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J Virol, January 1998, p. 868-872, Vol. 72, No. 1
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Hepatitis G Virus Encodes Protease Activities Which Can Effect Processing of the Virus Putative Nonstructural Proteins

Alexander S. Belyaev,^1,* Susan Chong,¹ Alexander Novikov,¹ Ana Kongpachith,¹ Frank R. Masiarz,^2,3 Moon Lim,¹ and Jungsuh P. Kim¹

Genelabs Technologies, Inc., Redwood City, California 94063¹; Protein Structure Analysis Group, Chiron Corporation, Emeryville, California 94608-2916²; and Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California 94143³

Received 23 May 1997/Accepted 20 September 1997

The genome of a recently identified virus, hepatitis G virus (HGV), shows considerable homology to hepatitis C virus (HCV). Two HGV proteases similar to nonstructural proteins NS2 and NS3 of HCV were identified, and their cleavage site specificity was investigated. Amino acids essential for the protease activities were determined by mutation analysis. NS4A of HGV was demonstrated to be a cofactor for NS3-mediated proteolysis, with a region critical for activity residing between Leu₁₅₆₁ and Ala₁₅₉₈.

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^* Corresponding author. Mailing address: Genelabs Technologies, Inc., 505 Penobscot Dr., Redwood City, CA 94063. Phone: (415) 369-9500, ext. 566. Fax: (415) 368-0709. E-mail: alexb{at}genelabs.com.

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