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J. Virol., 01 1998, 113-120, Vol 72, No. 1
TA Reinhart, MJ Rogan, AM Amedee, M Murphey-Corb, DM Rausch, LE Eiden and AT Haase
Genetically distinct lentiviruses constitute a quasispecies population that
can evolve in response to selective forces. To move beyond characterization
of the population as a whole to the behavior of individual members, we
devised an in situ hybridization approach that uses genotype-specific
probes. We used probes that detect simian immunodeficiency viruses (SIV)
that differ in sequence in the V1 region of the surface envelope
glycoprotein (env) gene to investigate the replication and cellular
tropisms of four viral variants in the tissues of infected rhesus macaques.
We found that the V1 genotypic variants replicated in spatially defined
patterns and to different extents at each anatomic site. The two variants
that replicated most extensively in animals with AIDS were detected in both
macrophages and T lymphocytes in tissues. By extension of this approach, it
will be possible to investigate the role of individual lentiviruses in a
quasispecies in pathogenesis and to evaluate the effects of antiviral or
immunotherapeutic treatment on select members of a quasispecies.
Copyright © 1998, American Society for Microbiology
Tracking members of the simian immunodeficiency virus deltaB670 quasispecies population in vivo at single-cell resolution
Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455, USA.
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