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J Virol, January 1998, p. 811-816, Vol. 72, No. 1
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Minimal Requirement for a Lentivirus Vector Based on Human Immunodeficiency Virus Type 1

V. Narry Kim,1 Kyriacos Mitrophanous,1 Susan M. Kingsman,1,2 and Alan J. Kingsman1,2,*

Biochemistry Department, Oxford University, Oxford OX1 3QU,1 and Oxford BioMedica Limited, The Medawar Centre, The Oxford Science Park, Oxford OX4 4GA,2 United Kingdom

Received 7 July 1997/Accepted 28 September 1997

The use of human immunodeficiency virus vectors for gene therapy is hampered by concern over their safety. This concern might be ameliorated, in part, if the viral accessory genes and proteins could be eliminated from the vector genomes and particles. Here we describe a minimal vector system that is capable of transducing nondividing cells and which does not contain tat, vif, vpr, vpu, and nef.

* Corresponding author. Mailing address: Biochemistry Department, Oxford University, South Parks Rd., Oxford OX1 3QU, United Kingdom. Phone: 44-1865-275249. Fax: 44-1865-275259. E-mail: akingsmn{at}bioch.ox.ac.uk.




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