This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Van Lint, C.
Right arrow Articles by Verdin, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Van Lint, C.
Right arrow Articles by Verdin, E.

 Previous Article  |  Next Article 

J. Virol., 08 1997, 6113-6127, Vol 71, No. 8
Copyright © 1997, American Society for Microbiology

Transcription factor binding sites downstream of the human immunodeficiency virus type 1 transcription start site are important for virus infectivity

C Van Lint, CA Amella, S Emiliani, M John, T Jie and E Verdin
The Picower Institute for Medical Research, Manhasset, New York 11030, USA.

When transcriptionally active, the human immunodeficiency virus (HIV) promoter contains a nucleosome-free region encompassing both the promoter/enhancer region and a large region (255 nucleotides [nt]) downstream of the transcription start site. We have previously identified new binding sites for transcription factors downstream of the transcription start site (nt 465 to 720): three AP-1 sites (I, II, and III), an AP3-like motif (AP3-L), a downstream binding factor (DBF) site, and juxtaposed Sp1 sites. Here, we show that the DBF site is an interferon-responsive factor (IRF) binding site and that the AP3-L motif binds the T-cell-specific factor NF-AT. Mutations that abolish the binding of each factor to its cognate site are introduced in an infectious HIV-1 molecular clone to study their effect on HIV-1 transcription and replication. Individual mutation of the DBF or AP3-L site as well as the double mutation AP-1(III)/AP3-L did not affect HIV- 1 replication compared to that of the wild-type virus. In contrast, proviruses carrying mutations in the Sp1 sites were totally defective in terms of replication. Virus production occurred with slightly delayed kinetics for viruses containing combined mutations in the AP- 1(III), AP3-L, and DBF sites and in the AP3-L and DBF-sites, whereas viruses mutated in the AP-1(I,II,III) and AP3-L sites and in the AP- 1(I,II,III), AP3-L, and DBF sites exhibited a severely defective replicative phenotype. No RNA-packaging defect could be measured for any of the mutant viruses as determined by quantification of their HIV genomic RNA. Measurement of the transcriptional activity of the HIV-1 promoter after transient transfection of the HIV-1 provirus DNA or of long terminal repeat-luciferase constructs showed a positive correlation between the transcriptional and the replication defects for most mutants.


This article has been cited by other articles:

  • Hache, G., Abbink, T. E. M., Berkhout, B., Harris, R. S. (2009). Optimal Translation Initiation Enables Vif-Deficient Human Immunodeficiency Virus Type 1 To Escape Restriction by APOBEC3G. J. Virol. 83: 5956-5960 [Abstract] [Full Text]  
  • Sgarbanti, M., Remoli, A. L., Marsili, G., Ridolfi, B., Borsetti, A., Perrotti, E., Orsatti, R., Ilari, R., Sernicola, L., Stellacci, E., Ensoli, B., Battistini, A. (2008). IRF-1 Is Required for Full NF-{kappa}B Transcriptional Activity at the Human Immunodeficiency Virus Type 1 Long Terminal Repeat Enhancer. J. Virol. 82: 3632-3641 [Abstract] [Full Text]  
  • Crotti, A., Lusic, M., Lupo, R., Lievens, P. M. J., Liboi, E., Chiara, G. D., Tinelli, M., Lazzarin, A., Patterson, B. K., Giacca, M., Bovolenta, C., Poli, G. (2007). Naturally occurring C-terminally truncated STAT5 is a negative regulator of HIV-1 expression. Blood 109: 5380-5389 [Abstract] [Full Text]  
  • Moore, F., Buonocore, S., Aksoy, E., Ouled-Haddou, N., Goriely, S., Lazarova, E., Paulart, F., Heirman, C., Vaeremans, E., Thielemans, K., Goldman, M., Flamand, V. (2007). An Alternative Pathway of NF-{kappa}B Activation Results in Maturation and T Cell Priming Activity of Dendritic Cells Overexpressing a Mutated I{kappa}B{alpha}. J. Immunol. 178: 1301-1311 [Abstract] [Full Text]  
  • Varin, A., Decrion, A.-Z., Sabbah, E., Quivy, V., Sire, J., Van Lint, C., Roques, B. P., Aggarwal, B. B., Herbein, G. (2005). Synthetic Vpr Protein Activates Activator Protein-1, c-Jun N-terminal Kinase, and NF-{kappa}B and Stimulates HIV-1 Transcription in Promonocytic Cells and Primary Macrophages. J. Biol. Chem. 280: 42557-42567 [Abstract] [Full Text]  
  • Goffin, V., Demonte, D., Vanhulle, C., de Walque, S., de Launoit, Y., Burny, A., Collette, Y., Van Lint, C. (2005). Transcription factor binding sites in the pol gene intragenic regulatory region of HIV-1 are important for virus infectivity. Nucleic Acids Res 33: 4285-4310 [Abstract] [Full Text]  
  • Aksoy, E., Zouain, C. S., Vanhoutte, F., Fontaine, J., Pavelka, N., Thieblemont, N., Willems, F., Ricciardi-Castagnoli, P., Goldman, M., Capron, M., Ryffel, B., Trottein, F. (2005). Double-stranded RNAs from the Helminth Parasite Schistosoma Activate TLR3 in Dendritic Cells. J. Biol. Chem. 280: 277-283 [Abstract] [Full Text]  
  • Thierry, S., Marechal, V., Rosenzwajg, M., Sabbah, M., Redeuilh, G., Nicolas, J.-C., Gozlan, J. (2004). Cell Cycle Arrest in G2 Induces Human Immunodeficiency Virus Type 1 Transcriptional Activation through Histone Acetylation and Recruitment of CBP, NF-{kappa}B, and c-Jun to the Long Terminal Repeat Promoter. J. Virol. 78: 12198-12206 [Abstract] [Full Text]  
  • Logan, A. C., Haas, D. L., Kafri, T., Kohn, D. B. (2004). Integrated Self-Inactivating Lentiviral Vectors Produce Full-Length Genomic Transcripts Competent for Encapsidation and Integration. J. Virol. 78: 8421-8436 [Abstract] [Full Text]  
  • Henderson, A., Holloway, A., Reeves, R., Tremethick, D. J. (2004). Recruitment of SWI/SNF to the Human Immunodeficiency Virus Type 1 Promoter. Mol. Cell. Biol. 24: 389-397 [Abstract] [Full Text]  
  • Rohr, O., Marban, C., Aunis, D., Schaeffer, E. (2003). Regulation of HIV-1 gene transcription: from lymphocytes to microglial cells. J. Leukoc. Biol. 74: 736-749 [Abstract] [Full Text]  
  • Goriely, S., Demonte, D., Nizet, S., De Wit, D., Willems, F., Goldman, M., Van Lint, C. (2003). Human IL-12(p35) gene activation involves selective remodeling of a single nucleosome within a region of the promoter containing critical Sp1-binding sites. Blood 101: 4894-4902 [Abstract] [Full Text]  
  • Andersen, E. S., Jeeninga, R. E., Damgaard, C. K., Berkhout, B., Kjems, J. (2003). Dimerization and Template Switching in the 5' Untranslated Region between Various Subtypes of Human Immunodeficiency Virus Type 1. J. Virol. 77: 3020-3030 [Abstract] [Full Text]  
  • Varin, A., Manna, S. K., Quivy, V., Decrion, A.-Z., Van Lint, C., Herbein, G., Aggarwal, B. B. (2003). Exogenous Nef Protein Activates NF-kappa B, AP-1, and c-Jun N-Terminal Kinase and Stimulates HIV Transcription in Promonocytic Cells. ROLE IN AIDS PATHOGENESIS. J. Biol. Chem. 278: 2219-2227 [Abstract] [Full Text]  
  • Quivy, V., Adam, E., Collette, Y., Demonte, D., Chariot, A., Vanhulle, C., Berkhout, B., Castellano, R., de Launoit, Y., Burny, A., Piette, J., Bours, V., Van Lint, C. (2002). Synergistic Activation of Human Immunodeficiency Virus Type 1 Promoter Activity by NF-{kappa}B and Inhibitors of Deacetylases: Potential Perspectives for the Development of Therapeutic Strategies. J. Virol. 76: 11091-11103 [Abstract] [Full Text]  
  • Sgarbanti, M., Borsetti, A., Moscufo, N., Bellocchi, M. C., Ridolfi, B., Nappi, F., Marsili, G., Marziali, G., Coccia, E. M., Ensoli, B., Battistini, A. (2002). Modulation of Human Immunodeficiency Virus 1 Replication by Interferon Regulatory Factors. JEM 195: 1359-1370 [Abstract] [Full Text]  
  • Chang, T. L.-Y., Mosoian, A., Pine, R., Klotman, M. E., Moore, J. P. (2002). A Soluble Factor(s) Secreted from CD8+ T Lymphocytes Inhibits Human Immunodeficiency Virus Type 1 Replication through STAT1 Activation. J. Virol. 76: 569-581 [Abstract] [Full Text]  
  • Mahlknecht, U., Deng, C., Lu, M. C., Greenough, T. C., Sullivan, J. L., O'Brien, W. A., Herbein, G. (2000). Resistance to Apoptosis in HIV-Infected CD4+ T Lymphocytes Is Mediated by Macrophages: Role for Nef and Immune Activation in Viral Persistence. J. Immunol. 165: 6437-6446 [Abstract] [Full Text]  
  • Henderson, A., Bunce, M., Siddon, N., Reeves, R., Tremethick, D. J. (2000). High-Mobility-Group Protein I Can Modulate Binding of Transcription Factors to the U5 Region of the Human Immunodeficiency Virus Type 1 Proviral Promoter. J. Virol. 74: 10523-10534 [Abstract] [Full Text]  
  • Beerens, N., Groot, F., Berkhout, B. (2000). Stabilization of the U5-leader stem in the HIV-1 RNA genome affects initiation and elongation of reverse transcription. Nucleic Acids Res 28: 4130-4137 [Abstract] [Full Text]  
  • Jeeninga, R. E., Hoogenkamp, M., Armand-Ugon, M., de Baar, M., Verhoef, K., Berkhout, B. (2000). Functional Differences between the Long Terminal Repeat Transcriptional Promoters of Human Immunodeficiency Virus Type 1 Subtypes A through G. J. Virol. 74: 3740-3751 [Abstract] [Full Text]  
  • Saifuddin, M., Roebuck, K. A., Chang, C.-h., Ting, J. P. Y., Spear, G. T. (2000). Cutting Edge: Activation of HIV-1 Transcription by the MHC Class II Transactivator. J. Immunol. 164: 3941-3945 [Abstract] [Full Text]  
  • Beerens, N., Klaver, B., Berkhout, B. (2000). A Structured RNA Motif Is Involved in Correct Placement of the tRNA3Lys Primer onto the Human Immunodeficiency Virus Genome. J. Virol. 74: 2227-2238 [Abstract] [Full Text]  
  • Trim, J. E., Samra, S. K., Arthur, M. J. P., Wright, M. C., McAulay, M., Beri, R., Mann, D. A. (2000). Upstream Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) Element-1, a Novel and Essential Regulatory DNA Motif in the Human TIMP-1 Gene Promoter, Directly Interacts with a 30-kDa Nuclear Protein. J. Biol. Chem. 275: 6657-6663 [Abstract] [Full Text]  
  • Pereira, L. A., Bentley, K., Peeters, A., Churchill, M. J, Deacon, N. J. (2000). SURVEY AND SUMMARY A compilation of cellular transcription factor interactions with the HIV-1 LTR promoter. Nucleic Acids Res 28: 663-668 [Abstract] [Full Text]  
  • Nakao, K., Nakata, K., Yamashita, M., Tamada, Y., Hamasaki, K., Ishikawa, H., Kato, Y., Eguchi, K., Ishii, N. (1999). p48 (ISGF-3gamma ) Is Involved in Interferon-alpha -induced Suppression of Hepatitis B Virus Enhancer-1 Activity. J. Biol. Chem. 274: 28075-28078 [Abstract] [Full Text]  
  • Kiermer, V., Van Lint, C., Briclet, D., Vanhulle, C., Kettmann, R., Verdin, E., Burny, A., Droogmans, L. (1998). An Interferon Regulatory Factor Binding Site in the U5 Region of the Bovine Leukemia Virus Long Terminal Repeat Stimulates Tax-Independent Gene Expression. J. Virol. 72: 5526-5534 [Abstract] [Full Text]  
  • Emiliani, S., Fischle, W., Ott, M., Van Lint, C., Amella, C. A., Verdin, E. (1998). Mutations in the tat Gene Are Responsible for Human Immunodeficiency Virus Type 1 Postintegration Latency in the U1 Cell Line. J. Virol. 72: 1666-1670 [Abstract] [Full Text]  
  • Sheridan, P. L., Mayall, T. P., Verdin, E., Jones, K. A. (1997). Histone acetyltransferases regulate HIV-1 enhancer activity in vitro. Genes Dev. 11: 3327-3340 [Abstract] [Full Text]  
  • Beerens, N., Groot, F., Berkhout, B. (2001). Initiation of HIV-1 Reverse Transcription Is Regulated by a Primer Activation Signal. J. Biol. Chem. 276: 31247-31256 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»