This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tong, X. Articles by Howley, P. M. Search for Related Content PubMed PubMed Citation Articles by Tong, X. Articles by Howley, P. M.

 Previous Article  |  Next Article 

J. Virol., 01 1998, 476-482, Vol 72, No. 1
Copyright © 1998, American Society for Microbiology

Interaction of the bovine papillomavirus E6 protein with the clathrin adaptor complex AP-1

X Tong, W Boll, T Kirchhausen and PM Howley
Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.

The E6 gene of the bovine papillomavirus type 1 (BPV-1) is expressed in fibropapillomas caused by BPV-1 and in tissue culture cells transformed by BPV-1. It encodes one of the two major oncoproteins of BPV-1. In this study, we demonstrate an interaction between the BPV-1 E6 protein and AP-1, the TGN (trans-Golgi network)-specific clathrin adaptor complex. AP-1 is a four-subunit protein complex required for clathrin- mediated cellular transport from the TGN. The AP-1/E6 interaction was observed in vitro and in cells. The E6 binding site on AP-1 was mapped to the N-terminal trunk domain of the gamma subunit. BPV-1 E6 preferentially associated with membrane-bound AP-1 in cells but not with free cytosolic AP-1. BPV-1 E6 was further shown to be recruited to isolated Golgi membranes and to copurify with clathrin-coated vesicles. The recruitment of BPV-1 E6 to Golgi membranes was AP-1 independent, but the E6 interaction with AP-1 was required for its association with clathrin-coated vesicles. Furthermore, AP-1 proteins could compete with BPV-1 E6 for binding to Golgi membranes, suggesting that the recruitment of BPV-1 E6 and AP-1 to Golgi membranes involves a common factor. Taken together, our results suggest that cytosolic BPV-1 E6 is first recruited to the TGN, where it is then recognized by membrane- bound AP-1 and subsequently recruited into TGN-derived clathrin-coated vesicles. We propose that BPV-1 E6, through its interaction with AP-1, can affect cellular processes involving clathrin-mediated trafficking pathway.

This article has been cited by other articles:

• DeMasi, J., Chao, M. C., Kumar, A. S., Howley, P. M. (2007). Bovine Papillomavirus E7 Oncoprotein Inhibits Anoikis. J. Virol. 81: 9419-9425 [Abstract] [Full Text]  
• Nakatani, Y., Konishi, H., Vassilev, A., Kurooka, H., Ishiguro, K., Sawada, J.-i., Ikura, T., Korsmeyer, S. J., Qin, J., Herlitz, A. M. (2005). From the Cover: p600, a unique protein required for membrane morphogenesis and cell survival. Proc. Natl. Acad. Sci. USA 102: 15093-15098 [Abstract] [Full Text]  
• DeMasi, J., Huh, K.-W., Nakatani, Y., Munger, K., Howley, P. M. (2005). Bovine papillomavirus E7 transformation function correlates with cellular p600 protein binding. Proc. Natl. Acad. Sci. USA 102: 11486-11491 [Abstract] [Full Text]  
• Trinidad, J. C., Cohen, J. B. (2004). Neuregulin Inhibits Acetylcholine Receptor Aggregation in Myotubes. J. Biol. Chem. 279: 31622-31628 [Abstract] [Full Text]  
• Chambers, G., Ellsmore, V. A., O'Brien, P. M., Reid, S. W. J., Love, S., Campo, M. S., Nasir, L. (2003). Association of bovine papillomavirus with the equine sarcoid. J. Gen. Virol. 84: 1055-1062 [Abstract] [Full Text]  
• Hartmann-Stühler, C., Prange, R. (2001). Hepatitis B Virus Large Envelope Protein Interacts with {gamma}2-Adaptin, a Clathrin Adaptor-Related Protein. J. Virol. 75: 5343-5351 [Abstract] [Full Text]  
• Zimmermann, H., Koh, C.-H., Degenkolbe, R., O’Connor, M. J., Müller, A., Steger, G., Chen, J. J., Lui, Y., Androphy, E., Bernard, H.-U. (2000). Interaction with CBP/p300 enables the bovine papillomavirus type 1 E6 oncoprotein to downregulate CBP/p300-mediated transactivation by p53. J. Gen. Virol. 81: 2617-2623 [Abstract] [Full Text]  
• Pancio, H. A., Vander Heyden, N., Kosuri, K., Cresswell, P., Ratner, L. (2000). Interaction of Human Immunodeficiency Virus Type 2 Vpx and Invariant Chain. J. Virol. 74: 6168-6172 [Abstract] [Full Text]  
• Gao, Q., Kumar, A., Srinivasan, S., Singh, L., Mukai, H., Ono, Y., Wazer, D. E., Band, V. (2000). PKN Binds and Phosphorylates Human Papillomavirus E6 Oncoprotein. J. Biol. Chem. 275: 14824-14830 [Abstract] [Full Text]  
• Orzech, E., Schlessinger, K., Weiss, A., Okamoto, C. T., Aroeti, B. (1999). Interactions of the AP-1 Golgi Adaptor with the Polymeric Immunoglobulin Receptor and Their Possible Role in Mediating Brefeldin A-sensitive Basolateral Targeting from the trans-Golgi Network. J. Biol. Chem. 274: 2201-2215 [Abstract] [Full Text]  
• Chen, J. J., Hong, Y., Rustamzadeh, E., Baleja, J. D., Androphy, E. J. (1998). Identification of an alpha  Helical Motif Sufficient for Association with Papillomavirus E6. J. Biol. Chem. 273: 13537-13544 [Abstract] [Full Text]  
• Ploegh, H. L. (1998). Viral Strategies of Immune Evasion. Science 280: 248-253 [Abstract] [Full Text]