This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, W.
Right arrow Articles by Flint, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, W.
Right arrow Articles by Flint, S. J.

 Previous Article  |  Next Article 

J. Virol., Jan 1998, 225-235, Vol 72, No. 1
Copyright © 1998, American Society for Microbiology

The tripartite leader sequence of subgroup C adenovirus major late mRNAs can increase the efficiency of mRNA export

W Huang and SJ Flint
Department of Molecular Biology, Princeton University, New Jersey 08544, USA.

The subgroup C human adenoviruses induce selective export of newly synthesized viral mRNA from the nucleus to the cytoplasm, with concomitant inhibition of export of the majority of cellular mRNA species. Such posttranscriptional regulation of viral and cellular gene expression in infected cells requires viral E1B and E4 proteins. To facilitate the investigation of parameters that govern selective export in adenovirus-infected cells, we constructed a marked human beta-actin minigene under the control of the glucocorticoid-inducible enhancer- promoter of mouse mammary tumor virus and introduced it into the left end of the adenovirus type 5 (Ad5) genome. Transcription of this reporter gene (designated MA) as well as of a sibling, which differed only in the inclusion of a cDNA copy of the Ad2 major late tripartite leader sequence upstream of beta-actin sequences (termed MtplA), in recombinant virus-infected cells was strictly dependent on the addition of dexamethasone to the medium. When transcription of the MA gene was induced during the late phase of infection, newly synthesized MA RNA entered the cytoplasm. These transcripts, which contain no viral sequences, therefore reproduce the behavior of exceptional cellular mRNA species observed when transcription of their genes is activated during the late phase of infection (U.-C. Yang, W. Huang, and S. J. Flint, J. Virol. 70:4071-4080, 1996). Unexpectedly, however, higher concentrations of newly synthesized RNA accumulated in the cytoplasm when the tripartite leader sequence was present in the reporter RNA, despite equal rates of transcription of the two reporter genes. Examination of the partitioning of both newly synthesized and steady- state populations of MA and MtplA RNAs between nuclear and cytoplasmic compartments indicated that the tripartite leader sequence did not increase RNA stability in the cytoplasm. Comparison of nuclear and cytoplasmic reporter RNA species by Northern blotting, primer extension, and reverse transcription-PCR provided no evidence for altered processing induced by the tripartite leader sequence. We therefore conclude that the tripartite leader sequence, long known to facilitate the translation of mRNAs during the late phase of adenovirus infection, can also modulate mRNA export from the nucleus.


This article has been cited by other articles:

  • Spurgeon, M. E., Ornelles, D. A. (2009). The Adenovirus E1B 55-Kilodalton and E4 Open Reading Frame 6 Proteins Limit Phosphorylation of eIF2{alpha} during the Late Phase of Infection. J. Virol. 83: 9970-9982 [Abstract] [Full Text]  
  • Szelechowski, M., Fournier, A., Richardson, J., Eloit, M., Klonjkowski, B. (2009). Functional organization of the major late transcriptional unit of canine adenovirus type 2. J. Gen. Virol. 90: 1215-1223 [Abstract] [Full Text]  
  • Woo, J. L., Berk, A. J. (2007). Adenovirus Ubiquitin-Protein Ligase Stimulates Viral Late mRNA Nuclear Export. J. Virol. 81: 575-587 [Abstract] [Full Text]  
  • Xi, Q., Cuesta, R., Schneider, R. J. (2005). Regulation of Translation by Ribosome Shunting through Phosphotyrosine-Dependent Coupling of Adenovirus Protein 100k to Viral mRNAs. J. Virol. 79: 5676-5683 [Abstract] [Full Text]  
  • Farley, D. C., Brown, J. L., Leppard, K. N. (2004). Activation of the Early-Late Switch in Adenovirus Type 5 Major Late Transcription Unit Expression by L4 Gene Products. J. Virol. 78: 1782-1791 [Abstract] [Full Text]  
  • Huang, W., Flint, S. J. (2003). Unusual Properties of Adenovirus E2E Transcription by RNA Polymerase III. J. Virol. 77: 4015-4024 [Abstract] [Full Text]  
  • Weigel, S., Dobbelstein, M. (2000). The Nuclear Export Signal within the E4orf6 Protein of Adenovirus Type 5 Supports Virus Replication and Cytoplasmic Accumulation of Viral mRNA. J. Virol. 74: 764-772 [Abstract] [Full Text]  
  • Feigenblum, D., Walker, R., Schneider, R. J. (1998). Adenovirus Induction of an Interferon-Regulatory Factor during Entry into the Late Phase of Infection. J. Virol. 72: 9257-9266 [Abstract] [Full Text]  
  • Gustin, K. E., Imperiale, M. J. (1998). Encapsidation of Viral DNA Requires the Adenovirus L1 52/55-Kilodalton Protein. J. Virol. 72: 7860-7870 [Abstract] [Full Text]  
  • Gabler, S., Schutt, H., Groitl, P., Wolf, H., Shenk, T., Dobner, T. (1998). E1B 55-Kilodalton-Associated Protein: a Cellular Protein with RNA-Binding Activity Implicated in Nucleocytoplasmic Transport of Adenovirus and Cellular mRNAs. J. Virol. 72: 7960-7971 [Abstract] [Full Text]  
  • Molin, M., Shoshan, M. C., Ohman-Forslund, K., Linder, S., Akusjarvi, G. (1998). Two Novel Adenovirus Vector Systems Permitting Regulated Protein Expression in Gene Transfer Experiments. J. Virol. 72: 8358-8361 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»