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J Virol, January 1998, p. 817-822, Vol. 72, No. 1
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of Provirus Clones of Simian Foamy Virus Type 1

Ayalew Mergia* and Min Wu

Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610

Received 25 July 1997/Accepted 28 September 1997

We have cloned proviral DNA of simian foamy virus type 1 (SFV-1) from linear unintegrated DNA (pSFV-1). Transfection of pSFV-1 induces cytopathology in several cell lines with supernatants from the transfected cell culture containing infectious viral particles. Electron microscopy of the transfected cells revealed foamy virus particles. Deletion analysis of pSFV-1 indicated that the transcriptional transactivator (tas) gene located between env and the long terminal repeat is critical for virus replication, whereas the second open reading frame (ORF-2) in this region is dispensable. Although the tas and ORF-2 regions of foamy viruses have significantly diverged, the results presented here suggested that the gene products have similar functions. Recombinant pSFV-1 containing the cat gene was able to transduce the heterologous gene, indicating the utility of SFV-1 as a vector. An infectious clone of SFV-1 which is distantly related to the human foamy virus will provide a means to understand the biology of this unique group of viruses.

* Corresponding author. Mailing address: Department of Pathobiology, College of Veterinary Medicine, University of Florida, P.O. Box 100145, Gainesville, FL 32610-0145. Phone: (352) 392-4700, ext. 3939. Fax: (352) 392-9704. E-mail: aam{at}vetmed1.vetmed.ufl.edu.




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