This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chiou, S. K.
Right arrow Articles by White, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chiou, S. K.
Right arrow Articles by White, E.

 Previous Article  |  Next Article 

J. Virol., May 1997, 3515-3525, Vol 71, No. 5
Copyright © 1997, American Society for Microbiology

p300 binding by E1A cosegregates with p53 induction but is dispensable for apoptosis

SK Chiou and E White
Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, New Jersey 08854, USA.

E1A expression during adenovirus infection induces apoptosis. E1A expression causes accumulation of the p53 tumor suppressor protein, and E1A-induced apoptosis is p53 mediated in primary rodent cells, implying that p53 induction may be linked to apoptosis induction by E1A. Adenoviruses containing mutations in the E1A gene were tested for the ability to trigger both p53 accumulation and the appearance of enhanced cytopathy (cyt phenotype) and degradation of DNA (deg phenotype), indicative of apoptosis in infected HeLa cells. The adenoviruses had mutations which disrupted the pRb- and/or p300-binding activities of E1A so that the relationship between p53 induction and apoptosis and binding to these cellular proteins by E1A could be determined. An E1A mutation that specifically disrupted the p300-binding activity failed to induce p53 accumulation, whereas mutations in E1A which affected pRb binding induced p53 accumulation. Thus, p300 binding was required and pRb binding was dispensable for E1A-mediated accumulation of p53 in HeLa cells. All the E1A mutant viruses, regardless of the ability to induce p53 accumulation, induced the cyt and deg phenotypes, suggesting that p53 induction in infected HeLa cells was not essential for apoptosis, nor was binding of E1A to the pRb and/or p300 protein. The possibility that E1A induced a p53-independent apoptosis pathway was tested by analyzing the appearance of the cyt and deg phenotypes in Saos-2 cells, which were null for both alleles of p53, upon adenovirus infection. An adenovirus expressing wild-type 12S E1A induced both the cyt and deg phenotypes in Saos-2 cells, as did all the E1A mutant viruses. Thus, E1A expression during infection of human cells may trigger redundant p53-independent and -dependent apoptotic pathways.


This article has been cited by other articles:

  • Miller, D. L., Rickards, B., Mashiba, M., Huang, W., Flint, S. J. (2009). The Adenoviral E1B 55-Kilodalton Protein Controls Expression of Immune Response Genes but Not p53-Dependent Transcription. J. Virol. 83: 3591-3603 [Abstract] [Full Text]  
  • Shimazu, T., Degenhardt, K., Nur-E-Kamal, A., Zhang, J., Yoshida, T., Zhang, Y., Mathew, R., White, E., Inouye, M. (2007). NBK/BIK antagonizes MCL-1 and BCL-XL and activates BAK-mediated apoptosis in response to protein synthesis inhibition. Genes Dev. 21: 929-941 [Abstract] [Full Text]  
  • Dryer, R. L., Covey, L. R. (2005). A Novel NF-{kappa}B-Regulated Site within the Human I{gamma}1 Promoter Requires p300 for Optimal Transcriptional Activity. J. Immunol. 175: 4499-4507 [Abstract] [Full Text]  
  • Samuelson, A. V., Narita, M., Chan, H.-M., Jin, J., de Stanchina, E., McCurrach, M. E., Narita, M., Fuchs, M., Livingston, D. M., Lowe, S. W. (2005). p400 Is Required for E1A to Promote Apoptosis. J. Biol. Chem. 280: 21915-21923 [Abstract] [Full Text]  
  • Rasti, M., Grand, R. J. A., Mymryk, J. S., Gallimore, P. H., Turnell, A. S. (2005). Recruitment of CBP/p300, TATA-Binding Protein, and S8 to Distinct Regions at the N Terminus of Adenovirus E1A. J. Virol. 79: 5594-5605 [Abstract] [Full Text]  
  • Cuconati, A., Mukherjee, C., Perez, D., White, E. (2003). DNA damage response and MCL-1 destruction initiate apoptosis in adenovirus-infected cells. Genes Dev. 17: 2922-2932 [Abstract] [Full Text]  
  • Grossman, S. R., Deato, M. E., Brignone, C., Chan, H. M., Kung, A. L., Tagami, H., Nakatani, Y., Livingston, D. M. (2003). Polyubiquitination of p53 by a Ubiquitin Ligase Activity of p300. Science 300: 342-344 [Abstract] [Full Text]  
  • Perez, D., White, E. (2003). E1A Sensitizes Cells to Tumor Necrosis Factor Alpha by Downregulating c-FLIPS. J. Virol. 77: 2651-2662 [Abstract] [Full Text]  
  • Edwards, S. J., Dix, B. R., Myers, C. J., Dobson-Le, D., Huschtscha, L., Hibma, M., Royds, J., Braithwaite, A. W. (2002). Evidence that Replication of the Antitumor Adenovirus ONYX-015 Is Not Controlled by the p53 and p14ARF Tumor Suppressor Genes. J. Virol. 76: 12483-12490 [Abstract] [Full Text]  
  • Harada, J. N., Shevchenko, A., Shevchenko, A., Pallas, D. C., Berk, A. J. (2002). Analysis of the Adenovirus E1B-55K-Anchored Proteome Reveals Its Link to Ubiquitination Machinery. J. Virol. 76: 9194-9206 [Abstract] [Full Text]  
  • Cuconati, A., Degenhardt, K., Sundararajan, R., Anschel, A., White, E. (2002). Bak and Bax Function To Limit Adenovirus Replication through Apoptosis Induction. J. Virol. 76: 4547-4558 [Abstract] [Full Text]  
  • Holm, P. S., Bergmann, S., Jurchott, K., Lage, H., Brand, K., Ladhoff, A., Mantwill, K., Curiel, D. T., Dobbelstein, M., Dietel, M., Gansbacher, B., Royer, H.-D. (2002). YB-1 Relocates to the Nucleus in Adenovirus-infected Cells and Facilitates Viral Replication by Inducing E2 Gene Expression through the E2 Late Promoter. J. Biol. Chem. 277: 10427-10434 [Abstract] [Full Text]  
  • Querido, E., Blanchette, P., Yan, Q., Kamura, T., Morrison, M., Boivin, D., Kaelin, W. G., Conaway, R. C., Conaway, J. W., Branton, P. E. (2001). Degradation of p53 by adenovirus E4orf6 and E1B55K proteins occurs via a novel mechanism involving a Cullin-containing complex. Genes Dev. 15: 3104-3117 [Abstract] [Full Text]  
  • Chattopadhyay, D., Ghosh, M. K., Mal, A., Harter, M. L. (2001). Inactivation of p21 by E1A Leads to the Induction of Apoptosis in DNA-Damaged Cells. J. Virol. 75: 9844-9856 [Abstract] [Full Text]  
  • Querido, E., Morisson, M. R., Chu-Pham-Dang, H., Thirlwell, S. W.-L., Boivin, D., Branton, P. E. (2001). Identification of Three Functions of the Adenovirus E4orf6 Protein That Mediate p53 Degradation by the E4orf6-E1B55K Complex. J. Virol. 75: 699-709 [Abstract] [Full Text]  
  • Marcellus, R. C., Chan, H., Paquette, D., Thirlwell, S., Boivin, D., Branton, P. E. (2000). Induction of p53-Independent Apoptosis by the Adenovirus E4orf4 Protein Requires Binding to the Balpha Subunit of Protein Phosphatase 2A. J. Virol. 74: 7869-7877 [Abstract] [Full Text]  
  • Shao, R., Xia, W., Hung, M.-C. (2000). Inhibition of Angiogenesis and Induction of Apoptosis Are Involved in E1A-mediated Bystander Effect and Tumor Suppression. Cancer Res. 60: 3123-3126 [Abstract] [Full Text]  
  • Medina, D. J., Sheay, W., Goodell, L., Kidd, P., White, E., Rabson, A. B., Strair, R. K. (1999). Adenovirus-Mediated Cytotoxicity of Chronic Lymphocytic Leukemia Cells. Blood 94: 3499-3508 [Abstract] [Full Text]  
  • Harada, J. N., Berk, A. J. (1999). p53-Independent and -Dependent Requirements for E1B-55K in Adenovirus Type 5 Replication. J. Virol. 73: 5333-5344 [Abstract] [Full Text]  
  • Fernandes, E. R., Rooney, R. J. (1999). Suppression of E1A-Mediated Transformation by the p50E4F Transcription Factor. Mol. Cell. Biol. 19: 4739-4749 [Abstract] [Full Text]  
  • Turnell, A. S., Grand, R. J. A., Gallimore, P. H. (1999). The Replicative Capacities of Large E1B-Null Group A and Group C Adenoviruses Are Independent of Host Cell p53 Status. J. Virol. 73: 2074-2083 [Abstract] [Full Text]  
  • Boivin, D., Morrison, M. R., Marcellus, R. C., Querido, E., Branton, P. E. (1999). Analysis of Synthesis, Stability, Phosphorylation, and Interacting Polypeptides of the 34-Kilodalton Product of Open Reading Frame 6 of the Early Region 4 Protein of Human Adenovirus Type 5. J. Virol. 73: 1245-1253 [Abstract] [Full Text]  
  • Gottifredi, V, Peschiaroli, A, Fimia, G., Maione, R (1999). p53-independent apoptosis induced by muscle differentiation stimuli in polyomavirus large T-expressing myoblasts. J. Cell Sci. 112: 2397-2407 [Abstract]  
  • Mulloy, J. C., Kislyakova, T., Cereseto, A., Casareto, L., LoMonico, A., Fullen, J., Lorenzi, M. V., Cara, A., Nicot, C., Giam, C.-Z., Franchini, G. (1998). Human T-Cell Lymphotropic/Leukemia Virus Type 1 Tax Abrogates p53-Induced Cell Cycle Arrest and Apoptosis through Its CREB/ATF Functional Domain. J. Virol. 72: 8852-8860 [Abstract] [Full Text]  
  • Han, J., Wallen, H. D., Nuñez, G., White, E. (1998). E1B 19,000-Molecular-Weight Protein Interacts with and Inhibits CED-4-Dependent, FLICE-Mediated Apoptosis. Mol. Cell. Biol. 18: 6052-6062 [Abstract] [Full Text]  
  • Nakajima, T., Yageta, M., Shiotsu, K., Morita, K., Suzuki, M., Tomooka, Y., Oda, K. (1998). Suppression of adenovirus E1A-induced apoptosis by mutated p53 is overcome by coexpression with Id proteins. Proc. Natl. Acad. Sci. USA 95: 10590-10595 [Abstract] [Full Text]  
  • Nakajima, T., Morita, K., Tsunoda, H., Imajoh-Ohmi, S., Tanaka, H., Yasuda, H., Oda, K. (1998). Stabilization of p53 by Adenovirus E1A Occurs through Its Amino-terminal Region by Modification of the Ubiquitin-Proteasome Pathway. J. Biol. Chem. 273: 20036-20045 [Abstract] [Full Text]  
  • Thomas, A., White, E. (1998). Suppression of the p300-dependent mdm2 negative-feedback loop induces the p53 apoptotic function. Genes Dev. 12: 1975-1985 [Abstract] [Full Text]  
  • Li, Z., Yu, A., Weiner, A. M. (1998). Adenovirus Type 12-Induced Fragility of the Human RNU2 Locus Requires p53 Function. J. Virol. 72: 4183-4191 [Abstract] [Full Text]  
  • Samuelson, A. V., Lowe, S. W. (1997). Selective induction of p53 and chemosensitivity in RB-deficient cells by E1A mutants unable to bind the RB-related proteins. Proc. Natl. Acad. Sci. USA 94: 12094-12099 [Abstract] [Full Text]  
  • Zhu, Q., Yao, J., Wani, G., Wani, M. A., Wani, A. A. (2001). Mdm2 Mutant Defective in Binding p300 Promotes Ubiquitination but Not Degradation of p53. EVIDENCE FOR THE ROLE OF p300 IN INTEGRATING UBIQUITINATION AND PROTEOLYSIS. J. Biol. Chem. 276: 29695-29701 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»