This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ensser, A. Articles by Fleckenstein, B. Search for Related Content PubMed PubMed Citation Articles by Ensser, A. Articles by Fleckenstein, B.

 Previous Article  |  Next Article 

J. Virol., 09 1997, 6517-6525, Vol 71, No. 9
Copyright © 1997, American Society for Microbiology

Primary structure of the alcelaphine herpesvirus 1 genome

A Ensser, R Pflanz and B Fleckenstein
Institut fur Klinische und Molekulare Virologie der Universitat, Erlangen-Nuberg, Germany. ensser@viro.med.uni-erlangen.de

Alcelaphine herpesvirus 1 (AHV-1) causes wildebeest-associated malignant catarrhal fever, a lymphoproliferative syndrome in ungulate species other than the natural host. Based on biological properties and limited structural data, it has been classified as a member of the genus Rhadinovirus of the subfamily Gammaherpes-virinae. Here, we report on cloning and structural analysis of the complete genome of AHV- 1 C500. The low GC content DNA (L-DNA) region of the genome consists of 130,608 bp with low (46.17%) GC content and marked suppression of CpG dinucleotide frequency. Like in herpesvirus saimiri, the prototype of the rhadinoviruses, the L-DNA is flanked by approximately 20 to 25 GC- rich (71.83%) high GC content DNA (H-DNA) repeats of 1,113 to 1,118 nucleotides. The analysis of the L-DNA sequence revealed 70 open reading frames (ORFs), 61 of which showed homology to other herpesviruses. The conserved ORFs are arranged in four blocks collinear to other Rhadinovirus genomes. These gene blocks are flanked by nonconserved regions containing ORFs without similarities to known herpesvirus genes. Notably, a spliced reading frame with a coding capacity for a 199-amino-acid protein is located in a position homologous to the transforming genes of herpesvirus saimiri at the left end of the L-DNA. A gene with homology to the semaphorin family is located adjacent to this. Despite common biological and epidemiological properties, AHV-1 differs significantly from herpesvirus saimiri with regard to cell homologous genes, probably using a different set of effector proteins to achieve a similar T-lymphocyte-transforming phenotype.

This article has been cited by other articles:

• Jayawardane, G., Russell, G. C., Thomson, J., Deane, D., Cox, H., Gatherer, D., Ackermann, M., Haig, D. M., Stewart, J. P. (2008). A captured viral interleukin 10 gene with cellular exon structure. J. Gen. Virol. 89: 2447-2455 [Abstract] [Full Text]  
• Dry, I., Haig, D. M., Inglis, N. F., Imrie, L., Stewart, J. P., Russell, G. C. (2008). Proteomic Analysis of Pathogenic and Attenuated Alcelaphine Herpesvirus 1. J. Virol. 82: 5390-5397 [Abstract] [Full Text]  
• Boudry, C., Markine-Goriaynoff, N., Delforge, C., Springael, J.-Y., de Leval, L., Drion, P., Russell, G., Haig, D. M., Vanderplasschen, A. F., Dewals, B. (2007). The A5 gene of alcelaphine herpesvirus 1 encodes a constitutively active G-protein-coupled receptor that is non-essential for the induction of malignant catarrhal fever in rabbits. J. Gen. Virol. 88: 3224-3233 [Abstract] [Full Text]  
• Hart, J., Ackermann, M., Jayawardane, G., Russell, G., Haig, D. M., Reid, H., Stewart, J. P. (2007). Complete sequence and analysis of the ovine herpesvirus 2 genome. J. Gen. Virol. 88: 28-39 [Abstract] [Full Text]  
• Taus, N. S., Herndon, D. R., Traul, D. L., Stewart, J. P., Ackermann, M., Li, H., Knowles, D. P., Lewis, G. S., Brayton, K. A. (2007). Comparison of ovine herpesvirus 2 genomes isolated from domestic sheep (Ovis aries) and a clinically affected cow (Bos bovis). J. Gen. Virol. 88: 40-45 [Abstract] [Full Text]  
• Dewals, B., Boudry, C., Gillet, L., Markine-Goriaynoff, N., de Leval, L., Haig, D. M., Vanderplasschen, A. (2006). Cloning of the genome of Alcelaphine herpesvirus 1 as an infectious and pathogenic bacterial artificial chromosome.. J. Gen. Virol. 87: 509-517 [Abstract] [Full Text]  
• McGeoch, D. J., Gatherer, D., Dolan, A. (2005). On phylogenetic relationships among major lineages of the Gammaherpesvirinae. J. Gen. Virol. 86: 307-316 [Abstract] [Full Text]  
• Verma, S. C., Robertson, E. S. (2003). ORF73 of Herpesvirus Saimiri Strain C488 Tethers the Viral Genome to Metaphase Chromosomes and Binds to cis-Acting DNA Sequences in the Terminal Repeats. J. Virol. 77: 12494-12506 [Abstract] [Full Text]  
• Rosbottom, J., Dalziel, R. G., Reid, H. W., Stewart, J. P. (2002). Ovine herpesvirus 2 lytic cycle replication and capsid production. J. Gen. Virol. 83: 2999-3002 [Abstract] [Full Text]  
• Klieforth, R., Maalouf, G., Stalis, I., Terio, K., Janssen, D., Schrenzel, M. (2002). Malignant Catarrhal Fever-Like Disease in Barbary Red Deer (Cervus elaphus barbarus) Naturally Infected with a Virus Resembling Alcelaphine Herpesvirus 2. J. Clin. Microbiol. 40: 3381-3390 [Abstract] [Full Text]  
• Coulter, L. J., Reid, H. W. (2002). Isolation and expression of three open reading frames from ovine herpesvirus-2. J. Gen. Virol. 83: 533-543 [Abstract] [Full Text]  
• Dunowska, M., Letchworth, G. J., Collins, J. K., DeMartini, J. C. (2001). Ovine herpesvirus-2 glycoprotein B sequences from tissues of ruminant malignant catarrhal fever cases and healthy sheep are highly conserved. J. Gen. Virol. 82: 2785-2790 [Abstract] [Full Text]  
• Ehlers, B., Burkhardt, S., Goltz, M., Bergmann, V., Ochs, A., Weiler, H., Hentschke, J. (2001). Genetic and ultrastructural characterization of a European isolate of the fatal endotheliotropic elephant herpesvirus. J. Gen. Virol. 82: 475-482 [Abstract] [Full Text]  
• Hör, S., Ensser, A., Reiss, C., Ballmer-Hofer, K., Biesinger, B. (2001). Herpesvirus saimiri protein StpB associates with cellular Src. J. Gen. Virol. 82: 339-344 [Abstract] [Full Text]  
• Zimmermann, W., Broll, H., Ehlers, B., Buhk, H.-J., Rosenthal, A., Goltz, M. (2001). Genome Sequence of Bovine Herpesvirus 4, a Bovine Rhadinovirus, and Identification of an Origin of DNA Replication. J. Virol. 75: 1186-1194 [Abstract] [Full Text]  
• Hussy, D., Stauber, N., Leutenegger, C. M., Rieder, S., Ackermann, M. (2001). Quantitative Fluorogenic PCR Assay for Measuring Ovine Herpesvirus 2 Replication in Sheep. CVI 8: 123-128 [Abstract] [Full Text]  
• Nicholas, J (2000). Evolutionary aspects of oncogenic herpesviruses. Mol. Pathol. 53: 222-237 [Abstract] [Full Text]  
• Schwam, D. R., Luciano, R. L., Mahajan, S. S., Wong, L., Wilson, A. C. (2000). Carboxy Terminus of Human Herpesvirus 8 Latency-Associated Nuclear Antigen Mediates Dimerization, Transcriptional Repression, and Targeting to Nuclear Bodies. J. Virol. 74: 8532-8540 [Abstract] [Full Text]  
• Li, H., Dyer, N., Keller, J., Crawford, T. B. (2000). Newly Recognized Herpesvirus Causing Malignant Catarrhal Fever in White-Tailed Deer (Odocoileus virginianus). J. Clin. Microbiol. 38: 1313-1318 [Abstract] [Full Text]  
• Albrecht, J.-C. (2000). Primary Structure of the Herpesvirus Ateles Genome. J. Virol. 74: 1033-1037 [Abstract] [Full Text]  
• Platt, G. M., Simpson, G. R., Mittnacht, S., Schulz, T. F. (1999). Latent Nuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus Interacts with RING3, a Homolog of the Drosophila Female Sterile Homeotic (fsh) Gene. J. Virol. 73: 9789-9795 [Abstract] [Full Text]  
• Camarda, G., Spinetti, G., Bernardini, G., Mair, C., Davis-Poynter, N., Capogrossi, M. C., Napolitano, M. (1999). The Equine Herpesvirus 2 E1 Open Reading Frame Encodes a Functional Chemokine Receptor. J. Virol. 73: 9843-9848 [Abstract] [Full Text]  
• Ulrich, S., Goltz, M., Ehlers, B. (1999). Characterization of the DNA polymerase loci of the novel porcine lymphotropic herpesviruses 1 and 2 in domestic and feral pigs. J. Gen. Virol. 80: 3199-3205 [Abstract] [Full Text]  
• Bello, L. J., Davison, A. J., Glenn, M. A., Whitehouse, A., Rethmeier, N., Schulz, T. F., Barklie Clements, J. (1999). The human herpesvirus-8 ORF 57 gene and its properties. J. Gen. Virol. 80: 3207-3215 [Abstract] [Full Text]  
• Albrecht, J.-C., Friedrich, U., Kardinal, C., Koehn, J., Fleckenstein, B., Feller, S. M., Biesinger, B. (1999). Herpesvirus Ateles Gene Product Tio Interacts with Nonreceptor Protein Tyrosine Kinases. J. Virol. 73: 4631-4639 [Abstract] [Full Text]  
• Searles, R. P., Bergquam, E. P., Axthelm, M. K., Wong, S. W. (1999). Sequence and Genomic Analysis of a Rhesus Macaque Rhadinovirus with Similarity to Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus 8. J. Virol. 73: 3040-3053 [Abstract] [Full Text]  
• Plafker, S. M., Gibson, W. (1998). Cytomegalovirus Assembly Protein Precursor and Proteinase Precursor Contain Two Nuclear Localization Signals That Mediate Their Own Nuclear Translocation and That of the Major Capsid Protein. J. Virol. 72: 7722-7732 [Abstract] [Full Text]  
• Xu, X., Ng, S., Wu, Z.-L., Nguyen, D., Homburger, S., Seidel-Dugan, C., Ebens, A., Luo, Y. (1998). Human Semaphorin K1 Is Glycosylphosphatidylinositol-linked and Defines a New Subfamily of Viral-related Semaphorins. J. Biol. Chem. 273: 22428-22434 [Abstract] [Full Text]  
• Derfuss, T., Fickenscher, H., Kraft, M. S., Henning, G., Lengenfelder, D., Fleckenstein, B., Meinl, E. (1998). Antiapoptotic Activity of the Herpesvirus Saimiri-Encoded Bcl-2 Homolog: Stabilization of Mitochondria and Inhibition of Caspase-3-Like Activity. J. Virol. 72: 5897-5904 [Abstract] [Full Text]  
• Rovnak, J., Quackenbush, S. L., Reyes, R. A., Baines, J. D., Parrish, C. R., Casey, J. W. (1998). Detection of a Novel Bovine Lymphotropic Herpesvirus. J. Virol. 72: 4237-4242 [Abstract] [Full Text]  
• Neipel, F., Albrecht, J.-C., Fleckenstein, B. (1998). Human Herpesvirus 8--the First Human Rhadinovirus. J Natl Cancer Inst Monogr 1998: 73-77 [Abstract] [Full Text]