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J Virol, January 1998, p. 617-623, Vol. 72, No. 1
0022-538X/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Human Immunodeficiency Virus Type 1 Populations in Blood and Semen

Eric L. Delwart,1 James I. Mullins,2,* Phalguni Gupta,3 Gerald H. Learn Jr.,2 Mark Holodniy,4 David Katzenstein,5 Bruce D. Walker,6 and Mandaleshwar K. Singh3

Aaron Diamond AIDS Research Center and The Rockefeller University, New York, New York 100161; Departments of Microbiology and Medicine, University of Washington, Seattle, Washington 981952; Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, Pennsylvania 152613; Veterans Administration Hospital, Palo Alto, California 943044; Division of Infectious Diseases, Stanford University School of Medicine, Stanford, California 943055; and Infectious Disease Unit, Massachusetts General Hospital, Boston, Massachusetts 021146

Received 4 June 1997/Accepted 7 October 1997

Transmission of human immunodeficiency virus type 1 (HIV-1) usually results in outgrowth of viruses with macrophage-tropic phenotype and consensus non-syncytium-inducing (NSI) V3 loop sequences, despite the presence of virus with broader host range and the syncytium-inducing (SI) phenotype in the blood of many donors. We examined proviruses in contemporaneous peripheral blood mononuclear cells (PBMC) and nonspermatozoal semen mononuclear cells (NSMC) of five HIV-1-infected individuals to determine if this preferential outgrowth could be due to compartmentalization and thus preferential transmission of viruses of the NSI phenotype from the male genital tract. Phylogenetic reconstructions of ~700-bp sequences covering the second constant region through the fifth variable region (C2 to V5) of the viral envelope gene revealed distinct variant populations in the blood versus the semen in two patients with AIDS and in one asymptomatic individual (patient 613), whereas similar variant populations were found in both compartments in two other asymptomatic individuals. Variants with amino acids in the V3 loop that predict the SI phenotype were found in both AIDS patients and in patient 613; however, the distribution of these variants between the two compartments was not consistent. SI variants were found only in the PBMC of one AIDS patient but only in the NSMC of the other, while they were found in both compartments in patient 613. It is therefore unlikely that restriction of SI variants from the male genital tract accounts for the observed NSI transmission bias. Furthermore, no evidence for a semen-specific signature amino acid sequence was detected.

* Corresponding author. Mailing address: Departments of Microbiology & Medicine, University of Washington, Room K451, Seattle, WA 98195-7740. Phone: (206) 616-1851. Fax: (206) 616-1575. E-mail: jmullins{at}u.washington.edu.




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