This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Salanki, K. Articles by Tepfer, M. Search for Related Content PubMed PubMed Citation Articles by Salanki, K. Articles by Tepfer, M.

 Previous Article  |  Next Article 

J. Virol., 05 1997, 3597-3602, Vol 71, No. 5
Copyright © 1997, American Society for Microbiology

Biological properties of pseudorecombinant and recombinant strains created with cucumber mosaic virus and tomato aspermy virus

K Salanki, I Carrere, M Jacquemond, E Balazs and M Tepfer
Agricultural Biotechnology Center, Institute for Plant Sciences, Godollo, Hungary.

Cucumber mosaic virus (CMV) and tomato aspermy virus (TAV) are closely related cucumoviruses. We have made pseudorecombinant viruses in which the RNAs 3 of these two viruses have been exchanged and recombinant viruses containing chimeric RNA 3 molecules, in which the coat proteins and the 3'-end regions of CMV and TAV have been exchanged, giving rise to recombinants designated RT3 and TR3. The replication properties and the cell-to-cell and long-distance movement patterns of these pseudorecombinant and recombinant viruses were examined in different hosts. All the viruses were able to replicate and accumulate RNA 4 in protoplasts. The pseudorecombinants and the R1R2RT3 recombinant infected tobacco systemically, but the R1R2TR3 recombinant was not detectable, even in the inoculated leaves. Comparison of the abilities of the viruses to replicate in protoplasts and intact cucumber plants suggests that cell-to-cell movement factors are also encoded by RNAs 1 and/or 2. Major determinants of symptom severity in Nicotiana glutinosa are localized on the 3' part of RNA 3, and in Nicotiana benthamiana, more severe symptoms were observed with the T1T2R3 strain than with the others tested.

This article has been cited by other articles:

• Thompson, J. R., Buratti, E., de Wispelaere, M., Tepfer, M. (2008). Structural and functional characterization of the 5' region of subgenomic RNA5 of cucumber mosaic virus. J. Gen. Virol. 89: 1729-1738 [Abstract] [Full Text]  
• Turturo, C., Friscina, A., Gaubert, S., Jacquemond, M., Thompson, J. R., Tepfer, M. (2008). Evaluation of potential risks associated with recombination in transgenic plants expressing viral sequences. J. Gen. Virol. 89: 327-335 [Abstract] [Full Text]  
• Thompson, J. R., Doun, S., Perry, K. L. (2006). Compensatory Capsid Protein Mutations in Cucumber Mosaic Virus Confer Systemic Infectivity in Squash (Cucurbita pepo).. J. Virol. 80: 7740-7743 [Abstract] [Full Text]  
• Llamas, S., Moreno, I. M., Garcia-Arenal, F. (2006). Analysis of the viability of coat-protein hybrids between Cucumber mosaic virus and Tomato aspermy virus. J. Gen. Virol. 87: 2085-2088 [Abstract] [Full Text]  
• Salanki, K., Gellert, A., Huppert, E., Naray-Szabo, G., Balazs, E. (2004). Compatibility of the movement protein and the coat protein of cucumoviruses is required for cell-to-cell movement. J. Gen. Virol. 85: 1039-1048 [Abstract] [Full Text]  
• Suzuki, M., Yoshida, M., Yoshinuma, T., Hibi, T. (2003). Interaction of replicase components between Cucumber mosaic virus and Peanut stunt virus. J. Gen. Virol. 84: 1931-1939 [Abstract] [Full Text]  
• Teycheney, P.-Y., Aaziz, R., Dinant, S., Salánki, K., Tourneur, C., Balázs, E., Jacquemond, M., Tepfer, M. (2000). Synthesis of (-)-strand RNA from the 3' untranslated region of plant viral genomes expressed in transgenic plants upon infection with related viruses. J. Gen. Virol. 81: 1121-1126 [Abstract] [Full Text]  
• Belin, C, Schmitt, C, Gaire, F, Walter, B, Demangeat, G, Pinck, L (1999). The nine C-terminal residues of the grapevine fanleaf nepovirus movement protein are critical for systemic virus spread. J. Gen. Virol. 80: 1347-1356 [Abstract]  
• Masuta, C., Ueda, S., Suzuki, M., Uyeda, I. (1998). Evolution of a quadripartite hybrid virus by interspecific exchange and recombination between replicase components of two related tripartite RNA viruses. Proc. Natl. Acad. Sci. USA 95: 10487-10492 [Abstract] [Full Text]