This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Battini, J. L.
Right arrow Articles by Heard, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Battini, J. L.
Right arrow Articles by Heard, J. M.

 Previous Article  |  Next Article 

J. Virol., 01 1998, 428-435, Vol 72, No. 1
Copyright © 1998, American Society for Microbiology

Definition of a 14-amino-acid peptide essential for the interaction between the murine leukemia virus amphotropic envelope glycoprotein and its receptor

JL Battini, O Danos and JM Heard
Laboratoire Retrovirus et Transfert Genetique, CNRS URA 1157, Institut Pasteur, Paris, France.

Hydrophilic loops in the receptor binding domain of the amphotropic murine leukemia virus (MLV) envelope glycoprotein (SU) are predicted and may participate in SU-receptor interactions. We have replaced five segments of 6 to 15 amino acids located in each of these regions with an 11-amino-acid tag from the vesicular stomatitis virus glycoprotein (VSV-G). Substitution was compatible with envelope processing, transport, and incorporation into virions. However, three substitution mutants showed a temperature-dependent phenotype, suggesting structural unstability. Accessibility of the tagging epitope for a monoclonal anti- VSV-G antibody was greater in oligomeric than in monomeric SUs when insertion was done in VRA, a domain essential for receptor recognition. In contrast, accessibility was independent of structural constraints when insertion was done in VRB, a domain playing an accessory role in receptor binding. Interaction with the amphotropic receptor was investigated by interference assay and study of binding and infection of target cells with MLV particles coated with the substituted envelopes. Envelope-receptor interaction was abolished when substitution was performed in a potential loop-forming segment located at the N-terminal half of VRA. Although interaction was affected to variable extents, depending on the substituted segment, other mutants conserved the ability to interact with the amphotropic receptor. These experiments indicate the 14-amino-acid segment between positions 50 and 64 of SU as an essential determinant of amphotropic-receptor recognition. They also show that a foreign linear epitope can be tolerated in several locations of the amphotropic SU receptor binding site, and this result has implications for the design of targeted retroviral vectors.


This article has been cited by other articles:

  • Verhoeyen, E., Cosset, F.-L. (2009). Engineering the Surface Glycoproteins of Lentiviral Vectors for Targeted Gene Transfer. CSH Protocols 2009: pdb.top59-pdb.top59 [Abstract] [Full Text]  
  • Sun, C., Zhang, B., Jin, J., Montelaro, R. C. (2008). Binding of equine infectious anemia virus to the equine lentivirus receptor-1 is mediated by complex discontinuous sequences in the viral envelope gp90 protein. J. Gen. Virol. 89: 2011-2019 [Abstract] [Full Text]  
  • Bahrami, S., Duch, M., Pedersen, F. S. (2004). Change of Tropism of SL3-2 Murine Leukemia Virus, Using Random Mutational Libraries. J. Virol. 78: 9343-9351 [Abstract] [Full Text]  
  • Lu, C.-W., O'Reilly, L., Roth, M. J. (2002). G100R Mutation within 4070A Murine Leukemia Virus Env Increases Virus Receptor Binding, Kinetics of Entry, and Viral Transduction Efficiency. J. Virol. 77: 739-743 [Abstract] [Full Text]  
  • Lauring, A. S., Cheng, H. H., Eiden, M. V., Overbaugh, J. (2002). Genetic and Biochemical Analyses of Receptor and Cofactor Determinants for T-Cell-Tropic Feline Leukemia Virus Infection. J. Virol. 76: 8069-8078 [Abstract] [Full Text]  
  • Farrell, K. B., Ting, Y.-T., Eiden, M. V. (2002). Fusion-Defective Gibbon Ape Leukemia Virus Vectors Can Be Rescued by Homologous but Not Heterologous Soluble Envelope Proteins. J. Virol. 76: 4267-4274 [Abstract] [Full Text]  
  • Overbaugh, J., Miller, A. D., Eiden, M. V. (2001). Receptors and Entry Cofactors for Retroviruses Include Single and Multiple Transmembrane-Spanning Proteins as well as Newly Described Glycophosphatidylinositol-Anchored and Secreted Proteins. Microbiol. Mol. Biol. Rev. 65: 371-389 [Abstract] [Full Text]  
  • Sugai, J., Eiden, M., Anderson, M. M., Van Hoeven, N., Meiering, C. D., Overbaugh, J. (2001). Identification of Envelope Determinants of Feline Leukemia Virus Subgroup B That Permit Infection and Gene Transfer to Cells Expressing Human Pit1 or Pit2. J. Virol. 75: 6841-6849 [Abstract] [Full Text]  
  • Lu, C.-W., Roth, M. J. (2001). Functional Characterization of the N Termini of Murine Leukemia Virus Envelope Proteins. J. Virol. 75: 4357-4366 [Abstract] [Full Text]  
  • Bachrach, E., Marin, M., Pelegrin, M., Karavanas, G., Piechaczyk, M. (2000). Efficient Cell Infection by Moloney Murine Leukemia Virus-Derived Particles Requires Minimal Amounts of Envelope Glycoprotein. J. Virol. 74: 8480-8486 [Abstract] [Full Text]  
  • Gwynn, S. R., Hankenson, F. C., Lauring, A. S., Rohn, J. L., Overbaugh, J. (2000). Feline Leukemia Virus Envelope Sequences That Affect T-Cell Tropism and Syncytium Formation Are Not Part of Known Receptor-Binding Domains. J. Virol. 74: 5754-5761 [Abstract] [Full Text]  
  • Tailor, C. S., Nouri, A., Kabat, D. (2000). A Comprehensive Approach to Mapping the Interacting Surfaces of Murine Amphotropic and Feline Subgroup B Leukemia Viruses with Their Cell Surface Receptors. J. Virol. 74: 237-244 [Abstract] [Full Text]  
  • O'Reilly, L., Roth, M. J. (2000). Second-Site Changes Affect Viability of Amphotropic/Ecotropic Chimeric Enveloped Murine Leukemia Viruses. J. Virol. 74: 899-913 [Abstract] [Full Text]  
  • Battini, J.-L., Rasko, J. E. J., Miller, A. D. (1999). A human cell-surface receptor for xenotropic and polytropic murine leukemia viruses: Possible role in G protein-coupled signal transduction. Proc. Natl. Acad. Sci. USA 96: 1385-1390 [Abstract] [Full Text]  
  • Han, J.-Y., Zhao, Y., Anderson, W. F., Cannon, P. M. (1998). Role of Variable Regions A and B in Receptor Binding Domain of Amphotropic Murine Leukemia Virus Envelope Protein. J. Virol. 72: 9101-9108 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»