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J. Virol., Jan 1998, 366-379, Vol 72, No. 1
Copyright © 1998, American Society for Microbiology

Defective growth correlates with reduced accumulation of a viral DNA replication protein after low-multiplicity infection by a human cytomegalovirus ie1 mutant

RF Greaves and ES Mocarski
Department of Medicine, Cambridge University Clinical School, Addenbrookes Hospital, United Kingdom. rfg@mole.bio.cam.ac.uk

To investigate the importance of the IE1 p72 regulatory protein during human cytomegalovirus replication, a recombinant virus unable to synthesize IE1 p72 was constructed. The Towne strain mutant CR208 lacked exon 4 of the major immediate-early gene and was isolated and complemented in an IE1-expressing immortalized human fibroblast line (ihfie1.3). Replication of CR208 in primary human fibroblasts was completed after an input multiplicity of 10 PFU/cell but was severely- impaired at 0.1 PFU/cell. CR208 formed plaques with lower efficiency on primary fibroblasts than on ihfiel.3 cells, and the relationship between the CR208 inoculum size and the resulting number of undersized plaques was nonlinear, indicating that multiple particles of CR208 were required to initiate lytic replication in a single primary fibroblast. After infection of primary fibroblasts with CR208 at 5 PFU/cell, a normal pattern of viral antigens was detected, although IE1 p72 was absent. During lower-multiplicity infections, IE2 protein was consistently detected at similar levels in a similar proportion of CR208-infected cells relative to the case for a Towne infection, but many fewer CR208-infected cells contained the ppUL44 polymerase accessory protein when evaluated at 24 or 48 h after infection. Furthermore, fibroblasts infected with CR208 at a low multiplicity failed to form viral DNA replication compartments, despite having expressed IE2 p86. These low-multiplicity growth and expression defects were corrected in two rescued derivatives of CR208 able to synthesize IE1 p72. One rescued virus (CR249) carried a deletion removing the large intron between exons 1 and 2 of the ie1-ie2 locus, revealing that this intron was dispensable for growth in cell culture.


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  • Xu, Y., Ahn, J.-H., Cheng, M., apRhys, C. M., Chiou, C.-J., Zong, J., Matunis, M. J., Hayward, G. S. (2001). Proteasome-Independent Disruption of PML Oncogenic Domains (PODs), but Not Covalent Modification by SUMO-1, Is Required for Human Cytomegalovirus Immediate-Early Protein IE1 To Inhibit PML-Mediated Transcriptional Repression. J. Virol. 75: 10683-10695 [Abstract] [Full Text]  
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  • McSharry, B. P., Jones, C. J., Skinner, J. W., Kipling, D., Wilkinson, G. W. G. (2001). Human telomerase reverse transcriptase-immortalized MRC-5 and HCA2 human fibroblasts are fully permissive for human cytomegalovirus. J. Gen. Virol. 82: 855-863 [Abstract] [Full Text]  
  • Presti, R. M., Popkin, D. L., Connick, M., Paetzold, S., Virgin, H. W. , IV (2001). Novel Cell Type-specific Antiviral Mechanism of Interferon {{gamma}} Action in Macrophages. JEM 193: 483-496 [Abstract] [Full Text]  
  • Meier, J. L. (2001). Reactivation of the Human Cytomegalovirus Major Immediate-Early Regulatory Region and Viral Replication in Embryonal NTera2 Cells: Role of Trichostatin A, Retinoic Acid, and Deletion of the 21-Base-Pair Repeats and Modulator. J. Virol. 75: 1581-1593 [Abstract] [Full Text]  
  • Marchini, A., Liu, H., Zhu, H. (2001). Human Cytomegalovirus with IE-2 (UL122) Deleted Fails To Express Early Lytic Genes. J. Virol. 75: 1870-1878 [Abstract] [Full Text]  
  • Bresnahan, W. A., Shenk, T. E. (2000). UL82 virion protein activates expression of immediate early viral genes in human cytomegalovirus-infected cells. Proc. Natl. Acad. Sci. USA 97: 14506-14511 [Abstract] [Full Text]  
  • Bell, P., Lieberman, P. M., Maul, G. G. (2000). Lytic but Not Latent Replication of Epstein-Barr Virus Is Associated with PML and Induces Sequential Release of Nuclear Domain 10 Proteins. J. Virol. 74: 11800-11810 [Abstract] [Full Text]  
  • Chen, J., Stinski, M. F. (2000). Activation of Transcription of the Human Cytomegalovirus Early UL4 Promoter by the Ets Transcription Factor Binding Element. J. Virol. 74: 9845-9857 [Abstract] [Full Text]  
  • White, K. L., Slobedman, B., Mocarski, E. S. (2000). Human Cytomegalovirus Latency-Associated Protein pORF94 Is Dispensable for Productive and Latent Infection. J. Virol. 74: 9333-9337 [Abstract] [Full Text]  
  • Murphy, E. A., Streblow, D. N., Nelson, J. A., Stinski, M. F. (2000). The Human Cytomegalovirus IE86 Protein Can Block Cell Cycle Progression after Inducing Transition into the S Phase of Permissive Cells. J. Virol. 74: 7108-7118 [Abstract] [Full Text]  
  • Meier, J. L., Pruessner, J. A. (2000). The Human Cytomegalovirus Major Immediate-Early Distal Enhancer Region Is Required for Efficient Viral Replication and Immediate-Early Gene Expression. J. Virol. 74: 1602-1613 [Abstract] [Full Text]  
  • Ahn, J.-H., Jang, W.-J., Hayward, G. S. (1999). The Human Cytomegalovirus IE2 and UL112-113 Proteins Accumulate in Viral DNA Replication Compartments That Initiate from the Periphery of Promyelocytic Leukemia Protein-Associated Nuclear Bodies (PODs or ND10). J. Virol. 73: 10458-10471 [Abstract] [Full Text]  
  • Lundquist, C. A., Meier, J. L., Stinski, M. F. (1999). A Strong Negative Transcriptional Regulatory Region between the Human Cytomegalovirus UL127 Gene and the Major Immediate-Early Enhancer. J. Virol. 73: 9039-9052 [Abstract] [Full Text]  
  • Borst, E.-M., Hahn, G., Koszinowski, U. H., Messerle, M. (1999). Cloning of the Human Cytomegalovirus (HCMV) Genome as an Infectious Bacterial Artificial Chromosome in Escherichia coli: a New Approach for Construction of HCMV Mutants. J. Virol. 73: 8320-8329 [Abstract] [Full Text]  
  • Jacobson, J. G., Renau, T. E., Nassiri, M. R., Sweier, D. G., Breitenbach, J. M., Townsend, L. B., Drach, J. C. (1999). Nonnucleoside Pyrrolopyrimidines with a Unique Mechanism of Action against Human Cytomegalovirus. Antimicrob. Agents Chemother. 43: 1888-1894 [Abstract] [Full Text]  
  • Müller, S., Dejean, A. (1999). Viral Immediate-Early Proteins Abrogate the Modification by SUMO-1 of PML and Sp100 Proteins, Correlating with Nuclear Body Disruption. J. Virol. 73: 5137-5143 [Abstract] [Full Text]  
  • Johnson, R., Yurochko, A., Poma, E., Zhu, L, Huang, E. (1999). Domain mapping of the human cytomegalovirus IE1-72 and cellular p107 protein--protein interaction and the possible functional consequences. J. Gen. Virol. 80: 1293-1303 [Abstract]  
  • Kurz, S. K., Rapp, M., Steffens, H.-P., Grzimek, N. K. A., Schmalz, S., Reddehase, M. J. (1999). Focal Transcriptional Activity of Murine Cytomegalovirus during Latency in the Lungs. J. Virol. 73: 482-494 [Abstract] [Full Text]  
  • Lashmit, P. E., Stinski, M. F., Murphy, E. A., Bullock, G. C. (1998). A cis Repression Sequence Adjacent to the Transcription Start Site of the Human Cytomegalovirus US3 Gene Is Required To Down Regulate Gene Expression at Early and Late Times after Infection. J. Virol. 72: 9575-9584 [Abstract] [Full Text]  
  • Angulo, A., Messerle, M., Koszinowski, U. H., Ghazal, P. (1998). Enhancer Requirement for Murine Cytomegalovirus Growth and Genetic Complementation by the Human Cytomegalovirus Enhancer. J. Virol. 72: 8502-8509 [Abstract] [Full Text]  
  • Ahn, J.-H., Brignole, E. J. III, Hayward, G. S. (1998). Disruption of PML Subnuclear Domains by the Acidic IE1 Protein of Human Cytomegalovirus Is Mediated through Interaction with PML and May Modulate a RING Finger-Dependent Cryptic Transactivator Function of PML. Mol. Cell. Biol. 18: 4899-4913 [Abstract] [Full Text]  
  • Prosch, S., Heine, A.-K., Volk, H.-D., Kruger, D. H. (2001). CCAAT/Enhancer-binding Proteins alpha and beta Negatively Influence the Capacity of Tumor Necrosis Factor alpha to Up-regulate the Human Cytomegalovirus IE1/2 Enhancer/Promoter by Nuclear Factor kappa B during Monocyte Differentiation. J. Biol. Chem. 276: 40712-40720 [Abstract] [Full Text]  
  • Trang, P., Lee, M., Nepomuceno, E., Kim, J., Zhu, H., Liu, F. (2000). Effective inhibition of human cytomegalovirus gene expression and replication by a ribozyme derived from the catalytic RNA subunit of RNase P from Escherichia coli. Proc. Natl. Acad. Sci. USA 97: 5812-5817 [Abstract] [Full Text]  


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