  • Rothmann, K., Schnolzer, M., Radziwill, G., Hildt, E., Moelling, K., Schaller, H. (1998). Host Cell-Virus Cross Talk: Phosphorylation of a Hepatitis B Virus Envelope Protein Mediates Intracellular Signaling. J. Virol. 72: 10138-10147 [Abstract] [Full Text]  
  • Breiner, K. M., Urban, S., Schaller, H. (1998). Carboxypeptidase D (gp180), a Golgi-Resident Protein, Functions in the Attachment and Entry of Avian Hepatitis B Viruses. J. Virol. 72: 8098-8104 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»